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Citations for New England Biolabs :
401 - 450 of 9410 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and NGS libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB). Illumina sequencing was performed with a NextSeq mid-output kit with 150-cycle single-end reads and automated adaptor trimming and demultiplexing (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... NGS libraries were pooled and quantified by qPCR using NEBNext Library Quant Kit (NEB). Sequencing was performed with NextSeq high-output kit with 75/150-cycle single-end reads and automated adaptor trimming and demultiplexing (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... ∼100 ng of RNA was treated with NEBNext rRNA Depletion Kit (New England Biolabs). RNA-Seq was performed using libraries prepared with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Illumina NGS libraries were prepared using the NEBNext Ultra II DNA Kit (NEB, E7645S), and sequenced for 50-nt SE reads ...
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bioRxiv - Genetics 2023Quote: ... and NGS libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB). Illumina sequencing was performed using the NextSeq platform with automated demultiplexing and adaptor trimming (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... RNA (>300 ng) was reverse transcribed using the Lunascript RT Supermix Kit (NEB, E3010).
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bioRxiv - Developmental Biology 2020Quote: High-throughput sequencing libraries from ChIP and input DNA were prepared using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370S). During library preparation ...
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bioRxiv - Developmental Biology 2021Quote: ... High-throughput sequencing libraries from ChIP and input DNA were prepared using NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370S). During library preparation ...
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bioRxiv - Systems Biology 2019Quote: ... The final pool was run on an Agilent Bioanalyzer dsDNA High Sensitivity chip and quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L). The quantified pool was hybridized at a final concentration of 400 pM and sequenced paired-end on the Illumina NovaSeq6000 platform using a S2 flowcell at 2×151 bp read lengths.
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bioRxiv - Molecular Biology 2019Quote: ... DNA obtained from the ChIP-assays was adaptor-ligated and amplified using the NEBNext multiplex oligo kit (New England BioLabs, E7335S) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The ChIP libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit for Illumina (Catalog: E7645S, New England Biolabs). The samples were subjected to various enzymatic steps to repair the ends and tailing with dA-tail followed by an adapter ligation ...
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bioRxiv - Molecular Biology 2023Quote: ChIP libraries were generated with input and pulldown DNA using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645L) for sequencing on an Illumina Nextseq with 75-bp read length and single-end.
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bioRxiv - Cell Biology 2024Quote: ChIP-seq libraries were prepared independently from two ChIP biological replicates using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E6177L) according to manufacturer’s instructions with a few modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... or the EM-seq Conversion Module (NEB, cat. No. E7125). To monitor the conversion rate ...
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bioRxiv - Genomics 2022Quote: ... The NEBNext® Enzymatic Methyl-seq Conversion Module (NEB #E7125) was used to perform a two-step enzymatic conversion of non-methylated cytosines to uracils ...
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bioRxiv - Developmental Biology 2023Quote: ... NEBNext Ultra II Directional RNA mRNA seq library (NEB; #E7760L) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PADIT-seq reporter libraries were first linearized with DrdI (NEB), which cuts a 12-bp DNA sequence (GACNNNN/NNGTC ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed from 1 µg of total RNA using Illumina’s TruSeq stranded mRNA Library Preparation Kit or from 500 ng total RNA using NEBNext Ultra II RNA -polyA+ (mRNA) Library Preparation Kit (New England Biolabs) following the manufacturer’s description.
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bioRxiv - Plant Biology 2023Quote: ... from 100 to 1000 ng of total RNA using a stranded kit (Illumina TruSeq Stranded Total RNA Kit or NEB Next Ultra™ II Directional RNA Library Prep Kit for Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting polyA-selected RNA was used to prepare RNA-seq libraries using the NEBNext® Ultra(tm) II Directional RNA Library Prep Kit for Illumina® (cat. #E7600, New England Biolabs), NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (cat ...
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bioRxiv - Plant Biology 2021Quote: ... 1.0 μg total RNA (0.1-0.5 μg for ovules) was used to generate mRNA-seq libraries using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, Cat# E7775). Sera-Mag Magnetic SpeedBeads (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RAD-seq library preparation was performed with the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts, USA) with the PstI restriction enzyme ...
