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Citations for New England Biolabs :
201 - 250 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs) following the manufacturer’s protocols.
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bioRxiv - Microbiology 2024Quote: ... The PCR product of the selected deletion mutant was purified using Monarch PCR and DNA cleanup kit (NEB) and sent for sequencing at Eurofins Genomics LLC (Louisville ...
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bioRxiv - Molecular Biology 2020Quote: ... Genotyping was performed by amplifying a 619bp region around the cutting sites looking for acquired resistance to EcoRI (NEB) cutting and or bigger indels ...
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bioRxiv - Genomics 2021Quote: ... DNA was purified using Monarch® PCR & DNA Cleanup Kit (NEB), using the following recommended protocol modification ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was purified using the Monarch PCR & DNA Cleanup Kit (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and purified using Monarch PCR clean-up kit (New England Biolabs). Blunted DNA was A-tailed using NEBNext dA-tailing kit and column-purified ...
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bioRxiv - Biochemistry 2020Quote: ... purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs, USA) and cloned into PET-24a (+ ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR product was transcribed using the T7 IVT Kit (NEB), followed by DNase I (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB, T1030L). The source of the 3xHA-Nluc starting CDS (“Nluc start” ...
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bioRxiv - Synthetic Biology 2021Quote: ... followed by purification with Monarch® PCR & DNA Cleanup Kit (NEB). The Mobius Assemblies were verified first by colony PCR using GoTaq® Green Master Mix (Promega ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were purified with Exo-CIP Rapid PCR Cleanup Kit (NEB) following supplier’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... or the Exo-Cip Rapid PCR Cleanup Kit (New England Biolabs). Gene editing efficiency was analyzed using TIDE analysis software (Brinkman et al ...
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bioRxiv - Microbiology 2021Quote: ... cleaned up using the Monarch PCR Purification kit (New England Biolabs), and used in a Gibson assembly reaction (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: PCR amplicons and plasmids were purified using Monarch DNA kits (NEB). PCR ...
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bioRxiv - Genomics 2021Quote: ... and DNA cleaned with Monarch PCR & DNA Cleanup Kit (NEB T1030S). Resulting supercoiled plasmid is linearized at the 3’ end of the PolyA tail using BamHI-HF (NEB R3136S) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were quenched with Monarch PCR & DNA Cleanup Kit (NEB T1030S) and the purified DNA eluted in 20 µL EB buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Assembled constructs were purified using Monarch PCR purification kit (NEB, T1030S). The assembled plasmid library was electroporated into electrocompetent bacteria (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Assembled constructs were purified using Monarch PCR purification kit (NEB, T1030S) and used to electroporate into electrocompetent bacteria (NEB ...
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bioRxiv - Microbiology 2022Quote: ... PCR products and plasmids were purified with Monarch DNA kits (NEB). Overlapping primers for amplification were designed using the NEBuilder assembly tool (https://nebuilderv1.neb.com/ ...
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bioRxiv - Microbiology 2023Quote: ... and Luna Universal one-step qRT-PCR kit (New England BioLabs) as previously described (13 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the kit Phusion High-Fidelity PCR (New England, Biolabs), containing Phusion Buffer 1X ...
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bioRxiv - Genetics 2023Quote: ... amplicons were purified using Monarch® PCR & DNA Cleanup Kit (NEB).
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted with the Monarch PCR & DNA Cleanup kit (NEB). Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... PCRs were undertaken using the Q5 High-Fidelity Polymerase kit (NEB) and thermocycling conditions of 98°C ...
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bioRxiv - Neuroscience 2023Quote: ... and Sanger sequenced using the NEB PCR Cloning Kit (NEB, #E1202S).
