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Citations for New England Biolabs :
251 - 300 of 10000+ citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
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bioRxiv - Plant Biology 2020Quote: ... The NRPD1-Maltose Binding Protein (MBP) fusion protein was affinity purified using Amylose resin (New England Biolabs) and eluted with 20 mM maltose ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 µg of purified recombinant WRN protein was treated with Lambda Protein Phosphatase (LPP, New England Biolabs) in 1X PMP buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... the concentrated protein at 3 mg/mL was incubated with c-AMP Protein Kinase A PKA (NEB) (molar ratio of 40:1 ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted from 1 ml of overnight culture with the kit Monarch HMW DNA Extraction kit (T#3060 New England Biolabs; Ipswich, MA, USA) following the manufacturer instructions for High Molecular Weight DNA extraction from gram-negative bacteria ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-MBP (NEB, E8032). Peroxidase and Alexa-fluor conjugated secondary antibodies were purchased from Jackson Laboratories and ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-myc (New England Biolabs), rabbit polyclonal anti- GFP (gift from Oliver Gruss ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixed RNA was depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) and fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. no. E6150S) for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μg of purified protein complexes were treated with 2,000 U of Lambda Protein Phosphatase (New England Biolabs) in 200 μl dephosphorylation buffer (50 mM HEPES-NaOH ...
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bioRxiv - Systems Biology 2023Quote: ... the wild-type protein extracts were incubated with 40 units of Lambda Protein Phosphatase (New England Biolabs, P0753S) at 30°C for 30min ...
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bioRxiv - Biochemistry 2022Quote: ... purified Trx-FER-KD and FER-KDK565R proteins were treated with 1 μl of λ-phosphatase (λ-PP) (400,000 units/ml, New England Biolabs. P0753S) and 1 mM MnCl2 for 1-2 h at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Zebrafish embryos were injected at the 1-cell stage with a 1 nL solution of bbs2 gRNA (200 ng/μL) and Cas9 protein (10 μM; New England BioLabs, Beverly, MA). Mutagenesis was confirmed by High Resolution Melt Analysis (HRMA ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Genomics 2019Quote: ... expressing ATG7 isoform 2 was a gift from Toren Finkel (Addgene plasmid #24921).31 The plasmid pCMV-myc-Atg7(1) expressing ATG7 isoform 1 was derived from pCMV-myc-Atg7(2) using the Q5 site-directed mutagenesis kit (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... in protein kinase buffer (New England Biolabs) in the presence of 0.2 mM Mg2+-ATP and 1 µCi [γ32-P]ATP for 30 min at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... in protein kinase buffer (New England Biolabs) and 0.5 mM Mg2+-ATP for 30 min at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein Phosphatase Inhibitor 2 (New England Biolabs) and Trichostatin A HDAC inhibitor (TSA ...
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bioRxiv - Physiology 2022Quote: ... The Cas9 protein was purchased from NEB. Mixture of sgRNA (100 pg ...
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bioRxiv - Immunology 2022Quote: ... 1μl of Lambda Protein Phosphatase (NEB, P0753S) was added and samples were incubated at 30°C for 30 minutes ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to nitrocellulose membranes (Biolabs). Membranes were then incubated with primary antibody diluted in 2.5% milk or 2.5% BSA in Triton X-100-TBS buffer (T-TBS ...
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bioRxiv - Cell Biology 2020Quote: ... protein tyrosine phosphatase (PTP, 5 unit; NEB), or sodium orthovanadate (Na3VO4 ...
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bioRxiv - Plant Biology 2021Quote: ... Lambda protein phosphatase treatment (New England BioLabs) was performed following the manufactorer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Lambda protein phosphatase (New England BioLabs, P0753S) was used per product instructions.
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bioRxiv - Pathology 2021Quote: Magnetic protein G beads (New England Biolabs) were blocked in antibody selection buffer and used to immobilize 10-15μg polyclonal rabbit anti-VWF (Dako A0082 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were degraded using proteinase K (NEB) and RNA was purified using RNeasy maxi kit (Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... Prestained protein ladders (P7712 or P7719, NEB) on the membranes were annotated by WesternSure Pen (LI-COR).