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bioRxiv - Molecular Biology 2021Quote: ... was used to remove rRNA from Escherichia coli total RNA and RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA E7760L). Libraries were recovered with AMPure XP beads A63880 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770S, New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Input and CHART-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB) according to kit instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and final RNA-seq libraries generated using the NEBNext Ultra II Directional RNA Library Prep kit (NEB, E7760 and NEBNext Multiplex Oligos (NEB, E7335/E7500). Final libraries were sequenced as 150 bp paired-end reads on the Illumina HiSeq platform.
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bioRxiv - Bioengineering 2021Quote: ... The RNA-seq library was then generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Library quality was assessed using the Agilent Bioanalyzer 2100 system ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared using 1 µg of RNA and the NEBNext Ultra™ II RNA Library Prep Kit (NEB, E7775) with rRNA depletion following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... The library for RNA-Seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA). During the second cDNA synthesis dUTP was incorporated to maintain strand specificity ...
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bioRxiv - Immunology 2019Quote: Plate based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs Inc, E6420L). Cells from donor 364b were snap frozen in 10% DMSO in 90% BSA ...
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bioRxiv - Genomics 2021Quote: ... Strand-specific RNA-seq libraries were constructed using the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs). The libraries with adapters were sequenced on the Novaseq 6000 (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... strand-specific RNA-seq libraries were constructed by using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Two hundred nanograms of total RNA was subsequently processed to generate RNA-seq libraries using NEBNext Poly(A) mRNA Magnetic Isolation and Ultra II Directional RNA Library Prep kit for Illumina (NEB E7490, E7760) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Preparation of a small RNA-Seq library from submitted total RNA sample was performed using the NEBNext® small RNA library preparation kit (New England Biolabs, UK). Small-RNA sequencing was conducted using the Illumina HiSeq 4000 (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... strand-specific RNA-seq libraries were constructed by using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The library for RNA-seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA) and the sequencing was run on an Illumina HighSeq2000 with paired end reads ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA Magnetic Isolation Module and used to construct RNA-seq libraries with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to manufacturer’s protocol with slight modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were prepared from total RNA using the Ultra II RNA Sample Prep Kit (New England BioLabs, Ipswitch, MA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... RNA-seq libraries were prepared using the NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA). Sequencing was performed using HiSeq X Ten (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared from RNA samples (150ng) using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB Cat# E7405) in conjunction with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB Cat# E7765) ...
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bioRxiv - Molecular Biology 2024Quote: ... EM-seq libraries were prepared with the NEBNext® Enzymatic Methyl-seq kit (E7120S) according to the manufacturer’s protocol (New England Biolabs, MA USA). Briefly ...
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bioRxiv - Genomics 2024Quote: ... RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog #: E7760, New England Biolabs Inc.), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP samples and input were incubated with Proteinase K (NEB) at 65°C overnight to reverse formaldehyde crosslinking ...
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bioRxiv - Molecular Biology 2020Quote: ... The final pool was run on an Agilent Bioanalyzer dsDNA High Sensitivity chip and quantified using NEBNext Library Quant Kit for Illumina (cat # E7630L, New England Biolabs, Ipswich, USA).”
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries were generated using 10ng purified ChIP-DNA and the NEBNext®Ultra IIDNALibrary Prep Kit for Illumina® (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The final pool was run on an Agilent Bioanalyzer dsDNA High Sensitivity chip and quantified using NEBNext Library Quant Kit for Illumina (cat # E7630L, New England Biolabs, Ipswich, USA).
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bioRxiv - Cell Biology 2023Quote: ... ChIP and input libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswitch, MA, USA). Libraries were sequenced on an Illumina NextSeq500 (Illumina ...
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bioRxiv - Immunology 2021Quote: ... and 60 ng were used for the One Taq RT PCR Kit (New England Biolabs) according to the instructions provided by the manufacturer for mRNA quantification and reverse transcription (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: RNA (500 ng) was reverse transcribed using a LunaScript RT SuperMix kit (New England Biolabs) according to the manufacturer’s instructions in a total 20 µL reaction mix ...
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bioRxiv - Genomics 2023Quote: ... NGS sequencing libraries were generated using NEBnext Ultra II directional RNA library prep kit (NEB) following manufacture’s protocol ...