Labshake search
Citations for New England Biolabs :
1 - 50 of 698 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... the 5’ end of the 4 kb transcript was biotin-labeled using Vaccinia Capping System (NEB) and 3-biotin-GTP (NEB ...
-
bioRxiv - Biophysics 2020Quote: ... and 3-biotin-GTP (NEB) and purified using MEGAclear ...
-
bioRxiv - Biophysics 2021Quote: CoA-biotin (New England Biolabs) was coupled to the ybbR-tag of the VWF-dimer constructs in a bulk reaction in the presence of 5 μM sfp phosphopantetheinyl transferase53 and 10 mM MgCl2 at 37 °C for 60 min ...
-
bioRxiv - Biophysics 2020Quote: CoA-biotin (New England Biolabs) was coupled to the ybbR-tag at the C-terminus of the fusion protein constructs in a 90 min bulk reaction in the presence of 4 µM sfp phosphopantetheinyl transferase63 and 100 mM MgCl2 at room temperature (≈ 22°C) ...
-
bioRxiv - Biophysics 2023Quote: Biotin-SNAP-mDia1 prepared by incubating purified SNAP-mDia1 with Benzylguanine-Biotin (New England Biolabs, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... the biotin-Chd1p was prepared by mixing the SNAP-Chd1p and SNAP-biotin® (New England BioLabs) in a 1:2 molar ratio and incubated at room temperature for 30min ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 1 μL of 10 mM biotin (NEB) was then added to each sample on the plate and incubated for an additional five minutes to compete away purely biotin-dependent interactions ...
-
bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD-SNAP was biotinylated or fluorescently labelled by overnight incubation at 4°C with SNAP-Biotin® or SNAP-Surface® Alex Fluor® 647 (New England BioLabs) with at least a 3:1 molar excess of these labels to PfK5ΔL6-MD-SNAP ...
-
bioRxiv - Genomics 2021Quote: DNA ends were marked with biotin-14-dATP by adding 60μL of biotin fill-in master mix (1X NEB 3.1 ...
-
bioRxiv - Systems Biology 2021Quote: ... the purified DNAs were annealed using random nonamer primers with a 5′-biotin tag (5′-Biotin-CTACACGACGCTCTTCCGATCTNNNNNNNNN-3′) in the presence of Klenow fragments (3′-5′ exo-, New England Biolabs). Then ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein blots were incubated with primary antibodies overnight at 4°C using the above-mentioned antibodies and the Gaussia Rabbit Polyclonal Antibody (E8023S, New England BioLabs), the Influenza A NS1 Mouse Monoclonal Antibody (sc-130568 ...
-
bioRxiv - Cell Biology 2024Quote: ... SNAP-Cell TMR-Star or SNAP-Biotin (NEB) as described previously65,66.
-
bioRxiv - Synthetic Biology 2020Quote: ... BG-fluorescein or BG-biotin conjugates (known as “SNAP-Surface® 649”, “SNAP-Cell® Fluorescein” and “SNAP-Biotin” respectively, NEB, at a 1:500 dilution ...
-
bioRxiv - Genetics 2023Quote: ... using a biotin-labeled 30 μM dNTP mix (dATP, dGTP, dTTP and Biotin-14-dCTP Thermo Fischer, 19518018) and Klenow enzyme (NEB, M0210L) at 37°C for 80 min ...
-
bioRxiv - Genomics 2020Quote: ... Biotin labeled dATP (Thermo,19524016) and Klenow (NEB, M0210) were used to fill restriction fragment overhangs ...
-
bioRxiv - Molecular Biology 2021Quote: ... were then combined with 10 μM CLIP-Biotin (New England Biolabs) and rotated (end-over-end ...
-
bioRxiv - Genomics 2023Quote: ... dTTP and biotin-dATP using Klenow DNA polymerase I (NEB M0212L), followed by in situ proximity ligation at room temperature overnight ...
-
bioRxiv - Biophysics 2022Quote: ... rk430-mScarlet-SNAP (7 μM monomer) was incubated with benzylguanine-biotin (NEB) in a 4 to 1 molar ratio at room temperature for 15 minutes ...
-
bioRxiv - Synthetic Biology 2020Quote: ... SNAP reagents (BG-649, BG-fluorescein and BG-biotin) were obtained from NEB. Electrocompetent cells were used in all transformations.
-
bioRxiv - Cell Biology 2021Quote: ... and biotin-pupE labelled proteins were enriched by streptavidin magnetic beads (NEB, S1420S) and identified by mass spectrometry analysis (Liu et al. ...
-
bioRxiv - Biophysics 2023Quote: ... The biotin-modified DNA was digested using a KasI restriction enzyme (NEB #R0544S) that produced two fragments of 24 kb and 24.5 kb ...
