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Citations for New England Biolabs :
151 - 200 of 269 citations for Nuclease, Micrococcal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and digested with 2 U/μl of DpnII restriction nuclease (NEB) at 37 °C overnight ...
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bioRxiv - Synthetic Biology 2021Quote: ... SARS-CoV-2 RNA was replaced with nuclease free water (NEB) for the assay without the target RNA ...
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bioRxiv - Zoology 2020Quote: ... with 200μl 0.1U Nuclease Bal 31 enzyme (New England Biolabs #M0213S) at 30°C ...
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bioRxiv - Bioengineering 2022Quote: ... we used 10 uL of nuclease-free water (BioLabs, cat# 52904B1500S) as an elution buffer to obtain the extracted RNAs from the membrane.
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bioRxiv - Genetics 2023Quote: ... diluted to 2 mg/ml in Nuclease-Free Water (NEB, B1500L), at 37°C for 15 minutes each ...
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bioRxiv - Developmental Biology 2023Quote: ... We then performed a T7E1 nuclease assay (M0302, New England Biolabs) according to the manufacturer’s protocol and resolved digested products on a 1.2% agarose gel containing STBYTM safe DNA gel stain (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Optimal digestion by P1 nuclease (100 U/µL, New England Biolabs) required lysate to be adjusted to pH 6.5 before digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... Then gDNA was digested into nucleosides using the nuclease P1 (NEB) and Antarctic Phosphatase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 450 Units of P1 Nuclease (100 U/μL, New England Biolabs) were added per 30 μg of RNA and lysates were incubated at 37 °C for 1 hr with gentle rotation ...
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bioRxiv - Cell Biology 2020Quote: ... was then removed by digestion with Mung Bean Nuclease (New England Biolabs): 1 U of Mung Bean Nuclease was added to 1 μg of digested vector in 30 μl of 1x Cutsmart buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting pellet was resuspended in nuclease free water (New England Biolabs) and RNA quantified through measurement of A260 prior to storage at −80 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 30 nM dCas9 protein (dCas9 Nuclease, S. pyogenes, M0652, New England Biolabs), 1–4× 104 Beads@oligo ...
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bioRxiv - Genomics 2019Quote: ... 758μl nuclease free water and 10μl (4000U) T4 DNA ligase (NEB, M0202M). Ligation was carried out at 16°C for 4h and room temperature for additional 2h ...
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bioRxiv - Biophysics 2020Quote: Circular DNA constructs were nicked using the Nt.BspQI nuclease (New England BioLabs) and purified from 1% agarose gel ...
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bioRxiv - Immunology 2022Quote: ... Eluted RNA was incubated with 180 units of nuclease P1 (NEB, M0660) in nuclease P1 buffer at 37 °C for 90 min ...
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bioRxiv - Microbiology 2022Quote: ... Nuclease digestions using specified restriction enzymes (New England Biolabs [NEB], 20 units) or exonuclease III (NEB ...
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bioRxiv - Microbiology 2022Quote: ... Nuclease digestions using specified restriction enzymes (New England Biolabs [NEB], 20 units) or exonuclease III (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... and nuclease-free H2O up to 20 μL (New England Biolabs, USA). The reaction was performed in 30 cycles of 37 °C for 5 min followed by 16 °C for 5 min and finalized by incubation at 60 °C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... 1μl of 50μM tracer and 1μl Cas9 nuclease (New England Biolabs, MO386M). As a control ...
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bioRxiv - Microbiology 2024Quote: ... samples were split and treated with either 100 U Nuclease P1 (NEB) and 10 U Antarctic phosphatase ...
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bioRxiv - Microbiology 2019Quote: ... DNA restriction nucleases were used to digest plasmids (Promega and New England Biolabs). Gibson assembly mixes were used according to manufacturer instructions to construct the plasmids used in this study (New England Biolabs) ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was resuspended in 10 μl of nuclease free water (New England Biolabs). 5 μl of RNA solution was suspended in 5 μl of RNA loading buffer (95% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... HiFi Cas9 Nuclease V3 (IDT) were mixed with 1X of CutSmart Buffer (NEB) and nuclease-free water to a total of 10 μL then incubated for 30 minutes at room temperature and kept on ice for immediate use.
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bioRxiv - Cancer Biology 2021Quote: ... 5 µl Reverse External Primer (10 µM) and Nuclease-free water (NEB,USA) up to 100 µl were mixed on ice ...
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bioRxiv - Biophysics 2020Quote: ... 1 µl of Universal nuclease (Pierce; 125U) and 10 of DNaseI (NEB; 20U) were added to the sample and incubate for 10 min at RT ...
