Labshake search
Citations for New England Biolabs :
251 - 300 of 3636 citations for Oligodendrocyte transcription factor 1 OLIG1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... PCR amplicons were used as template for in vitro transcription via T7 polymerase (New England BioLabs (NEB)) ...
-
bioRxiv - Biophysics 2022Quote: ... sgRNA was produced by in vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB) with the PCR amplified DNA sequence as the template ...
-
bioRxiv - Biochemistry 2022Quote: ... RNA substrates were produced by run-off in vitro transcription using T7 RNA polymerase (New England Biolabs) and purified via anion exchange chromatography as described before with slight modifications 47,48 ...
-
bioRxiv - Plant Biology 2022Quote: ... RNA transcription in sense and antisense orientations was performed with the T7 RNA polymerase (NEB Biolabs; M0251S) using Digoxigenin-11-UTP (Roche ...
-
bioRxiv - Plant Biology 2022Quote: ... RNA transcription in sense and antisense orientations was performed with the T7 RNA polymerase (NEB Biolabs; M0251S) using Digoxigenin-11-UTP (Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... The transcription of guide RNAs (gRNAs) was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... 400ng of total RNA was used for reverse transcription using LunaScript™ RT SuperMix Kit (NEB, USA). qPCR was performed using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium ...
-
bioRxiv - Microbiology 2022Quote: ... Reverse transcription for first-strand cDNA synthesis was performed by using the LunaScript RT SuperMix Kit (NEB) containing both oligo(dT ...
-
bioRxiv - Genomics 2023Quote: ... Reverse transcription reactions (RT +) were then performed with 5 µg of RNA and random hexamers (NEB, #S1330S), using the Superscript III Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... and subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S), then treated with ExoSAP-IT (Affymetrix ...
-
bioRxiv - Genomics 2023Quote: ... Each reverse transcription reaction contained 8 μL template RNA and 2 μL LunaScript RT SuperMix (NEB #E3010). The reaction condition for reverse transcription was ...
-
bioRxiv - Molecular Biology 2024Quote: ... and used for in-vitro transcription using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Finally ...
-
bioRxiv - Cell Biology 2024Quote: ... Reverse transcription reactions were performed using a LunaScript RT SuperMix Kit (#E3010L, New England Biolabs, Ipswich, MA) with 500 ng of total RNA ...
-
bioRxiv - Immunology 2023Quote: ... in vitro transcription was performed over-night with the T7 High Yield RNA polymerase IVT kit (NEB) for 37°C overnight ...
-
bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... or a polyclonal rabbit antibody against cleaved casp-3 (1:100; New England Biolabs, Frankfurt, Germany), followed by a biotinylated goat anti-rabbit secondary antibody (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunoblots in this study were anti-tau (tau46) (1:5000; NEB 4019S), anti-human tau (tau13 ...
-
bioRxiv - Microbiology 2022Quote: ... Before subjected to the in vitro transcription reaction to synthesize mRNA with T7 RNA polymerase (NEB, MA,USA), the DNA template was linearized with EcoRV (NEB ...
-
bioRxiv - Neuroscience 2020Quote: ... and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing, NEB, E2060S). Transcription products were purified using RNAse-free SPRI beads ...
-
bioRxiv - Bioengineering 2020Quote: ... We performed in vitro transcription using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), and purified the resulting RNA using a GeneJET RNA Purification Kit (Thermo Scientific) ...
-
bioRxiv - Bioengineering 2020Quote: ... In vitro transcription was conducted using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs (NEB)) as previously described (23) ...
-
bioRxiv - Bioengineering 2020Quote: ... In vitro transcription was conducted using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs (NEB)) as previously described (23) ...
-
bioRxiv - Microbiology 2021Quote: ... Reverse transcription and qRT-PCR analysis was performed by Luna universal one- step qRT-PCR kit (NEB # E3005L) with primers list in Table 9.
-
bioRxiv - Developmental Biology 2021Quote: ... and cDNA was generated by reverse transcription using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB). cDNA was amplified with primers flanking each splice site by Taq polymerase (NEB ...
-
bioRxiv - Cancer Biology 2020Quote: ... in vitro transcription with T7 polymerase was done by T7 high yield RNA systhesis kit (New England Biolabs). Purification of mRNA was done by Qiagen mini column ...
-
bioRxiv - Biochemistry 2020Quote: ... The transcription reaction was carried out at 37°C for 12 hours with T7 polymerase (New England Biolabs) in the presence of 13C/15N labeled nucleotide triphosphates (Cambridge Isotope Laboratories ...
