Labshake search
Citations for New England Biolabs :
551 - 600 of 9269 citations for Multiplex Assay kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Small RNA-seq libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, United States). Small RNAs were enriched using bead-based size selection ...
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bioRxiv - Developmental Biology 2022Quote: ... All samples had an RQN > 7.5 and strand-specific sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® in conjunction with the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs).
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bioRxiv - Plant Biology 2021Quote: ... Samples were PCR-amplified for 14 cycles and samples-specific dual indices (“NEBNext Multiplex Oligos for Illumina®”, E7600; New England BioLabs, Ipswich, USA) were attached to the fragments.
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bioRxiv - Genomics 2019Quote: ... The microRNA-Seq library was constructed using NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) following the instructions provided by the manufacturer (NEB, E7300S/L, USA). The library construction was started with 800ng total RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were prepared from 1 μg of total RNA using the NEB Next Multiplex Small RNA Library Prep Set for Illumina (NEB #E7300S and #E7580S) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All DNA fragments were indexed using NEBNext Multiplex Oligos for Illumina (Dual Index Primer sets 1 and 2, New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Genomics 2021Quote: ... Amplification was performed following bisulfite conversion using primers from the NEBNext Multiplex Oligos for Illumina module (cat#: E7535L, New England BioLabs, Ipswich, MA, USA) and the Kapa HiFi Uracil+ PCR system (cat# ...
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bioRxiv - Immunology 2021Quote: Small RNA libraries were generated from each RNA sample at TCAG (Toronto, Canada) using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, United States) and sequenced on an Illumina HiSeq 2500 rapid-run flow cell to generate 50 nucleotide (nt ...
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bioRxiv - Plant Biology 2022Quote: ... from leaves was isolated for small RNA library construction using NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... One microgram of total RNA per biological replicate was employed for the construction of 50 bp single-end (SE) reads using NEBNext® Multiplex Small RNA Library Prep Set for Illumina™ (New England Biolabs, Inc.). Lastly ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... The NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® with the NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) were used to generate DNA libraries for sequencing (New England Biolabs, USA) as per the manufacturers protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... All treated RNA samples were used for library preparation using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB catalog # E7300/7580) according to the manufacturer protocol ...
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bioRxiv - Genomics 2022Quote: Small RNA libraries were prepared for each sample using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: ... We ligated adapters in the cDNA library using adapters and unique dual indexes from the NEBNext Multiplex Oligos for Illumina (New England Biolabs, catalog no. E6440L). All procedures were performed following the manufacturer protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA library was PCR amplified using KAPA HiFi HotStart ReadyMix (FisherScientific, 50-196-5217) and NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (NEB, E7600S). The library was purified twice using 1x AMPure XP Bead (BeckmanCoulter ...
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bioRxiv - Cancer Biology 2023Quote: ... The library was PCR amplified using KAPA HiFi HotStart ReadyMix (FisherScientific, 50-196-5217) and NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (NEB, E7600S). The library was purified twice using 1x AMPure XP Bead (BeckmanCoulter ...
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bioRxiv - Genomics 2024Quote: ... and half the elution was used as a template in a second round of 8-10 cycle PCR to prepare Illumina libraries using NEBNext multiplex oligos using the same conditions described above (NEB E7335L and E7500L). The final PCR product was purified using AMPure XP beads (Beckman Coulter A63882 ...
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bioRxiv - Microbiology 2022Quote: ... while Gluc and Cluc expression levels were determined using Biolux Gaussia or Cypridina luciferase assay kits (New England BioLabs) and a microplate reader ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 pmol of TP_MAAAPQKCAAA* mRNA (see “Toeprinting assays” section) and components of the PURExpress ΔRibosomes Kit (New England Biolabs). The reaction was incubated at 37ºC for 20 min and then diluted in 50 mM Hepes KOH pH 7.5 ...
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bioRxiv - Genetics 2022Quote: ... The cDNA libraries were adapter-ligated using adapters and unique dual indexes in the NEBNext Multiplex Oligos for Illumina (New England Biolabs, catalog no. E6440, E6442) and amplified using 12 PCR cycles ...
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bioRxiv - Genomics 2021Quote: ... each cDNA library was ligated with a NEBNext adaptor and barcoded with an adaptor-specific index (NEBNext® Multiplex Oligos for Illumina, NEB, catalog number: E7335S). Twelve libraries were pooled in equal concentration and sequenced using Illumina HiSeq 3000 platform (single end ...
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bioRxiv - Biophysics 2021Quote: ... Indel frequencies at the SaCas9 target site were assessed via a T7E1 assay with the EnGen Mutation Detection Kit (NEB), using the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Final Illumina libraries were assessed for quality using an Agilent Bioanalyzer DNA High Sensitivity Assay and qPCR quantification was performed using NEBNext Library Quant kit for Illumina (New England Biolabs). Individual libraries were pooled equimolarly ...
