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Citations for New England Biolabs :
601 - 650 of 10000+ citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2019Quote: ... protocol using the PCR Barcoding Expansion 1-12 (EXP-PBC001) kit (ONT) and LongAmp Taq 2× Master Mix (New England Biolabs, MA) with the following thermocycling conditions ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Synthetic Biology 2021Quote: Expression units were assembled from cloned level 0 parts into level 1 destination vectors provided by the MoClo kit using 40fmol of each vector and 20 U of BsaI-HFv2 (NEB, R3733L) with remaining conditions as described above ...
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bioRxiv - Genetics 2019Quote: ... Poly(A)+ RNA was selected from 1 μg of total RNA using the NEBNext Ultra Kit (New England Biolabs, Ipswitch, MA). Libraries were prepared and sequenced by Genewiz (South Plainfield ...
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bioRxiv - Genomics 2019Quote: To prepare the RNA sample for use in a smallRNA library preparation kit the sample was phosphorylated using 5 ul of 10X T4 PNK buffer and 1 ul of T4 PNK (NEB #M0201), 1 ul of SUPERASE-In ...
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bioRxiv - Immunology 2022Quote: ... The library for sequencing was prepared using 1 µg of RNA and the NEBNext Ultra kit (New England BioLabs, Ipswich, MA), according to the protocol for low-input samples ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA was quantified by ultraviolet spectrophotometry and 1 μg used for reverse transcription by LunaScript® RT SuperMix Kit (New England Biolabs), as described by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
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bioRxiv - Genomics 2023Quote: ... the samples were incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Cancer Biology 2023Quote: ... with 1 µg of total RNA used for sequencing library construction with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). Raw FastQ files were mapped using Bbsplit from the Bbtools 39.01 suite against Mm10 and hg38 (Bushnell ...
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bioRxiv - Bioengineering 2023Quote: ... and a gBlock (purchased from Integrated DNA Technologies [IDT]) containing an anhydrotetracycline (aTc) inducible promoter (pLTetO-1) were inserted via HiFi Assembly (HiFi Assembly Kit (NEB#E5520S)) into a PCR-ed fragment of plasmid pBbA2c-RFP (Addgene #35326 ...
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bioRxiv - Developmental Biology 2023Quote: ... pCS2-dKif6 (1-501)-EGFP was generated from the pCS2-dKif6-EGFP plasmid by Gibson assembly (NEB HiFi DNA Assembly Kit) using a GeneArt gene fragment encoding amino acids 1-501 (Thermo Fisher).
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bioRxiv - Developmental Biology 2023Quote: ... was combined with either MEP-1 or Mi-2 PCR-amplified coding sequences in a Gibson assembly reaction using NEBuilder® HiFi DNA Assembly kit (NEB) following manufacturers protocols ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... The annealed inserts were digested with EcoRI/AgeI for PLKO.1 and BsmBI for LentiCRISPRv2 and ligated using the Quick Ligation Kit (NEB M2200S).
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bioRxiv - Genetics 2023Quote: ... Index primers set 1 and 2 from the NEBNext Multiplex Oligos for Illumina kit (New England Biolabs, E7335S/L E7500S/L) were incorporated using Herculase II Fusion Polymerase Kit (Agilent ...
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bioRxiv - Pathology 2023Quote: Sequencing library was generated from total mRNA (1 µg) using NEBNext UltraTM RNA Library Prep Kits for Illumina (New England BioLabs, MA). cDNA libraries were sequenced by a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2023Quote: ... 1 µg of genomic DNA was pooled with 1:20 dilutions of unmethylated lambda (1 µl of 0.1 ng/µl) and methylated pUC19 control DNA (1 ul of 0.005 ng/µl) from the EM-seq kit (E7120L; NEB, Ipswich, MA). Volumes were made up to 50 ul with 0.1x TE buffer (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the tissue samples were stained using Vizgen’s Cell Boundary Kit (Vizgen, 10400009) and blocked in blocking solution (Vizgen, 20300012) supplemented with a 1:20 dilution of Rnase inhibitor (NEB, M0314L) for one hour ...
