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Citations for New England Biolabs :
1 - 50 of 3935 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 1:50 (NEB, 14001), (5 ...
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bioRxiv - Plant Biology 2022Quote: ... the plasmids were digested with restriction endonuclease PI-PspI (NEB), and the fused EGFP expression cassettes from the digested plasmids were integrated into the pRCS2-ocs-nptII vector for transformation (Chung et al ...
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bioRxiv - Genomics 2023Quote: ... Nuclei were then digested with DpnII (1:50) (NEB, 50 000 U/ml) for 4h at 37°C under agitation ...
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bioRxiv - Genomics 2024Quote: ... samples were digested by incubation in reverse-crosslinking buffer (50 mM Tris pH 8.0, 50 mM NaCl and 0.2% SDS) with 1:50 proteinase K (NEB P8107S) for 30 min at 55 °C in the dark ...
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bioRxiv - Developmental Biology 2020Quote: ... 5μg of the modified BACs was linearized with PI-SceI (NEB) and electroporated into 3×106 mESCs ...
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bioRxiv - Genomics 2022Quote: Samples were digested by incubation in reverse-crosslinking buffer (50 mM Tris pH 8, 50 mM NaCl, 0.2% SDS) with 1:50 proteinase K (NEB P8107S) for 8-16 hours at 55°C ...
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bioRxiv - Molecular Biology 2023Quote: ... MA) in 50 µl 1× CutSmart buffer (NEB), 120 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 pg of lhx1a:DsRed/pI-SceI plasmid DNA was injected into 1-cell stage embryos along with the I-SceI restriction enzyme (NEB, R0694S). These injected embryos were raised to adulthood and screened for DsRed expression in known lhx1a expression domains and isolated a stable Tg(lhx1a:DsRed ...
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bioRxiv - Developmental Biology 2023Quote: ... 5µg of each construct were linearized using PI-SceI (New England Biolabs R0696S) and electroporated into 3×106 mESCs ...
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bioRxiv - Microbiology 2022Quote: ... 10 min) and resuspended in 50 µl of 50 mM Tris-HCl containing 1 µg trypsin (NEB). After overnight incubation at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions (50 µl) containing 1 × Q5 reaction buffer (NEB), 200 µM dNTPs ...
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bioRxiv - Microbiology 2024Quote: ... 50 µM ATP and 1 x RNA ligation buffer (NEB) at 25 °C for 16 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The annealed oligos were diluted 1:50 and then ligated into 50 ng NotI+BamHI-digested pBRα or pACλCI using T4 ligase (NEB), generating pS197 and pS198 ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 µl T4 RNA ligase 1 (NEB, catalog no. M0204). Bead complexes were then washed 3 times with 1 mL PNK buffer ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were incubated with 1:50 Endonuclease V (New England BioLabs) in a calcium containing blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... primers that contained restriction sites for BamHI and XmaI or XbaI (Table S5, primers No. 9-16 and 95-98) and the high-fidelity polymerase Q5 (New England Biolabs) according to vendor’s manual ...
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bioRxiv - Cancer Biology 2024Quote: ... was PCR amplified (14 cycles: 10sec 98°C, 30 sec 57°C, 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...
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bioRxiv - Genetics 2022Quote: ... was digested for 1 h with 50 units of DpnI (New England Biolabs) to eliminate unreplicated plasmids ...
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bioRxiv - Genomics 2024Quote: ... 1 μL T4-BGT (10U/μL) in a 50 μL reaction (NEB #0357) and incubated at 37°C for 1 hour ...
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bioRxiv - Synthetic Biology 2024Quote: ... PI promoters were cloned from the tomato genomic DNA using the Phusion DNA polymerase (New England Biolabs, USA) with addition of overhangs and the BsaI restriction enzyme site for downstream reactions ...
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bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) in 200 μl 1x CutSmart buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) then rinsed with 1xTE.
