Labshake search
Citations for New England Biolabs :
1 - 50 of 10000+ citations for Palladium PdX API Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... WES and RNA sequencing from the PDX samples was performed using NEBNext Ultra II FS DNA library Kit for Illumina (New England Biolabs), SureSelectXT HS Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library For Illumina Multiplexed Sequencing Platforms (Agilent) ...
-
bioRxiv - Bioengineering 2022Quote: ... 120 nM fluorescent beacon and 1 U.μL-1 murine RNase Inhibitor (NEB M0314) in the reaction buffer containing 50 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Bioengineering 2020Quote: ... using fluorescent CoA 647 (NEB) as substrate ...
-
bioRxiv - Biophysics 2022Quote: ... Non-fluorescent RNA was transcribed using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S). Following in vitro transcription by either kit ...
-
bioRxiv - Biophysics 2023Quote: ... Following mutagenesis for fluorescent labeling and/or creating RTT mutations using the Q5 mutagenesis kit (New England BioLabs), plasmids were transformed into E ...
-
bioRxiv - Cell Biology 2024Quote: ... Fluorescent cell permeable Snap-Cell substrates (NEB) were used in the following concentrations ...
-
bioRxiv - Bioengineering 2020Quote: ... we included LAMP Fluorescent Dye (New England Biolabs) as a general LAMP indicator ...
-
bioRxiv - Cell Biology 2019Quote: A construct expressing SURF4 and the Katushka2S fluorescent marker (PGK-SURF4-p2A-Katushka2S) was assembled with the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequence derived from LV1-5 (Addgene #68411 ...
-
bioRxiv - Molecular Biology 2023Quote: ... by replacing the green fluorescent protein (GFP) with a mCherry reporter using the NEBuilding HiFi DNA Assembly Cloning Kit (New England Biolabs) (Ran et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.3 µM fluorescent SNAP substrate (TMR Star, NEB, S9105) was added along with the primary antibodies
-
bioRxiv - Cell Biology 2022Quote: INS-1 832/3 EV and g1-2 cells transiently expressing the SNAP-GLP-1R were labelled at 37°C with 1 μM of SNAP-Surface 649 fluorescent substrate (S9159S, New England Biolabs) in full media prior to treatment with 100 nM exendin-4 or vehicle for 3 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... the commercially available fluorescent ligand SNAP-Cell TMR-Star (New England Biolabs) was used.
-
bioRxiv - Biophysics 2020Quote: ... The fluorescent probe SNAP-Surface Alexa Fluor 546 (#S9132S) was from NEB Biotech ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were quenched with a non-fluorescent bromothenylpteridine (BTP; New England Biolabs) at 2 µM final concentration ...
-
bioRxiv - Genomics 2021Quote: ... into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB), 2 U M.CviPI (NEB) ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.5 μL of SYTO-9 dsDNA dye (NEB BioLabs, for LAMP fluorescent curve) was used to monitor the real-time amplification signals ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.5 μL of SYTO-9 dsDNA dye (NEB BioLabs, for LAMP fluorescent curve) was used to monitor the real-time amplification signals ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 μM Illumina barcode (NEB-kit) to 23 μl of cDNA ...
-
bioRxiv - Synthetic Biology 2023Quote: ... containing oligo pool template at 3.33 pg/μL and LAMP Fluorescent Dye (B1700, NEB) at 0.5X was aliquoted across a PCR plate ...
-
bioRxiv - Developmental Biology 2019Quote: ... E7.5) into 96 well PCR plates containing 2.5μl of methylase reaction buffer (1× M.CviPI Reaction buffer (NEB), 2U M.CviPI (NEB) ...
-
bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (EV Table 1 ...
-
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... PCR was carried out in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (Forward 5′-TAA TCA GAC AAG GAA CTG ATT A-3′ ...
-
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... were amplified in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler ...
-
bioRxiv - Microbiology 2021Quote: ... PCR was carried out in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 NP-specific primers (Forward 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ ...
-
bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2023Quote: mRNA was isolated from 6-well plates showing a cell confluency of ∼90% using the Monarch Total RNA Miniprep kit (NEB). siRNA transfection was performed 48 hours prior to RNA isolation using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for 1 hr at 72° ...
