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Citations for New England Biolabs :
151 - 200 of 1434 citations for Nipah Virus Glycoprotein F Recombinant Protein Human Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 2 μL PNGase F (NEB # P0704) was added to the filter and incubated for 3 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... PNGase F (New England Biolabs, #P0704) released N-glycans from 1 mg of brain protein lysate were isolated as previously described44 ...
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bioRxiv - Molecular Biology 2022Quote: ... or iii) 500U PNGase F (NEB) for 1h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and PNGase F (New England Biolabs) (1:100=enzyme ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µl PNGase F (P0708, NEB) was then added ...
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bioRxiv - Microbiology 2023Quote: ... or PNGase F (New England Biolabs) according to the manufacture’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... PNGase F from Elizabethkingia meningoseptica (NEB), acetonitrile (ACN) ...
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bioRxiv - Immunology 2023Quote: ... via recombinant neuraminidase (NEB P0720L) as described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: 100μg of each P13 and P40 sample was resuspended with 20μL of 1X glycoprotein denaturing buffer (New England BioLabs) and incubated at 100°C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and de-N-glycosylated using 500 U N-glycosidase F (PNGase F, New England Biolabs) for 12 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... then deglycosylated with 2000 U of Peptide-N-Glycosidase F (PNGase F) (New England BioLabs, MA, USA). All samples were incubated for 16 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... was inserted into a published oligonucleotide scaffold (Talbot and Amacher, 2014) and injected together with recombinant Cas9 protein (New England Biolabs) into 1-2 cell stage zebrafish (AB strain) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ...
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bioRxiv - Cancer Biology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 µg/well of purified BSA (NEB) in a volume of 50 µl ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... coli C2523 pMAL-c5X vector and recombinant MUP (rMUP) was made using pMAL Protein Fusion and Purification System (New England Biolabs) using methods similar to prior studies27,36 ...
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bioRxiv - Developmental Biology 2019Quote: ... The recombinant DBINO domain containing protein (~80kDa) was purified using the amylose affinity column (New England Biolabs, Massachusetts, United States) and detected using the anti-MBP-HRP antibody (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... The commercially available vaccinia virus capping system (NEB #M2080S) was used as a positive control.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins (except from cells expressing eGFP and cytoplasmically tagged EGFR) were further treated with 250 U of PNGase F (NEB, Cat# P0704) for 1 hour at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... coli or Expi293™ cells underwent denaturation at 95 °C for 10 min in glycoprotein denaturing buffer (New England Biolabs) followed by immediate cooling on ice for 10 s ...
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bioRxiv - Biochemistry 2021Quote: ... 100 U PNGase F (New England Biolabs) was lyophilized (for up to 5 μg trimer) ...
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bioRxiv - Biochemistry 2021Quote: ... The optimal PNGase F (Cat. P0709S, NEB) concentration (relative to the glycoprotein substrate ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were treated with PNGase F (NEB) following the manufacturer’s instructions or mock treated as a control ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 µl PNGase F (NEB P0704S), 3µl 10% NP40 ...
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bioRxiv - Immunology 2020Quote: ... Enzymes PNGase F (P0704S, New England Biolabs) and Endo-Hf (P0703L ...
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bioRxiv - Biochemistry 2020Quote: ... PGNase F or EndoH (New England Biolabs) using the buffers and protocols provided by the supplier ...
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bioRxiv - Physiology 2019Quote: ... and 8 μl of PNGase F (NEB) were added ...
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bioRxiv - Cell Biology 2021Quote: ... or N-glycosilase F (PNGaseF, NEB P0704S), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... or PNGase F (P0704S, New England Biolabs) for 2 hours prior to electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: PNGase F digestions (New England Biolabs #P0704S) were performed according to the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2023Quote: ... 100 U PNGase F (New England Biolabs) was lyophilized ...
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bioRxiv - Cell Biology 2023Quote: ... and PNGase F (500U)(Cat# P7367, Biolabs) in 1 x GlycoBuffer-2 (Biolabs) ...
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bioRxiv - Developmental Biology 2021Quote: ... After annealing the complex an equimolar amount was mixed with 1000 ng Cas9 recombinant protein (NEB; final concentration 20 ng/μL) and incubated at RT for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Unligated 3’ linker was removed by incubating the samples with the 5’-deadenylase KmHnt3 (see Recombinant protein expression and purification) and RecJ exonuclease (New England Biolabs, M0264S) for 45 min at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: Recombinant Cas9 (New England Biolabs, USA) was used to generate a standard curve (20µM ...
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bioRxiv - Physiology 2022Quote: ... Recombinant CK2 was purchased from NEB. Recombinant TGFBR1 was purchased from Proqinase ...
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bioRxiv - Biophysics 2021Quote: ... 3 mM CaCl2 and 0.02% DDM) was incubated with 2000 units of N-glycosidase F (PNGase F) (New England BioLabs) at room temperature for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were resuspended in PBS and in some cases treated with N-glycosidase F (PNGase F; New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... 37°C with vaccinia virus capping enzyme (New England Biolabs), as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and Moloney murine leukemia virus reverse transcriptase (New England Biolabs). Using a QuantStudio 3 real-time quantitative PCR machine (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... 20µg of protein lysates and media samples were incubated with glycoprotein denaturation buffer at 95°C for 10 min and treated with 0.3µl Endo H (NEB; Catalog no #P0702) enzyme in glyco-buffer 3 for one hour at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were first denatured with Glycoprotein Denaturing Buffer at 65°C for 15 min and then treated with Endoglycosidase H (Endo H) (New England Biolabs, #P0702S) or Peptide-N-Glycosidase F (PNGase F ...
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bioRxiv - Biochemistry 2020Quote: 20 μg of protein from SK-RC-39 cells were treated with N-glycosidase F (PNGase F) and Endoglycosidase H (Endo H) (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: 30 to 50 μg proteins were digested for 90 min at 37°C with endoglycosidase-H (Endo-H; P0702L) or endoglycosidase-F (Endo-F; P0705S) as recommended by the manufacturer (New England Biolabs).
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bioRxiv - Biochemistry 2020Quote: ... 1 μl of PNGase F (non-reducing, NEB) was added to remove protein N-glycosylations and the sample was incubated at 50°C for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... and 8U PNGase F (P0704L, NEB; P7367, Sigma) were used ...
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bioRxiv - Microbiology 2021Quote: ... treated or not treated with PNGase F (BioLabs) for 2 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... One microliter of PNGase F (New England Biolabs) was added and the deglycosylation reaction proceeded during two hours at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... One microliter of PNGase F (New England Biolabs) was added and the deglycosylation reaction proceeded during two hours at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed using PNGase F (New England Biolabs) without a heat-inactivation step ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μL of PNGase F (NEB Cat # P0704S) was added to the reaction and the digestion allowed to proceed at 37° C for 1 hour ...