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Citations for New England Biolabs :
1 - 50 of 1458 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... all sequences between transposable elements of a p2T plasmid were removed by restriction digestion using AleI and EcoRI (NEB). The TetO-eGFP-HygroR cassette was created by amplifying each subunit individually from already excising constructs ...
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bioRxiv - Microbiology 2022Quote: ... The library of circular DNA fragments was then used as a PCR template with forward and reverse primers specific to the transposable element and amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs). PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research ...
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bioRxiv - Biochemistry 2023Quote: ... to pcDNA4/TO and PiggyBac-CMV-MCS-IRES-mCherry and PiggyBac-CMV-MCS-IRES-mNeongreen by PCR using Q5 High Fidelity 2X mastermix (NEB). PiggyBac-CMV-MCS-IRES-NLS-TagBFP was used as empty vector control ...
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bioRxiv - Developmental Biology 2022Quote: ... The bicistronic reporters used to detect regulatory elements within 3′ UTRs were constructed by Gibson Assembly (NEB) and standard methods ...
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bioRxiv - Genomics 2024Quote: ... coli (derived from NEB C3040 and prepared according to Nováková et al.14 ...
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bioRxiv - Microbiology 2021Quote: The chimeric CrPV-aNCV infectious clone was derived from the full-length CrPV infectious clone (pCrPV-3; Accession KP974707) (35) using Gibson assembly (New England BioLabs), per the manufacturer's instructions ...
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bioRxiv - Neuroscience 2020Quote: Illumina compatible libraries were made from Visium derived cDNA using a modified protocol derived from NEB Ultra II DNA FS kit (NEB #E6177) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201] ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-miRNAs were purified on denaturant polyacrylamide gel and long pri-miR-K10/12 derived transcripts (up to ∼3 kb) were salt purified using Monarch® PCR and DNA cleanup kit (New England BioLabs). After acidic phenol extraction and ethanol precipitation ...
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bioRxiv - Cell Biology 2023Quote: ... mCh-Rab7A was cloned into a piggyBac plasmid using the NEB HIFI DNA Assembly Kit (NEB) and primers listed in Supp ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 1 h at 37°C into the PiggyBac recipient vector previously digested with BlpI (NEB #R0585S) and BstXI (NEB #R0113S ...
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bioRxiv - Molecular Biology 2023Quote: ... Stable cell lines were generated by transfection of piggyBac transposon (PB) and transposase (PBO, Transposagen/Hera BioLabs) plasmids with GeneJuice (Novogene ...
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bioRxiv - Bioengineering 2024Quote: pBmhsp90:hyPB was generated by inserting the Bombyx mori hsp90 2.9 kb fragment from pBmhsp90:GFP/3xP3:DsRed and the hyper-active Piggybac transposase from SPB-DNA (Hera Biolabs) upstream of wild type PiggyBac transposase 3’ UTR from pHA3PIG in the digested backbone of pHA3PIG.
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bioRxiv - Biochemistry 2022Quote: ... The following cloning strains were used: NEB Stable (lentiviral and piggybac vectors) and NEB 5-alpha (all other plasmids) (New England Biolabs). For cloning of base editor constructs ...
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bioRxiv - Biochemistry 2023Quote: ... The pCR8[mZC3H18] plasmid was used as a template to generate subsequent ZC3H18 cDNA constructs that were cloned into a piggyBAC (pB) vector containing an N-terminal MYC tag and BSD selection marker using NEBbuilder HiFI DNA assembly (NEB). OsTIR1-HA ...
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bioRxiv - Molecular Biology 2024Quote: ... These were then used as templates to introduce into a piggyBAC (pB) vector containing an N- terminal MYC and miniTurbo (MYC-mTurbo) tag using NEBbuilder HiFi DNA assembly (NEB). Wildtype mES cells were transfected with pB[MYC-] or pB[MYC-mTurbo-PHAX] vectors along with a piggyBAC transposase expressing vector (pBase ...
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bioRxiv - Systems Biology 2022Quote: ... The SNAP tag insert was derived from pSNAPf (NEB, N9183S) and the FRB-Fis1 insert was derived from pC4-RhE-FRB-Fis1 ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... These target genes were constructed into a modified PiggyBac (PB) transposon vector EIRBsMie using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621L) described previously (44).
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bioRxiv - Molecular Biology 2021Quote: ... The SCR and its truncated version (tSCR or Ei) were cloned between the piggyBac transposon-specific inverted terminal repeat sequences (ITR) by linearizing the vector with BamHI (NEB, R3136) and NheI (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... A selection cassette carrying the Puromycin resistance gene driven by the PGK promoter and flanked by FRT sites was cloned in front of the Sox2 promoter by linearizing the piggyBac vector with the AsiSI (NEB, R0630) restriction enzyme ...
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bioRxiv - Genomics 2024Quote: ... Purified product was Gibson cloned into a previously described PiggyBac transposon vector (37) using standard protocols in a 10 μl reaction (NEB E2621S). 2.5 μl of the Gibson reaction was electroporated into 25 μl of 10-beta electrocompetent E ...
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bioRxiv - Cell Biology 2024Quote: ... GCTGT CAGTT CAGGT TTCTT CATGG TGGGT GGGCC AGGAT TCTCC TCCAC GTCAC CGCAT GTTAG GAGAC TTCCT CTGCC CTCCC CGGAA CCCCC ACCGC CGGAA ATCTC GAGCG TGG The piggyBac transposon vector was digested with NheI-HF (NEB, R3131S) and a two-fragment Gibson assembly was carried out ...