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bioRxiv - Systems Biology 2024Quote: ... followed by purification using a Monarch PCR&DNA Cleanup Kit (NEB). Ring-ligation was carried out using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using a post-PCR cleanup kit (New England Biolabs) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Polymerase chain reaction (PCR) products and plasmids were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product was purified using Monarch PCR and DNA Cleanup kit (New England Biolabs, Inc., Ipswich, MA, USA) and DNA concentration was quantified using NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We performed a 10-cycle PCR using a Phusion PCR Kit according to the manufacturer’s protocol (New England Biolabs) with multiplexed primers and adjusted PCR products to 10 μM for sequencing on either an Illumina HiSeq2500 (125 bp paired-end ...
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bioRxiv - Microbiology 2020Quote: ... all PCR products were purified using the Monarch® PCR and DNA Clean-up Kit (New England Biolabs, USA) following the manufacturer’s instructions and eluted in molecular grade water.
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bioRxiv - Microbiology 2021Quote: ... The entire ORF of mucoricin was PCR amplified from cDNA using Phusion High-Fidelity PCR Kit (New England Biolabs) using the primers 5’-GATAAGACTAGTATGTATTTCGAAGAAGGC-3’ and 5’-GGTGATGCACGTGTCCTTCAAATGGCACTA-3’ ...
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bioRxiv - Bioengineering 2022Quote: ... the 5S and Saba PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB, # T1030). The purified DNA sequence was then analyzed through Sanger sequencing (GENEWIZ from Azenta ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and successful PCR products were cleaned up using Monarch® PCR and DNA Clean-Up Kit (New England Biolabs) as per kit instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Evolutionary Biology 2023Quote: ... beads were directly used for off-bead PCR amplification using the Phusion High-Fidelity PCR kit (New England Biolabs) for 30 cycles with primers from library amplification (Rd1 ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were then either purified directly using Monarch PCR and DNA Cleanup Kit (New England Biolabs, Hitchin, UK), or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicon was subsequently purified with column DNA Clean-up kit (Monarch PCR & DNA Cleanup Kit, # T1030L, NEB) and mixed with double-digested Nhe1/ BamH1 and gel-purified target pCW57.1 vector in a molecular ratio of 1:2 (50 ng Vector ...
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bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction (PCR) was performed on a genomic rat DNA template using a Taq PCR Kit (New England Biolabs), and the subsequent PCR product was purified using a Qiagen PCR Purification Kit (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... Libraries were quantified using a Qubit fluorometer before an 18-cycle PCR amplification on bar-coded fragments with Phusion high-fidelity PCR Kit (New England Biolabs). The PCR products were cleaned using 1X vol of AMPure XP Beads.
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bioRxiv - Microbiology 2019Quote: ... The PCR products were cleaned up using the Monarch® PCR and DNA clean-up kit (New England Biolabs, USA). The PCR samples were mixed with 125 μl of the DNA Clean-Up Binding Buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... Genetic parts were amplified using primers listed in Table S1 and PCR products were purified with the Monarch PCR & DNA Clean up kit (NEB). Parts were assembled into plasmid constructs mainly by Gibson assembly [41] using the isothermal method ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses were performed using Luna Universal Probe One-Step qRT-PCR Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... these long gene sequences containing enzyme sites firstly was amplified by common PCR by Q5 high-fidelity PCR kit (Cat: #M0491, NEB), using primer F and R pairs of S or N ...
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bioRxiv - Microbiology 2020Quote: DNA probes were synthesized via PCR with Q5 high-fidelity polymerase (Thermo) and purified with Monarch PCR Cleanup Kit (NEB). 75ng probe A/probe B and 150ng probe C/probe D were added to increasing concentrations of protein (4% ...
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bioRxiv - Genomics 2022Quote: Real-time PCR (RT-PCR) amplification of RNA was performed following the Luna Universal One-Step RT-qPCR kit (New England Biolabs). A CFX 96 Touch Real-Time PCR Thermocycler/ Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... an 887bp portion of mtDNA containing nd-1 was amplified by PCR and TA-cloned into pMiniT2.0 using the NEB PCR cloning kit (NEB E1202S). Purified plasmid was linearized with BamHI-HF (NEB 3136) ...