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Citations for New England Biolabs :
1 - 50 of 194 citations for Persistent Pesticide Calibration Solution Cs1 Cs8 Unlabeled 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... or additional unlabeled ATP (NEB) were added to each reaction as indicated ...
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bioRxiv - Developmental Biology 2023Quote: ... and a capture sequence at the scaffold of sgRNA for 10x feature barcode retrieval (cs1 incorporated at the 3’ end; (Replogle et al., 2020)) with use of NEBuilder HiFi DNA Assembly (NEB, E2621). sgRNAs were designed using CRISPick for CRISPRko (Doench et al. ...
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bioRxiv - Biochemistry 2019Quote: ... unlabeled AdoMet was left out and 6 μg of MBP2* protein (NEB) was added to each reaction to increase molecular crowding.
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bioRxiv - Molecular Biology 2023Quote: Unlabeled purified RNA targets were dephosphorylated using 1 U antarctic phosphatase (NEB) incubated with 1X of provided buffer during 20 min at 37°C and then 5 min at 65°C to inactivate the enzyme ...
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bioRxiv - Biophysics 2023Quote: ... the digoxigenin-labeled and unlabeled amplicons were ligated with T7 ligase (New England BioLabs) in a specific ratio of 1:2 ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were cooled to 4°C for 30 seconds and quenched with 1.2 mM unlabeled SAM (NEB) and blotted onto Hybond-XL membrane ...
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bioRxiv - Genomics 2022Quote: ... the 1:99 target:background sample was digested with FspEI (New England Biolabs) according to the manufacturers protocol (incubation at 37°C for 90 minute ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng of unlabeled crRNA was mixed with equal volume of 2 × RNA loading dye (New England Biolabs) and fractionated in the 12% denaturing polyacrylamide gel ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was PCR-amplified for 25 cycles with 5’-Cy3-labelled reverse primers (IDT) and unlabeled forward primers using either Taq polymerase or Phusion high-fidelity polymerase (NEB). PCR products were separated on 40cm tall 6% polyacrylamide denaturing gels and then visualized using a Molecular Dynamics Typhoon Scanner ...
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bioRxiv - Developmental Biology 2021Quote: DNA probes were generated by annealing 5’ IRDye®700 labeled forward oligonucleotides with unlabeled reverse oligonucleotides (Integrated DNA Technologies) to a final concentration of 5 μM in PNK buffer (New England Biolabs). One hundred femtomoles of labeled IRDye®700 probes were used in a 20-μl binding reaction containing 10 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... and the radiolabeled 3’ fragment was ligated to an unlabeled 5’ fragment by splinted ligation with T4 DNA ligase (NEB) at 30°C for 2 h ...
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bioRxiv - Bioengineering 2019Quote: ... Unlabeled tracrRNAs were synthesized by IDT or through in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB) using DNA templates containing a T7 promoter binding sequence and full length tracrRNAs ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid length DNA binding experiments were performed with unlabeled circular or linearized M13mp18 single (New England Biolabs, M13mp18 single-stranded DNA) or double-stranded DNA (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: ... All experiments for the calibration curve with Influenza and SARS-CoV-2 were performed with the Colorimetric LAMP mix from NEB. Real-time colorimetric LAMP (qcLAMP ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration in the growth medium was quantified by measuring fluorescence at 610 nm following excitation at 575 nm in comparison of signals with a calibration curve prepared from mCherry which was cloned into pQE30 (NEB), expressed in E ...
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bioRxiv - Developmental Biology 2022Quote: ... Histone yields from each purification were determined by using a calibration curve of recombinant H3 and H4 (NEB, cat. #M2503S, #M2504S, respectively). Absolute amounts of histone were determined by densitometry on coomassie-stained bands with Fiji61 for Pristionchus data (conducted at the MPI in Tübingen ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PURExpress® kit containing Solution A and Solution B (NEB) was used to prepare the transcription-translation reaction ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Synthetic Biology 2024Quote: ... 3.2 μL solution A (NEB), 1 μL factor mix (NEB) ...
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bioRxiv - Microbiology 2019Quote: ... The reaction solution was prepared in the following order: 10 μL solution A (NEB #E6800), 7.5 μL solution B (NEB #E6800) ...
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bioRxiv - Microbiology 2019Quote: ... 7.5 μL solution B (NEB #E6800), 2 μL E ...