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bioRxiv - Cancer Biology 2023Quote: ... These products were then purified by separation on a 1.0% agarose gel at 100 volts constant for 1 hour and were then purified via the Monarch DNA gel extraction kit (NEB, #T1020L). Purified products were quantified with qubit high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: Immunoglobulin libraries were generated from 1 μg of RNA using the NEBNext Immune Sequencing Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... was synthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). The sequences of the primers used for RT-PCR are listed in Table S2.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were barcoded using Illumina i5 and i7 adaptors from NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) kit (New England Biolabs) and sequenced using a 150-cycle NextSeq 500/550 High Output Kit v2.5 with 75 forward and 75 reverse reads ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared with 0.1-1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalogue #E6560L) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genomics 2024Quote: ... The digest product was run on a 1% agarose gel and the band was excised and purified using a Monarch DNA Gel Extraction Kit (NEB #T1020) following the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... was synthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). For RNA-sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 13000 x g for 1 minute and the supernatant was once more purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs). We expect the “crush and soak” method to improve signal strength if low labeling densities are used during extension.
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bioRxiv - Microbiology 2024Quote: ... A fragment containing the CRISPR array plus the extra BsrG1 restriction site was cut from the synthesized pUC19 by digestion with DraI and BstZ17I enzymes and cloned into the BsaI-linearized pSTU-1 plasmid using the NEBuilder HiFi cloning kit (New England Biolabs, UK). The plasmid sequence was confirmed by sequencing (Eurofins Genomics ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was quantified by Nanodrop and 1 µg of RNA per sample was treated for ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (NEB, E6350) and then immediately followed by Library prep using NEBNext Ultra II kit (NEB ...
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bioRxiv - Plant Biology 2019Quote: ... MBP-tagged proteins were purified using amylose resin (New England Biolabs, #E8021S) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... and proteins were then deglycosylated with PNGaseF (New England Biolabs, Ipswich, MA) for 2 hours at 37C ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Protein-bound RNAs were end-repaired on beads using T4 PNK (NEB) and 3’-end-labeled with azide-dUTP using yeast poly(A ...
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bioRxiv - Cell Biology 2020Quote: ... MBP-TRIM17 proteins were purified on amylose resin (New England BioLabs, #E8021L) and then eluted in a buffer containing 20 mM maltose before dialysis in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and 800 units of lambda protein phosphatase (λ-PPase; New England Biolabs) and incubated for 20 min at 30°C under slight agitation ...
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bioRxiv - Cell Biology 2020Quote: ... Interacting MBP-AtPIP5K2 protein was detected using an anti-MBP antibody (NEB). Protein input was detected using an anti-GST antibody (GE Healthcare).
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: For analysis of protein N-glycosylation PNGase F (P0704S, New England Biolabs) was used and the assay was performed according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein purification was carried out using an amylose resin purification system (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... 0.75 M NaCl) and 2 μl protein kinase K (NEB, Ipswich, USA) during 2 h at 60 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... S protein were first incubated with either 1000U/mL PNGase F (NEB) or deactivated PNGase F at 37 °C for 14 h ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µl of a broad range protein marker (New England Biolabs, #P7708) was run alongside the samples on each gel to estimate the molecular weight ...
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bioRxiv - Biochemistry 2019Quote: ... unlabeled AdoMet was left out and 6 μg of MBP2* protein (NEB) was added to each reaction to increase molecular crowding.
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bioRxiv - Biochemistry 2019Quote: ... The protein was then passed over a 2 mL amylose column (NEB) and washed with 3 CV of Flag Wash Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Each Prx protein variant was generated through Q5 mutagenesis (New England Biolabs). The described primers were ordered from Integrated DNA technologies (IDT ...
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bioRxiv - Biochemistry 2020Quote: ... 30 nM dCas9 protein (dCas9 Nuclease, S. pyogenes, M0652, New England Biolabs), 1–4× 104 Beads@oligo ...
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bioRxiv - Biophysics 2021Quote: RecA protein from Escherichia coli was purchased from New England BioLabs (NEB), Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were dephosphorylated using 1μL Lambda phosphatase at 30 °C (NEB; P0753S) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... MBP-tagged proteins were purified by amylose affinity chromatography (New England Biolabs) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... a catalytic subunit of protein kinase A (PKA Cα, New England Biolabs); Ca2+/calmodulin-dependent protein kinase II (CaMKII ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... the recombinant proteins were purified by amylose resin (#E8022S, New England Biolabs) according the manual instructions ...