-
bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for one hour at 72°C ...
-
bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for one hour at 72°C ...
-
bioRxiv - Microbiology 2020Quote: ... with a 384 well plate using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005L, New England Biolabs Inc) and a reaction volume of 10 μl with 2.5 μl of sample ...
-
bioRxiv - Microbiology 2020Quote: ... with a 384 well plate using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005L, New England Biolabs Inc) and a reaction volume of 10 μl with 2.5 μl of sample ...
-
bioRxiv - Cell Biology 2023Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology)6 sequence in-frame with the KRT5 sequence separated by a short peptide linker (DPAFLY) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 uL BsmbI-v2 Golden Gate Assembly Kit (NEB E1602L), and 15.03 uL nuclease free H2O ...
-
bioRxiv - Genomics 2020Quote: ... Capture plate wells contained 5 µl of capture solution (1:500 Phusion High-Fidelity Reaction Buffer, New England Biolabs; 1:250 RnaseOUT Ribonuclease Inhibitor ...
-
bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq DNA Polymerase (NEB, #M0267) for one hr ...
-
bioRxiv - Microbiology 2023Quote: ... Desialylation of Calu-3 cells was achieved by incubating cells grown in a 96 well plate or 24-well plate or 6 well plate with 100 U/mL α2-3,6,8,9 neuraminidase A (P0722L, NEB) in 10% FCS media for 3 h at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... 3 µL of RNA from 4 x 96 well plates was transferred to a 384 well plate with 3 µL of master mix containing 1 µL of a 10 mM stock of dNTPs (NEB Catalog# N0447L ...
-
bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
-
bioRxiv - Neuroscience 2023Quote: ... column purified and ligated at a 1:1 vector to insert ratio using the QuickLig Kit (NEB #M2200S). The ASCL1 stop codon was subsequently mutated using the QuickChange II-XL site-directed mutagenesis kit (Agilent #200523 ...
-
bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed using synthesised cDNA ...
-
bioRxiv - Biochemistry 2022Quote: ... EvaGreen DNA dye was replaced with RPA fluorescent probes (DENV2-CAP-probe at 0.12 μM) with 100 U Exonuclease III (NEB), keeping all other reagents same ...
-
bioRxiv - Cell Biology 2021Quote: ... were performed by confocal microscopy in cells previously labelled with the indicated SNAP-Surface fluorescent probe (New England Biolabs) using a Zeiss LSM-780 microscope with a 63X / 1.4 NA oil objective from the FILM Facility ...
-
bioRxiv - Cell Biology 2019Quote: The fluorescent SNAP-tag substrate BG-CF640R was synthesized by amidization reaction of BG-NH2 (New England Biolabs #S9148S) and CF640R-NHS ester (Biotium #92108) ...
-
bioRxiv - Cell Biology 2022Quote: ... samples were pooled by plate and ExonucleaseI (NEB) digestion was performed ...
-
bioRxiv - Immunology 2023Quote: All PD-1 mutants were generated using Q5 site-directed mutagenesis kit (NEB) following the manufacture’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA was first circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
-
bioRxiv - Developmental Biology 2020Quote: ... We first joined sequences for fluorescent protein mCerulean (CFP) and 2A peptide P2A (a ribosomal skip sequence) with Q5 (NEB) fusion PCR and added them to the pWPXL vector with Gibson Assembly Master Mix (NEB) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The SNAP-tagged histones neosynthesized during the chase time were pulse-labeled by incubating cells with 2 μM of red-fluorescent SNAP reagent (SNAPcell TMR star, New England Biolabs) for 15 min at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 μM of the non-fluorescent SNAP reagent (SNAPcell Block, New England Biolabs) for 30 min at 37°C followed by a 30 min wash in fresh medium and a 2 h chase ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were grown on glass coverslips and pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 µM of the non-fluorescent substrate SNAP-cell Block (New England Biolabs) for 30 min followed by a 30 min-wash in fresh medium and a 2 hr-chase ...