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bioRxiv - Plant Biology 2020Quote: ... and 30X Enzyme Activator Solution (NEB). Digestion was verified on a TapeStation 2200 with a D1000 ScreenTape ...
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bioRxiv - Molecular Biology 2019Quote: ... coli DNA ligase buffer solution (NEB) to a final volume of 200 μl by heating the solution to 95°C for 2 minutes and then cooling the reaction on ice for 2 minutes ...
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bioRxiv - Immunology 2020Quote: ... and Ribonucleotide Solution Set (NEB, USA). During development phase ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution DNase (NEB) digestions were performed ...
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bioRxiv - Immunology 2024Quote: ... Deoxynucleotide (dNTP) Solution Mix (N0447L, NEB); Dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2020Quote: ... deoxynucleotide solution mix (dNTP, 2.5 mM, NEB) and Phusion HF Buffer (1x ...
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bioRxiv - Biochemistry 2020Quote: ... deoxynucleotide solution mix (dNTP, 2.5 mM, NEB) and Phusion HF Buffer (1x ...
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bioRxiv - Cell Biology 2022Quote: ... 1.25µl of EnGen Cas9 NLS solution (NEB), 1.75 µl H2O ...
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bioRxiv - Bioengineering 2023Quote: ... 1.4 mM dNTP solution (New England Biolabs), 1.6 μM FIP/BIP ...
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bioRxiv - Synthetic Biology 2022Quote: ... 8 μL of solution A and 6 μL of solution B were mixed with 16 U RNase Inhibitor (NEB), gBlock templates (2 nM each ...
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bioRxiv - Synthetic Biology 2023Quote: ... The lipid film was rehydrated by vortexing with 21 μL rehydration solution (14 μL PURExpress Solution A, 0.875 μL RNase Inhibitor (NEB), 17 μM IGEPAL (90mM) ...
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bioRxiv - Genomics 2019Quote: ... 0.12 µL of 30x enzyme activator solution (NEB), 0.05 µL of 10x NEB Buffer 4 and 0.31 µL of nuclease-free water ...
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bioRxiv - Systems Biology 2021Quote: ... 0.4μL 10 mM dNTP Solution (New England Biolabs), 4μL 5x Phusion HF Buffer (Thermo Scientific) ...
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bioRxiv - Genomics 2019Quote: ... and 1 µl Deoxynucleotide Solution Mix (N0447, NEB). Samples were mixed by tapping ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μM dNTP Solution Mix (New England Biolabs), and a concentration of biotin-11-dNTPs (PerkinElmer ...
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bioRxiv - Molecular Biology 2023Quote: Proteinase solution: 4 μL proteinase K (NEB, P8107S) was added into 1 mL 0.1 M Tris-HCl 0.05 M EDTA (pH 8.0 ...
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bioRxiv - Developmental Biology 2022Quote: ... 20μl of extraction solution (25μl of PK (NEB) /ml of NP40 lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... with the following: 3.2 μL solution A (NEB), 1 μL factor mix (NEB) ...
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bioRxiv - Genomics 2023Quote: ... 200 μM dNTP Solution Mix (New England Biolabs), Q5® High GC Enhancer ...
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bioRxiv - Genomics 2023Quote: ... 1.5 μL 1:1,249 diluted Fe2+ solution (NEB), and 8.4 μL water ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5μl Deoxynucleotide (dNTP) Solution Mix (New England Biolabs), 0.5μl P5 primer pool ...
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bioRxiv - Genomics 2023Quote: ... 3 μL 1:1,249 diluted Fe2+ solution (NEB)) at 37°C for 1 h ...
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bioRxiv - Systems Biology 2020Quote: ... 0.4 μL 10 mM dNTP Solution (New England Biolabs), 4 μL 5x Phusion HF Buffer (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... and deoxynucleotide solution (both from New England Biolabs, NEB). The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB) ...
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bioRxiv - Microbiology 2022Quote: ... and deoxynucleotide solution (both from New England Biolabs, NEB). The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 uL dNTP Solution Mix (10 mM stock, NEB), 2 μl Taq DNA ligase (80 U ...
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bioRxiv - Immunology 2023Quote: ... and 25ul 10X DNaseI enzyme reaction solution (NEB, M0303L) were added and incubated at 37°C for 20 min ...