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bioRxiv - Developmental Biology 2022Quote: ... EnGen Spy Cas9 NLS protein (NEB, M0646) was used for F0 experiments.
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bioRxiv - Microbiology 2023Quote: ... coli Protein Synthesis System (New England Biolabs), then purified with Ni-NTA beads (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... gRNAs and Cas9 protein (NEB, cat# M0251S) were simultaneously injected into the embryos at one-cell stage.
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bioRxiv - Microbiology 2024Quote: ... coli protein synthesis system (New England Biolabs) was used for in vitro protein expression ...
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bioRxiv - Cell Biology 2024Quote: ... Protein A magnetic beads (New England Biolabs) were saturated with rabbit-anti-GFP ...
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bioRxiv - Developmental Biology 2022Quote: ... (2019): 1) the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) was used for library preparation ...
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bioRxiv - Genomics 2019Quote: ... mRNAs were isolated from 1 μg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared using the NEBnext Ultra RNA library prep kit for Illumina (NEB) ...
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bioRxiv - Genetics 2019Quote: ... mRNAs were isolated from 1 μg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared following instructions from NEBs Ultra Library Preparation Kit for Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg total RNA was depleted of rRNA using the NEBNext rRNA Depletion kit (NEB). To capture nascent RNA ...
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bioRxiv - Cell Biology 2022Quote: ... and the amplicons were extracted from a 1% agarose gel (Monarch Gel Extraction Kit, NEB). Inserts were then cloned into a pCDH1 lentiviral vector ...
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bioRxiv - Cell Biology 2021Quote: Whole cell lysates or eluted proteins after affinity purification were treated with Protein Deglycosylation Mix II (NEB, Cat# P6044), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Deglycosylation of purified protein was performed in non-denaturing conditions according to manufacturer’s protocol (Protein Deglycosylation Mix II, NEB).
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bioRxiv - Microbiology 2022Quote: ... We then used 1:3 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). After the HiFi assembly ...
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bioRxiv - Immunology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). The Hifi assembly products were Ampure bead purified and eluted into 20 µL of H2O ...
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Biochemistry 2020Quote: ... was incubated for 30 minutes shaking at 1.500 rpm at room temperature with protein extract (500 µg of whole cell protein extract or 150 µg of CPE) and murine RNase Inhibitor (NEB) in the corresponding protein extraction buffer (without glycerol) ...
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bioRxiv - Biophysics 2019Quote: ... uncleaved fractions and 3C protease were removed by Ni-chelated sepharose and the G protein was dephosphorylated by lambda protein phosphatase (NEB), calf intestinal phosphatase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: 750000 cells were harvested and resuspended in 40 μL lambda protein phosphatase reaction buffer (1X NEBuffer Pack for Protein MetalloPhosphatases (NEB), 1mM MnCl2 (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... was expressed as maltose-binding protein-tagged fusion protein using the pMAL(tm)c5X-vector (New England Biolabs, Ipswich, USA). The coding DNA sequence was amplified by PCR using gene-specific primers (for primer sequences ...
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bioRxiv - Plant Biology 2021Quote: The MBP-NbERF-IX-33a fusion protein was prepared using the pMAL Protein Fusion and Purification System (New England Biolabs). E ...
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bioRxiv - Plant Biology 2020Quote: ... and an aliquot containing 10 µg protein was treated with lambda protein phosphatase reaction mix following the instructions of the manufacturer (New England Biolabs) for 1 h at 30°C.
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bioRxiv - Cell Biology 2019Quote: ... were expressed as fusion proteins with an N-terminal maltose binding protein (MBP)-tag using the pMALTMc5x-vector (New England Biolabs). Cloning was mediated by the addition of the restriction sites XmnI/PstI to the ends of gene fragments PCR-amplified from P ...
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bioRxiv - Plant Biology 2023Quote: ... The MBP-OsTLP protein or the MBP control protein was bound to amylose resin (New England Biolabs, Beverley, MA, USA), and then the LssaCA-His protein was added to the beads ...
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bioRxiv - Plant Biology 2024Quote: ... An aliquot containing 10 mg of protein was then subjected to lambda protein phosphatase reaction mix following the manufacturer’s instructions (New England Biolabs, US) for 1 hour at 30°C ...