Labshake search
Citations for New England Biolabs :
1 - 50 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... all kinases were tested for activity using 2 µg each of histone H1 (NEB) or Myelin Basic Protein (MBP ...
-
bioRxiv - Molecular Biology 2023Quote: ... Editing activity of α-synCas was evaluated using EnGen Mutation Detection Kit (NEB). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
-
bioRxiv - Systems Biology 2024Quote: ... the enzyme’s activity was immediately neutralised using the Monarch PCR & DNA Cleanup Kit (NEB). Subsequently ...
-
bioRxiv - Immunology 2021Quote: We quantified plasma luciferase activity by a BioLux Gaussia Luciferase Assay Kit (NEB, Ipswich, MA) from 10 μL plasma acquired by retro-orbital bleeding ...
-
bioRxiv - Synthetic Biology 2020Quote: ... GLuc activity in samples was measured using the Biolux Gaussia Luciferase Assay kit (NEB, Ipswich, MA). GLuc assay solution (50 μL ...
-
bioRxiv - Biochemistry 2021Quote: ... The subsequent CRISPR detection was performed by adding 8.75 μL of CRISPR Mix (1X TtCmr Activity Assay Buffer, ~62.5 nM TtCmr complex, 500 nM TTHB144, 2 μL NTP Buffer Mix (NEB #E2050), 25 U Hi-T7 RNA polymerase (NEB #M0658) ...
-
bioRxiv - Cancer Biology 2024Quote: The dePARylation activity of PARG was measured in the PARP1 Histone H4 Activity Assay (#K611, Tulip BioLabs). Briefly ...
-
bioRxiv - Neuroscience 2019Quote: ... and 24 hours of incubation GLuc activity was quantified using the BioLux Gaussia Luciferase Assay Kit (NEB) according to the manufacturer’s instructions with luminescent detection on a SpectraMax M5 Microplate Reader (Molecular Devices ...
-
bioRxiv - Molecular Biology 2023Quote: Internally fluorescein-labelled RNA was produced by ligation of in vitro transcribed 5′ acceptor RNA fragment with chemically produced 3′ donor RNA containing internal fluorescein dT modification and 5′ monophosphate essential for ligase activity using T4 Ligase 2 (NEB #M0239S). Additionally ...
-
bioRxiv - Genomics 2024Quote: ... Large (Klenow) fragment without exonuclease activity (exo-) (NEB) in combination with dATP or dGTP ...
-
bioRxiv - Immunology 2022Quote: ... cleavage rates were transformed into standardized catalytic activities (1 “eqU” = equivalent to the catalytic activity of 1 U RNase HII (NEB ®) under standard conditions) ...
-
bioRxiv - Systems Biology 2020Quote: ... We neutralized the tagmentation activity of Enzyme 1 by immediately cleaning the reaction with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... Secreted cypridina Luciferase (cLuc) activity from the internal control plasmid was similarly measured using the cypridina Luciferase kit (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: Pacy-2::acy-2 genomic was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
-
bioRxiv - Neuroscience 2019Quote: ... Editing activity was validated by the T7E1 assay (NEB) in rat C6 glioma cells (ATCC) ...
-
bioRxiv - Neuroscience 2023Quote: ... used in T7 endonuclease I activity assays (NEB E3321) as previously described (Baker et al. ...
-
bioRxiv - Cancer Biology 2023Quote: ... MNase activity was stopped and chromatin fragments released into supernatant were purified using the NEB Monarch DNA Cleanup Kit (NEB, T1030) per manufacturer’s instruction ...
-
Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 µM HDVLig (pre-adenylated using a 5’ adenylation kit (NEB)) ...
-
bioRxiv - Genetics 2021Quote: In vitro methylation reactions assaying methyltransferase activity of dam or HpaII (NEB) on DNA were performed in the buffer supplied with the commercial recombinant enzyme (New England Biolabs (NEB ...
-
bioRxiv - Microbiology 2019Quote: ... (2) DSBs blunting with NEB’s Quick Blunting Kit (NEB, cat. number: E1201); (3 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 µM IllLigBio adapter pre-adenylated using a 5’ adenylation kit (NEB), 20-200 nM pppUGAAUG hexamer standard ...