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bioRxiv - Biochemistry 2021Quote: ... 50 μL PCR products were incubated with 1 μL DpnI enzyme (New England Biolabs) to clean up templates at 37 °C for 2.5 hours and then purified by gel-extraction following the manufacturer instructions (Cat# D2500-02 ...
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bioRxiv - Biochemistry 2021Quote: ... reaction products were resuspended in 50 µl relevant manufacturer’s enzyme buffer (1 × Cutsmart (NEB) for nicking with Nt.BspQI or 1 × Buffer R (ThermoFisher ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, 1 h 50° C) into backbones previously digested with corresponding restriction enzymes (NEB ...
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bioRxiv - Immunology 2024Quote: ... 1 µl per 50 µl pool volume of Thermolabile Exonuclease I (New England Biolabs) was then added and incubated for 4 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... were added in a total of 50 µl 1× CutSmart buffer (New England Biolabs). The reaction was run for 15 min at 37 °C on a thermocycler ...
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bioRxiv - Genomics 2022Quote: To digest linear DNA 1 μg of DNA sample was incubated in 50 μl with 1× NEBuffer 4 (NEB), 1mM ATP (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in 48 μL elution buffer (50 mM Tris pH 8, 1 mM EDTA, 1% SDS) and 1.6 U Proteinase K (NEB), incubated at 55 °C for one hour and then 65 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... in a 10 μL reaction in buffer (50 mM Tris-HCl pH 7.5, 1 mM DTT, 1 U/μL RNase Inhibitor (NEB)) and incubated for 1 hour at 37°C ...
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bioRxiv - Genomics 2023Quote: ... and incubated at 37°C for 1 hour with RNase A/T1 (Thermo, 1/20 volume) and RNase H (NEB, 1/50 volume). The DNA was then purified again.
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bioRxiv - Genetics 2021Quote: ... 1 ul of 20 μM Cas protein (SpyCas9 and SauCas9 from NEB; Nme2Cas9 was purified as previously described (Edraki et al ...
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bioRxiv - Microbiology 2024Quote: ... A 20 µL Gibson Assembly reaction was performed with 50 ng of vector at a 2:1 ratio for 1 hour (New England Biolabs NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... 50 nM Cas9 (NEB), 50 nM synthesized tracrRNA (IDT ...
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bioRxiv - Microbiology 2024Quote: ... and 50% PEG8000 (NEB) to allow for 3’ linker ligation at room temperature for 1.5 hours ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... top and bottom oligonucleotides for a single R-S-R or dead end oligonucleotide pairs were combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using T4 Polynucleotide Kinase (NEB M0201L) for 2 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Insert and vector were combined at a 5:1 ratio and held at 50 °C for 1 hour in NEBuilder® HiFi DNA Assembly Master Mix (NEB). Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen) ...
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bioRxiv - Biophysics 2024Quote: ... the flow cell was rinsed and flushed with 50 µl of 1 mg/ml BSA (NEB) to prevent non-specific binding of the sample.
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bioRxiv - Cell Biology 2023Quote: ... 250 μL of 2 mg mL-1 Y289L GalT,46 50 μL of 500 kU mL-1 PNGase F (New England Biolabs, 5 U μg-1 of protein), and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: ... and murine lung slices were incubated overnight with primary antibodies in 0.1% BSA as follows: anti-CDH1 (clone EP700Y, Biozol, 1:250) or anti-CDH1-Alexa-488 (clone 24E10, New England Biolabs, 1:50), anti-Twist1 (clone Twist2C1a ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 µg/mL BSA(NEB)) in a 50 ml centrifuge tube ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 mU T4 PNK (NEB) and 10 mM RNaseOUT (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... and 50 U Klenow (NEB), for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 nL PEG8000 (50%, NEB) 0.5 nL BSA (20ng/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 nL PEG8000 (50%, NEB), 1.2 nL BSA 20 ng/ml (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 nL PEG8000 (50%, NEB), 1 nL BSA (20 ng/ml NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... 4µl 50% PEG 8000 (NEB), 0.1µl 10mM ATP ...