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bioRxiv - Genetics 2019Quote: ... in accordance with the manufacturer’s recommendations and were validated using the Agilent High Sensitivity DNA assay on the Agilent Bioanalyzer 2100 system and quantified by NEBNext Library Quant Kit for Illumina (New England Biolabs). After clustering of the index-coded samples ...
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bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrations were measured by Qubit RNA HS assay kit and 750 ng of Dnase-treated RNA was used for rRNA depletion by NEB Next rRNA depletion kit (Human/Mouse/Rat) ...
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bioRxiv - Plant Biology 2021Quote: ... All other mutations of the constructs used for in vitro and in vivo assays were introduced using a Q5 site-directed mutagenesis kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Biochemistry 2023Quote: A luminescence based translation inhibition assay was performed using an in-vitro translation PURExpress® Δ Ribosome Kit from (NEB) The constituents of the kit were incubated with 25 ng pMSR DNA template ...
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bioRxiv - Genetics 2019Quote: T7 endonuclease assays (M0302L, NEB) were performed following the manufacturer instructions.
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bioRxiv - Developmental Biology 2020Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Genetics 2022Quote: ... using a T7E1 assay (NEB), and compared to the activity of Tet2 sgRNA that has been shown to modify the mouse genome efficiently95 ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Genomics 2022Quote: ... Barcoding and enrichment of libraries was carried out using NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs; New England Biolabs, Cat. No. E6440). Paired-end sequencing (2×151bp reads ...
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bioRxiv - Microbiology 2021Quote: ... 3 µl of extracted RNA was used in a TaqMan one-step qRT/PCR assay (Luna® Universal One-Step RT-qPCR Kit, NEB). TaqMan analysis was carried out with primer/probe combination described in Table 1 and analysis performed on the QuantStudio™ 6 Flex System (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2020Quote: In vitro trans-translation assays were performed using the PURExpress In Vitro Protein Synthesis and Δ Ribosome kits (New England Biolabs). For trans-translation assays ...
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bioRxiv - Zoology 2020Quote: The 16S rRNA and gltA amplicons from PCR assays were cleaned with the ExoSAP-IT kit (E1050, New England Biolabs, US) and Sanger sequenced ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/μL was used for subsequent reverse transcription assay with Luna® Universal One Step RT-qPCR kit (E3005, New England Biolabs) according manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from three pooled killing assay spots (as detailed in the previous section) utilizing the Monarch® Genomic DNA Purification Kit (NEB). Quantification of genomic DNA copies was targeted at the kdpAB gene and carried out with the Naica® Crystal Digital PCR System using Sapphire chips (Stilla Technologies).
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bioRxiv - Genomics 2023Quote: ... All libraries were dual-indexed using 12 cycles of PCR amplification using NEBNext® Multiplex Oligos for Illumina® Dual Index Primer Set (New England Biolabs, Inc., Cat. No. E7600). Library quality control was performed by measuring concentration with Qubit® dsDNA HS Assay Kit (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2020Quote: ... a dsDNA HS qubit assay and also by a NEBNext library quantitation Assay (New England Biolabs) before sequencing on Illumina’s HiSeq2500v4
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bioRxiv - Developmental Biology 2021Quote: ... The T7E1 assay (Cat#M0302, NEB) was then applied as in (Sato et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutants selected for cloning were also analysed via T7 endonuclease I-based (T7E1) heteroduplex assay according to the manufacturers protocol (EnGen® Mutation Detection Kit, NEB #E3321), and the length of digested and undigested fragments were visually compared by gel electrophoresis (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... as well as wildtype (WT) N2aC were assayed for mis-matched DNA pairs via T7 endonuclease assay (EnGen Mutation Detection Kit; New England Biolabs, Ipswich, MA) with PCR primers targeted towards our genomic region of interest (Integrated DNA technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... 200 fmol of DNA (determined following quantification with the Qubit™ dsDNA HS Assay Kit) was processed using the NEBNext Companion Module for ONT Ligation Sequencing (NEB, #E7180). Sequencing adapters were added using the Ligation Sequencing Kit (ONT ...
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bioRxiv - Cancer Biology 2022Quote: ... We designed spCas9 guide RNAs to target codon 12 of the human KRAS gene and selected the one with highest targeting efficiency determined by T7E1 mismatch assay (EnGen Mutation Detection Kit, New England Biolabs, Ipswich, MA). Truncated guide RNA was produced by PCR assembly of a guide RNA template (56 ...
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bioRxiv - Biochemistry 2023Quote: ... MBP pulldown assays were performed similar to GST pull down assays using Amylose resin (New England Biolabs) and 1 nmol of MBP-Oskar 292-325 and 5 nmol of Smaug 37-281.
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Genetics 2022Quote: ... T7 Endonuclease I assay (T7EI; NEB M0302L) or restriction enzyme digestion using Mse I (NEB R0525L) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the T7E1 assay (NEB #E3321) to determine potential mutations for every single fly following the protocol in (Zhu et al. ...
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bioRxiv - Cell Biology 2023Quote: ... a T7 endonuclease I cutting assay (NEB) was used to identify the extent of insertions/deletions for each guide ...