-
bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
-
bioRxiv - Microbiology 2022Quote: ... human NINL isoform 2 (NCBI accession NM_001318226.2) and the NINL isoform 2 mutant (Q231R) were mutagenized using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The plasmids encoding 3C proteases (coxsackievirus B3 (CVB3 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and ligated to hairpin adaptor 2 (ENDseq-adaptor-2, 5′-Phos-GATCGGAAGAGCACACGTCUUUUUUUUAGACGTGTGCTCTTCCGATC*T-3′ [*phosphorothioate bond]) (Quick ligation kit, NEB). To prepare libraries for sequencing ...
-
bioRxiv - Immunology 2020Quote: ... All in vitro activity assays with recombinant GST-IdeZ or IdeZ (NEB Catalog #P0770S) (1ug per reaction ...
-
bioRxiv - Microbiology 2021Quote: ... RNase III activity was assayed using a 40mer duplex RNA and dsRNA ladder (NEB).
-
bioRxiv - Microbiology 2020Quote: ... amplified with the primers Dhm1275-1 and −2 (Table 2) using a NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... 2 µL of Taq DNA polymerase (LongAmp Taq DNA Polymerase kit, New England Biolabs), 7.5 µL of dNTPs (dNTP set ...
-
bioRxiv - Developmental Biology 2019Quote: Pceh-23_L::acy-2(genomic) was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
-
bioRxiv - Cell Biology 2020Quote: The pIGLR-2∷mCherry construct was generated with a Gibson assembly cloning kit (NEB) with the following four fragments ...
-
bioRxiv - Molecular Biology 2023Quote: ... or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2: NEB, E7645S), according to the manufacturers’ protocols ...
-
bioRxiv - Biochemistry 2022Quote: ... a buffer recommended by New England Biolabs to ensure optimal enzyme activity (New England Biolabs, n.d.), were added to each well (Table 2) ...
-
bioRxiv - Molecular Biology 2024Quote: ... a Taq polymerase with proof reading activity was used (e.g. Phusion High Fidelity DNA polymerase, NEB). PCR products were separated on a 1–3 % TBE agarose gel.
-
bioRxiv - Biochemistry 2021Quote: ... and 2 μL T7 RNA Polymerase mix (HiScribe T7 High Yield RNA Synthesis Kit, NEB) were incubated at 37°C for 3 h ...
-
bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were prepared using the NEBNext ARTIC SARS-CoV-2 Library Prep Kit (NEB) and ARTIC V3 (MHome ...
-
bioRxiv - Microbiology 2022Quote: ... and library preparation was performed using the NEBNext ARTIC SARS-CoV-2 FS kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
-
bioRxiv - Cell Biology 2022Quote: ... 2 μL GlycoBuffer 2 10X (NEB), 2 μL NP-40 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3’-phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB ...
-
bioRxiv - Biochemistry 2023Quote: ... The peak of activity was pooled and half of the pool was treated with Endo Hf (NEB) by first dialyzing the 25 ml against 2 changes of 500 ml of 20 mM Na acetate pH 6.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... SNAP-tag coupling activity (methodology described below) was first tested with a small ligand BG-biotin (NEB) or DNA (a 550 bp benzylguanylated PCR product ...
-
bioRxiv - Developmental Biology 2023Quote: ... and a subset of F0 embryos were dissociated and used in T7 endonuclease activity assays (NEB E3321) in order to validate successful CRISPR-Cas9 activity ...
-
bioRxiv - Developmental Biology 2019Quote: Pceh-23_L::acy-2 fragment(anti-sense) was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
-
bioRxiv - Microbiology 2022Quote: ... a sequencing library was constructed with the NEBNext ARTIC SARS-CoV-2 FS kit (NEB E7658S). For each viral variant ...
-
bioRxiv - Cancer Biology 2023Quote: ... TrAEL-seq adaptor 2 was added using a modified NEBNext Ultra II DNA kit (NEB E7645S): 3.5 μl NEBNext Ultra II End Prep buffer ...
-
bioRxiv - Molecular Biology 2023Quote: crRNA (Supplementary Table 2) was synthesized using a HiScribe T7 High Yield RNA Synthesis Kit (NEB). The DNA sequences includes the T7 promoter at the 5’ end and the sequence from crRNA with the target sequence at the 3’ end ...
-
bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (EV Table 1 ...
-
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... PCR was carried out in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (Forward 5′-TAA TCA GAC AAG GAA CTG ATT A-3′ ...