-
bioRxiv - Synthetic Biology 2021Quote: ... In vitro transcription was performed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, New England Biolabs). The following reagents were combined ...
-
bioRxiv - Synthetic Biology 2019Quote: ... in vitro transcription was performed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). The mixture contained 10 µL of NTP buffer mix ...
-
bioRxiv - Cancer Biology 2019Quote: ... In vitro transcription was carried out by the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs). Resulting transcriptions products were treated with DNAse I ...
-
bioRxiv - Genetics 2020Quote: ... pyogenes chimeric single guide RNA through in vitro transcription using a HiScribe T7 Quick kit (New England Biolabs). The gRNA was then precipitated in a 1/10 volume of 3M Sodium Acetate and two volumes of 100% ethanol by chilling the reaction at −20 °C for 15 min ...
-
bioRxiv - Immunology 2022Quote: ... in vitro transcription of BNT162b2 mRNA was performed with a HiScribe T7 ARCA mRNA Kit with tailing (NEB) with 2.5 mM N1-Methylpseudouridine-5’-triphosphate nucleoside analog (TriLink BioTechnologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... linearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411 ...
-
bioRxiv - Plant Biology 2022Quote: ... the At5g20040 transcription unit was amplified from Col-0 cDNA using the Q5 High-Fidelity DNA Polymerase (NEB), as recommended by the manufacturer using the oligonucleotides attB_IPT9_F and attB_IPT9_R ...
-
bioRxiv - Cell Biology 2023Quote: ... was added at 5mM concentration to the transcription reaction along with 0.1 U of inorganic pyrophosphatase YIPP (NEB). In vitro transcription was typically performed for 3 h at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... and then transcribed using pooled in vitro transcription (HiScribe T7 High Yield RNA Synthesis kit, New England BioLabs) and cleaned up with the NEB Monarch RNA Cleanup kit ...
-
bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
-
bioRxiv - Developmental Biology 2023Quote: ... Transcription reaction volume was reduced to 10 µl and 0.5 units of thermostable inorganic pyrophosphatase (New England Biolabs) was included in the reaction ...
-
bioRxiv - Genomics 2023Quote: ... The cDNAs were built by reverse transcription of proliferating MuSC mRNAs using M-MuLV Reverse Transcriptase (NEB #M0253L). mRNAs were extracted using NucleoSpin RNA Plus XS kit (Macherey-Nagel #740990.50) ...
-
bioRxiv - Neuroscience 2023Quote: ... and then transcribed using pooled in vitro transcription (HiScribe T7 High Yield RNA Synthesis kit, New England BioLabs) and cleaned up with the NEB Monarch RNA Cleanup kit ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli in vitro transcription were synthesized through a 200 µL PCR reaction containing 10x Taq Buffer (NEB, #B9014S), 250 µM dNTP Mix (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... Templates for in vitro transcription were generated by PCR amplification (Q5 High-Fidelity DNA Polymerase, New England Biolabs) from plasmids using a T7 extended primer (5’-CCTAATACGACTCACTATAGGGAG-3’ 85) ...
-
bioRxiv - Microbiology 2023Quote: ... Reverse transcription was carried out using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs #E6560S) with random hexamer mix ...
-
bioRxiv - Genomics 2024Quote: DNA templates for in vitro transcription of λRNA were generated from a defined region of λDNA (NEB, N3011S) through PCR amplification ...
-
bioRxiv - Microbiology 2024Quote: ... and in vitro transcription and polyadenylation was performed using HiScribe T7 ARCA mRNA Kit (with tailing) from NEB, following recommended procedure ...
-
bioRxiv - Molecular Biology 2024Quote: ... the products were used to generate RNA transcripts using the HiScribe T7 In Vitro Transcription Kit (NEB, E2040S) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized via reverse transcription from ~500 ng of RNA using LunaScript RT Supermix (New England Biolabs). Transcripts of interest were subsequently quantified using Luna qPCR master mix (New England Biolabs ...
-
bioRxiv - Cell Biology 2019Quote: ... then incubated with either mouse anti-MBP antibodies at a dilution of 1:5000 (New England Biolabs) or 1:2500 mouse anti-GFP antibodies (Roche ...
-
bioRxiv - Cell Biology 2020Quote: ... The primary antibodies used in this study were rabbit anti-GLuc 1:2,000 (E8023S, New England Biolabs), rabbit anti-epidermal growth factor receptor (EGFR) ...
-
bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed overnight under rotation at 4 using 1/100 T7RNA antibody (Biolabs CB MAB-0296MC) and antiflag (Sigma F1804 and F3165) ...