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Citations for New England Biolabs :
51 - 100 of 3084 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 0.5 mL of rCutSmart™ Buffer (New England BioLabs) containing 0.3% SDS ...
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bioRxiv - Genomics 2022Quote: ... Cells were fixed with 3% paraformaldehyde in PBS for 10 min at room temperature and permeabilized for 5 min on ice in PBS containing 0.5%Triton X-100 and 2mM Vanadylribonucleoside complex (New England Biolabs). Coverslips were preserved in 70% EtOH at -20°C ...
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bioRxiv - Cancer Biology 2019Quote: ... and then resuspended in sorting buffer (PBS with 0.5% BSA, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 µg/mL DAPI and 100 U/mL DNaseI, NEB) and incubated for 30 minutes at room temperature before sorting ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.5% (vol/vol) Triton X-100 in nuclease-free water and 1% (vol/vol) proteinase K (New England Biolabs, P8107S). The sample was digested in this buffer for ≥36h in a humidified ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 20 µl of 0.5 mg/ml RNase A (NEB) and incubation at 37°C for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... and mixed with an equal volume of 2 x RNA loading dye (NEB, B0363S). 25 μl of the mixture was loaded on a 10% TBE-Urea gel (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3.13 μL BSA 2 mg/mL (NEB), 12.5 μL FastDigest NotI (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3.13 μL BSA 2 mg/mL (NEB), 12.5 μL FastDigest NotI (ThermoFisher) ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 μL BSA (NEB, 20 mg/mL) and 400 units of DpnII (R0543M ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mg/ml BSA (New England Biolabs), 10 mM Tris-HCl pH 8 ...
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bioRxiv - Genetics 2021Quote: ... 0.5 μM MPRA_v3_F and MPRA_v3_20I_R primers (Supplementary Table 14) and 2 ng BSA (NEB, B9000). PCR master mix was emulsified by vortexing with 220 μL Tegosoft DEC (Evonik) ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was passed twice at a flow rate of 0.5 mL/min through a column with 6 mL chitin beads (NEB) equilibrated with lysis buffer ...
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bioRxiv - Genomics 2020Quote: ... 2 μl Antarctic Phosphatase [5k U/mL] (NEB) and 1 μl RNase inhibitor and incubating at 37 °C for 30 minutes with shaking ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.5% SDS and 0.5 µg/µL Proteinase K (NEB). The flies were then incubated at 55°C for 2 hrs ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 μL of molecular biology grade bovine serum albumin (20 mg/mL, New England Biolabs), 5 μL of 5× Phire Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% SDS) + 10 μL of 10 mg/mL Proteinase K (New England Biolabs, Cat # P8107S). DIP digestion buffer was added to input samples up to 200 μL ...
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bioRxiv - Cell Biology 2021Quote: ... 50 mM Tris-Cl pH 7.4, 12 mM MgCl2, 1% NP-40, 0.5 mM DTT, 0.1 mg/ml cycloheximide, 80 U/ml NEB murine RNase inhibitor). Beads were transferred to a fresh tube and immunoprecipitated RNA extracted by incubating beads in buffer RLT (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 5 ml 1x NEB Buffer 2 (NEB) and drop frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... packed with 2 ml amylose resin (New England Biolabs) equilibrated with buffer AF ...
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bioRxiv - Genomics 2022Quote: ... 0.7 µl T4 ligase (2 M U/ml, NEB), 250 ng DNA (adapters in File S1 ...
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bioRxiv - Genomics 2022Quote: ... and 2 mg/mL proteinase K (New England Biolabs). The tissue sections were lysed separately in 75 μL lysis buffer for 2 hours at 55 °C ...
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bioRxiv - Microbiology 2023Quote: ... 20 pmoles of dephosphorylated RNA was combined with 2 μl 10 x T4 Polynucleotide Kinase buffer (NEB), 3 μl ATP(y-32P ...