-
bioRxiv - Genomics 2024Quote: ... The biotin-filled DNA fragments were ligated by T4 DNA ligase (NEB,M0202L) for 4 h at 16 °C ...
-
bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed overnight under rotation at 4 using 1/100 T7RNA antibody (Biolabs CB MAB-0296MC) and antiflag (Sigma F1804 and F3165) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Digested fragments were biotin-labeled and subsequently ligated by T4 DNA ligase buffer (NEB) for 2 hours at 23°C with 300 rpm gentle rotation ...
-
bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
-
bioRxiv - Microbiology 2022Quote: ... The remaining volume was submitted for biotin-enrichment on magnetic Streptavidin beads (NEB S1420S), by vortexing for 90 min at RT ...
-
bioRxiv - Cancer Biology 2022Quote: ... and biotin-labelled proteins were isolated by immunoprecipitation using streptavidin magnetic beads (NEB, S1420S) in an immunoprecipitation buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Genetics 2023Quote: ... biotin from un-ligated DNA ends was removed using T4 DNA polymerase (NEB #M0203) and the DNA was sonicated (Covaris E220 ...
-
bioRxiv - Neuroscience 2024Quote: ... biotin was removed from non-ligated DNA ends in a T4 polymerase (NEB, M0203L) reaction and again subjected to phenol-chloroform purification ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 million permeabilized cells (without any antibodies bound) were incubated with 4 units of Dam enzyme (NEB, M0222L) during the activation step ...
-
bioRxiv - Cancer Biology 2020Quote: ... extra Biotin-14-dATP was first removed using 15 units of T4 DNA polymerase (NEB) and incubating at 20°C for 4 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.167 mg/mL MTSEA-biotin) for 30 min and purified with Streptavidin Magnetic Beads (NEB). The biotinylated RNA was eluted with 100 mM DTT and purified with RNA Clean beads (Vazymes) ...
-
bioRxiv - Molecular Biology 2024Quote: The biotin-enriched eluate was next subjected to 3’end dephosphorylation using PNK (M0201L, NEB) and FastAP (EF0654 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.5% Triton X-100) was split into two tubes and incubated overnight with 4 µl of S9.6 antibody (New England Biolabs) while rotating ...
-
bioRxiv - Genomics 2020Quote: ... biotin-dNTPs at the DNA termini were removed with 500 units of Exonuclease III (NEB #M0206) in 1X NEBuffer 1 (NEB #B7001 ...
-
bioRxiv - Biophysics 2020Quote: ... A 478 bp fragment of biotin labelled DNA was produced by PCR with Taq Polymerase (NEB) of λ-DNA using the following primers ...
-
bioRxiv - Microbiology 2020Quote: ... Biotin-labeled RNP lysates were supplemented with streptavidin beads (1 mL per replicate, New England Biolabs) and captured by rotating at room temperature overnight ...
-
bioRxiv - Genomics 2021Quote: ... biotin was removed from the unligated ends by adding 5 µl of T4 DNA polymerase (NEB) to 5 ug of DNA ...
-
bioRxiv - Biochemistry 2024Quote: ... Purified SNAP-CP was incubated with 5x excess of SNAP-surface-Biotin (New England Biolabs, USA) overnight at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... buffer 4 (NEB, identical composition to rCutsmart buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Biotin-dNTPs at the unligated DNA termini were removed by ∼500 units of Exonuclease III (NEB #M0206) in 1X NEBuffer 1 (NEB #B7001 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1.5 ul 10 mM dGTP, 1.5 ul 10 mM dTTP, 37.5 ul 0.4 mM biotin-14-dATP, 40 ul NEB buffer2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Digested DNA fragments were filled in with biotin-labeled dATP by incubating with Klenow enzyme (NEB, M0212), biotin-14-dATP ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 µl of 1 mM BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 µl of axonemes ...
-
bioRxiv - Molecular Biology 2023Quote: ... SNAP-tag coupling activity (methodology described below) was first tested with a small ligand BG-biotin (NEB) or DNA (a 550 bp benzylguanylated PCR product ...
-
bioRxiv - Genomics 2024Quote: ... Digested DNA fragments were filled in with biotin-labeled dATP by incubating with Klenow enzyme (NEB, M0212), biotin-14-dATP (Thermo Fisher ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 4: 4°C hold) using NEB Next High-Fidelity master mix (NEB) in 25 µl reaction volume using RAD-Marker for/ RAD-Marker rev primers (25 nM each ...
-
bioRxiv - Cell Biology 2020Quote: ... Oct-4 (NEB, D7O5Z), Sox2 (NEB ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 ml ScaI (BioLabs), followed by 3 h of incubation with a fresh portion ...
-
bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...