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Citations for New England Biolabs :
1 - 50 of 8705 citations for NMB SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and SAP (NEB, M0371S) treatment ...
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bioRxiv - Genomics 2020Quote: ... and SAP (NEB, M0371S) treatment ...
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bioRxiv - Systems Biology 2019Quote: ... and shrimp alkaline phosphatase (SAP, NEB). The concentrations of the caps ...
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bioRxiv - Genomics 2024Quote: ... followed by dephosphorylation with Quick SAP (NEB). gRNAs were re-folded prior ABE8e:gRNA complexation ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were treated with Shrimp Alkaline Phosphatase (SAP; from NEB). In short ...
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bioRxiv - Genomics 2019Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 µl at 37 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 μl at 37°C for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were processed according to the Exo-SAP protocol (NEB) and Sanger-sequenced by Azenta Life Services GeneWiz with hisC-ins-f as sequencing primer ...
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bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
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bioRxiv - Biochemistry 2020Quote: ... the pTXB1 plasmid was double-digested with Nde-I and Sap-I (New England Biolabs, Ipswich, MA, USA) enzymes at the respective restriction sites to generate binding sites for insert ligation ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified products were cleaned using Exonuclease-Shrimp Alkaline Phosphatase (Exo-SAP) mixture (New England Biolabs, Ipswich, Massachusetts) and sequenced bidirectionally using BigDye v3.1 terminator kit in ABI 3500XL Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were run by electrophoresis on a 2% agarose gel to ensure the absence of primer-dimers and PCR samples for each primer pair-opsin target were purified using Exonuclease (EXO) and Shrimp alkaline phosphatase (SAP) (NEB) prior to Sanger Sequencing to confirm gene-specific amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of DNA isolate was used for PCR (primers are in supplementary table 1.4) and PCR cleanup was performed using Exo-SAP (NEB, M0293, M0371). Sanger Sequencing reactions were prepared using (Thermo Fisher # 4337455) ...
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bioRxiv - Plant Biology 2021Quote: ... 500 ng of the SAP-treated RNA was treated with 12.5 units RNA 5’ Pyrophophohydrolase (RppH; New England BioLabs; Ipswitch, MA, USA) in 1X T4 RNA Ligase Buffer (New England BioLabs ...
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bioRxiv - Microbiology 2024Quote: PCR products of Asaia positive samples were cleaned up using Exonuclease I (Exo I) and Shrimp Alkaline Phosphate (Exo-SAP protocol) according to the protocol of New England Biolabs (NEB, MA, and USA) and sent for sequencing ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Neuroscience 2021Quote: ... T7 HiScribe kit (NEB) was used to transcribe the sgRNA DNA template with the appended T7 promoter ...
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bioRxiv - Genomics 2021Quote: ... using the Golden Gate assembly kit (NEB® Golden Gate Assembly Kit #E1601). Each guide sequence was cloned with its own U6 promoter and was followed by a sgRNA scaffold ...
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bioRxiv - Microbiology 2023Quote: ... A RNA cleanup kit (Monarch RNA Cleanup Kit T2050, New England Biolabs, France) was used to further clean the RNA samples ...
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bioRxiv - Synthetic Biology 2022Quote: ... plasmid (Monarch® PCR mini-prep kit) and gel extraction kits (Monarch® DNA gel extraction kit) were also obtained from NEB and used according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Developmental Biology 2021Quote: ... using HiScribe Sp6 kit (NEB) instead of T7.
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bioRxiv - Neuroscience 2021Quote: ... rRNA depletion kit (NEB #E6310), and Ampure XP beads (Beckman #A63881) ...
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bioRxiv - Microbiology 2020Quote: ... the PNGase kit from (NEB) was used per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... NEBNext rRNA Depletion kit (NEB) was used to remove bacterial and eukaryotic rRNA from total RNA isolated from pooled samples ...
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bioRxiv - Genetics 2022Quote: ... HiFi Assembly Cloning Kit (NEB) to insert the LYP1 homology sequence into the BamHI/EcoRV digest of Addgene plasmid #35121 to create the final backbone plasmid BFA0190 (Supplementary Figure 10) ...
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bioRxiv - Genomics 2022Quote: EnGen sgRNA synthesis kit (NEB)
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bioRxiv - Genomics 2022Quote: Monarch RNA Cleanup kit (NEB)
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bioRxiv - Microbiology 2022Quote: ... LunaScriptTMSuperMix Kit (New England BioLabs), SYBR qPCR Master Mix (ChamQTMUniversal ...
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bioRxiv - Microbiology 2022Quote: ... or NEBase Changer–Kit (NEB) and primers listed in supplementary Table 1 ...
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bioRxiv - Genetics 2022Quote: ... Gibson assembly cloning kit (NEB) was applied to ligate vector and GFP∷PAB-1∷3xFLAG fragment to generate plasmid pQZ2 ...
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bioRxiv - Microbiology 2021Quote: ... The A-Tailing Kit (NEB) was then used to add additional deoxyadenosine (A ...
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bioRxiv - Neuroscience 2022Quote: ... Quick Ligation Kit (M2200L, NEB) was used for the ligation of these appropriate clone ...
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bioRxiv - Biochemistry 2022Quote: ... using Q5 mutagenesis kit (NEB). N-(-42D ...
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bioRxiv - Cell Biology 2022Quote: ... using Gibson Assembly kit (NEB). To test the interaction of KRP4 and KRP5 ...
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bioRxiv - Microbiology 2022Quote: ... LunaScript RT SuperMix Kit (NEB) was used to generate cDNA using 1μg RNA ...
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bioRxiv - Plant Biology 2023Quote: ... blunting (Quick Blunting kit, NEB), and re-ligating using T4 DNA Ligase (NEB).
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bioRxiv - Genomics 2023Quote: ... or RNA cleanup kit (NEB) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Q5 DNA polymerase kit (NEB) was used to make amplicons ...
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bioRxiv - Biophysics 2023Quote: ... Gibson Assembly Kit (#E5510S, NEB) was used following the manufacturer’s instruction ...
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bioRxiv - Synthetic Biology 2023Quote: Monarch Plasmid MiniPrep kit (NEB) for isolating template plasmid and assembled plasmid from E ...
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bioRxiv - Biochemistry 2019Quote: ... we used a commercial assay kit (Ph.D.-7 Phage Display Peptide Library Kit, New England Biolabs) and followed the recommended protocol for “solution phase panning with affinity bead capture” with the following modifications ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA molecules were purified using a commercially available purification kit (MonarchQR Plasmid Miniprep Kit, NEB). The purified plasmids were run on a 0.8% agarose gel supplemented with 2.5 µg/mL of chloroquine in 1xTBE buffer containing 2.5 µg/mL of chloroquine at 25 V for 15 hours ...
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bioRxiv - Plant Biology 2020Quote: ... using the Gibson assembly kit (NEB). The pET28b vector was linearized by digesting with BamHI and XhoI to create overhangs compatible for Gibson assembly ...
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bioRxiv - Biophysics 2020Quote: Using PURExpress kits (New England Biolabs), 37.5 μL IVT reactions were assembled on ice with 1.5 μl RNase inhibitor ...