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bioRxiv - Microbiology 2021Quote: ... After digestion the plasmid vector and insert were added to Gibson assembly master mix (1.5 µl insert, 0.5 µl vector, 2 µl master mix) (New England BioLabs) and incubated at 50 °C for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... proteins were pre-treated with 0.5 mM ATP and 2.5 U casein kinase 2 (CK2) in 1X CK2 buffer (NEB) for 15 min at 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of 2 mM dNTP and 0.5 μL (2.5 U) of DNA polymerase I Klenow fragment (New England Biolabs) were added to the reaction mixture ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM NaCl, 2 mM EDTA, 1% NP40, 0.1%SDS, 0.5 mM DTT, 40 U RNase inhibitor [New England Biolabs, cat#M0314L] ...
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bioRxiv - Biochemistry 2020Quote: ... 2 µl T4 DNA ligase buffer and 2 µl 10 mg/ml BSA (New England Biolabs). These reactions were placed in a thermocycler with following program ...
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bioRxiv - Genomics 2019Quote: ... 2 μl of proteinase k (800 units ml-1, NEB) were added and reacted for 1 hour at 50°C ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of 20 mg/ml proteinase K (NEB, P8107S) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were flowed over 2 mL amylose resin (NEB) equilibrated with MBP lysis buffer to capture MBP-tagged protein ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 0.62 µL of 2 mg/mL BSA (NEB #B9001S) with the following thermal cycle condition ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 U/mL DNase I (New England Biolabs, M0303L). Cells were passed through a 40 µm mash and seeded at high density in BME.
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bioRxiv - Microbiology 2023Quote: ... and was loaded onto 2 mL of chitin resin (NEB) pre-equilibrated with lysis buffer ...
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bioRxiv - Systems Biology 2020Quote: ... The purified transposed DNA was amplified with NEBNext High-Fidelity 2 X PCR Master Mix (New England Biolabs) and custom-designed primers with barcodes.30 Gel electrophoresis was used to remove primer dimers from the PCR products with 2% E-Gel EX Agarose Gels (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Final amplification PCR was performed as described before by adding 8 µl of 2 x PCR MM (NEB) with number of cycles according to qPCR (usually Cq values were around 20) ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragments were assembled using the NEBuilder® 2 X HiFi DNA assembly master mix (NEB, Cat. No. #E2621). The resulting plasmid will be referred to as pBd-phoD-HA-BSD.
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bioRxiv - Molecular Biology 2023Quote: ... Fragments were assembled using the NEBuilder® 2 X HiFi DNA assembly master mix (NEB, Cat. No. #E2621). The homology and guide RNA were inserted as described for pBdEF-Cas9-BSD-phodR ...
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bioRxiv - Bioengineering 2023Quote: All qPCR reactions were set up using 7.5 µl of 2 x Luna Universal qPCR master mix (NEB), 0.375 pmol forward primer ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5 µL DNA was incubated (14 h, 37°C) with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix ...
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bioRxiv - Microbiology 2023Quote: ... Annealing was performed with 4 µM (final) of each guide oligo and 0.5-2 µg of template DNA in 1x Cutsmart buffer (NEB) using a slow temperature gradient (95°C – 60°C at 0.1°C / sec ...
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bioRxiv - Cell Biology 2023Quote: Protein G magnetic beads were washed 2-3x with 1ml blocking buffer (20 mM Hepes, 150 mM KCl, 20% Glycerol, 0.5 % Tween, BSA (Biolabs), Heparin 0.2 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Nuclei were pelleted at 500 × g at 4°C for 5 minutes and resuspended in 0.5 mL of 1.2x NEBuffer r2.1 (New England Biolabs) containing 3% sodium dodecyl sulfate (SDS ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 µl DpnI (NEB) was added to each reaction and incubated for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... 0.5 mM dNTPs (NEB), 20 U RNaseOUT (Invitrogen) ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 μl BsaI (NEB), and 0.5 μl T4 DNA ligase ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 mM dNTPs (NEB), 20 U RNaseOUT (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... 0.5 μL MnlI (NEB) was added to the sample ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µl 10 x dNTPs with dUTP instead of dTTP (2 mM each) and 1 µl T4 polymerase (NEB) were added and the mixture was incubated at 37°C in a thermal cycler for 20 min ...
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bioRxiv - Genomics 2019Quote: ... and resuspended in 198 µL A-tailing solution (1× NEB buffer 2, 500 mM dATP, 1% Triton X-100). DNA ends were A-tailed by adding 1.5 µL Klenow (exo- ...