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bioRxiv - Molecular Biology 2020Quote: ... ampicillin resistance gene (Amp) was derived from pUC19 (New England Biolabs) whereas gene expression cassette were amplified from and pET30a (Novagen ...
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bioRxiv - Cell Biology 2024Quote: ... coli-derived recombinant human histone H1 (NEB, Ipswich, MA, USA #M2501), H2A (NEB ...
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bioRxiv - Biophysics 2024Quote: M13mp18-derived p7249 scaffold was obtained from NEB (catalog no. N4040S). Unmodified and biotinylated oligonucleotides for the rectangle origamis were obtained from MWG Eurofins ...
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bioRxiv - Microbiology 2021Quote: ... amplified mini-CRISPR elements were digested by KpnI and BamHI (NEB, USA) and were ligated into pAY5211 employing the Quick LigationTM Kit (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... mTagBFP2 and Cas9-Avidin provided by Max Wilson into an expression vector bearing a CMV promoter and flanking PiggyBac transposase-compatible inverted terminal repeats using Gibson Assembly (New England BioLabs Inc., E2611L) according to supplier instructions ...
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bioRxiv - Developmental Biology 2023Quote: Regulatory elements were amplified from mouse genomic DNA with Q5 polymerase (NEB, M0491) using primers listed in Table S8 and cloned into pGL4.24[luc2P/minP] (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... a construct containing the following DNA elements was assembled by HiFi cloning (NEB): (1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and cell pellets from our patient-derived cell lines was extracted (NEB, T3010S). Genomic libraries were prepared (Illumina ...
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bioRxiv - Genomics 2020Quote: Sequencing libraries were prepared using DNA derived from Enterobacteria phage lambda (NEB N3011S), fragmented using a g-TUBE™(Covaris® 520079 ...
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bioRxiv - Molecular Biology 2022Quote: ... derived from the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). RNA was then capped with 3′-Desthiobiotin-GTP and polyA-tailed using vaccinia capping enzyme and E ...
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bioRxiv - Biophysics 2021Quote: ... according to the protocol for cloning DNA containing repeat elements (C3040, New England BioLabs Inc.). In both plasmids ...
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bioRxiv - Cancer Biology 2021Quote: ... or a polyclonal rabbit antibody against cleaved casp-3 (1:100; New England Biolabs, Frankfurt, Germany), followed by a biotinylated goat anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and 7f variants were derived by site-directed mutagenesis (NEB Q5 Site-Directed Mutagenesis Kit) of the GCaMP6s sequence in pDSP23 to reproduce the mutations described for GCaMP6f (pDSP31 ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
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bioRxiv - Genomics 2024Quote: ... The nodule-derived RNA was treated with NEBNext rRNA Depletion Kit (Bacteria) (NEW ENGLAND BioLabs) and RiboMinus Plant Kit for RNA-Seq (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... was expressed from (pET28a-derived) plasmid pED72 in Escherichia coli ER2566 cells (New England Biolabs, fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL Klenow fragment (3’→5’ exo-) (NEB), and 9 μL of DEPC H2O to each reaction and incubating at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies: Rabbit anti-Yap1 (Cat# 14074S; 1:300) and Rabbit anti-Smad2/3 (NEB #8685S; 1:300).
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bioRxiv - Molecular Biology 2022Quote: ... The substrate was obtained by PCR on CML cells derived gDNA using Q5 DNA polymerase (NEB) and purified on a 10g/L agarose gel using the Wizard SV Gel and PCR Clean-Up system (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... were derived from pESN17 in an equivalent manner using the NEBuilder HiFi DNA assembly kit (NEB). Plasmid pESN69 derived from the assembly of PCR products ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was derived from total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S). Numerous primers were designed against cryptic exon targets and screened to identify primer pairs that minimized background bands.
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bioRxiv - Biophysics 2021Quote: ... Two of them correspond to two PCR-fabricated DNAs (C1 and C2) derived from lambda DNA (NEB) as template and including suitable restriction sites in the designed primers (Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... a custom 3’ adapter (5’-rAppCTGTAGGCACCATCAAT–NH2-3’, NEB, S1315S) was ligated to all RNAs ...
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bioRxiv - Biochemistry 2021Quote: ... and C250 (derived from pX461, GFP replaced with dsRED (Lindner et al. 2017)) were digested with BbsI (NEB) and ligated with the following annealed oligomers:
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bioRxiv - Developmental Biology 2022Quote: ... Patient-derived variants were created by site-directed mutagenesis using Q5 High Fidelity Master Mix (NEB Catalog #M0492L), T4 polynucleotide kinase (NEB Catalog# M0201L ...
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bioRxiv - Immunology 2023Quote: ... The pEGIP-derived packaging vector pEGIP-hACE2 was generated by homologous recombination using the NEBuilder kit (NEB, E5520). The vector backbone was amplified using oligonucleotides with appropriate hACE2 sequence overhangs ...
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bioRxiv - Microbiology 2024Quote: ... a plasmid derived from pUC19 plasmid previously prepared in our laboratory was digested with KpnI and PshAI (NEB) followed by dephosphorylation ...
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bioRxiv - Molecular Biology 2024Quote: ... Five micrograms of genomic DNA derived from G418R colonies were digested to completion with SspI or ApoI (NEB) in a total reaction volume of 50 μL ...