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bioRxiv - Genomics 2022Quote: ... 25 μl of nuclease free water and 2 μl of DnaseI (M0303S, NEB) were added to degrade the DNA template ...
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bioRxiv - Microbiology 2022Quote: ... using XbaI-linearized and Mung Bean Nuclease-treated (enzymes from New England Biolabs) JFH1-QL/GLuc2A plasmid (42) ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were further processed and digested with P1 Nuclease (Cat#: M0660S, NEB). In vitro-transcribed 4B mRNA (firefly luciferase reporter (Bukhari et al. ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of nuclease-free water and 5 µL of GA mastermix (NEB) were added and incubated at 40°C for a minimum of 1.5 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Single-stranded RNA and DNA were digested with mung bean nuclease (New England BioLabs) and RNase A over 1 h at 37 °C in a buffer solution (50 mM NaOAc pH 5 ...
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bioRxiv - Microbiology 2020Quote: ... to ensure sterility before incubation for 45 minutes with 24 μl microccocal nuclease (NEB) and 15 μl DNaseI (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... dsRNA was treated by enzymatic digestion with DNaseI and S1 nuclease (New England Biolabs) to remove DNA and ssRNA from the samples ...
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bioRxiv - Microbiology 2023Quote: 100 μM of compound was incubated with 0.02 units P1 nuclease (New England Biolabs) in P1 reaction buffer (50 mM sodium acetate pH 5.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK) (NEB, M0201). The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938) ...
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bioRxiv - Genomics 2023Quote: ... as described before. Cas9-gRNA complex (6.6 uM S. pyogenes Cas9 Nuclease (NEB, M0386T), 20 uM gRNA ...
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bioRxiv - Genetics 2020Quote: Oligonucleotide libraries were re-suspended in nuclease-free water and amplified by emulsion PCR (NEB Q5 polymerase ...
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bioRxiv - Plant Biology 2021Quote: ... total or virion DNA was treated with 1 unit of Mung Bean nuclease (NEB, Massachusetts). The digestion products were analyzed for SEGS-2 episomes as described above.
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bioRxiv - Genetics 2020Quote: ... 1µl of 6000nM Streptococcus pyogenes Cas9 Nuclease (New England Biolabs, cat no. M0386; 200nM final), and pre-incubate this for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Then the tRNA were digested with 10 U Nuclease P1 (New England Biolabs, MA, USA) at 37°C overnight followed by dephosphorylation using 10 U bacteria alkaline phosphatase (Thermofisher Scientific ...
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bioRxiv - Genomics 2022Quote: Fungal material was harvested and treated with microccocal nuclease (MNase, cat. #MS0247S, New England BioLabs). For Lmb and Lml ...
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bioRxiv - Molecular Biology 2023Quote: ... the DNA was further treated with 1µL of Mung Bean Nuclease (New England Biolabs #M0250L) for 1 hour at 37ºC ...
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bioRxiv - Immunology 2023Quote: 200 ng extracted mRNA was digested into nucleosides by Nuclease P1 (1 U, NEB, M0660S) and shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin in the cell lysate was digested using Microccocal nuclease (MNase, New England BioLabs, M0247S) at room temperature for 5 min and 5 ul 0.25 mM EDTA was added to stop the reaction ...
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bioRxiv - Genomics 2021Quote: ... The pellet was dissolved in 200 μl of nuclease free water (New England BioLabs, Massachusetts, USA) over 2 days ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (in 10.5 μl of nuclease-free water) were combined with 1 mM ATP (NEB), 40 U RNaseOUT ...
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bioRxiv - Immunology 2019Quote: Transient dual knockdown of egfra and erbb2 was induced using a Cas9 nuclease (New England Biolabs) in combination with transactivating RNA (tracr ...
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bioRxiv - Molecular Biology 2022Quote: ... 5ul of each reaction was digested with P1 nuclease and treated with proteinase K (NEB #P8107S), prior to application to a TLC membrane (Machery-Nagel ...
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bioRxiv - Microbiology 2023Quote: ... Cas9 production was quantified by serial dilution as described using Cas9 nuclease (New Engliand BioLabs M0386S). HFF-hTERT cells were transduced with Nanoblades with 8 μg/mL polybrene (Boston BioProducts BM-862M-1 ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was washed with 80% ethanol and resuspended in 12 μl nuclease free water (NEB). 1 μl of the prepared cDNA library was subjected to Bioanalyzer analysis.
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bioRxiv - Molecular Biology 2024Quote: Total RNA (5 µg in 20 µL ddH2O) was incubated with 2µL 100U Nuclease P1 (NEB) and 2 µL 10x Nuclease P1 buffer (NEB ...