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Citations for New England Biolabs :
551 - 600 of 2294 citations for Mouse PNP shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid constructs were modified by site-directed mutagenesis (E0554; New England Biolabs) to create the desired mutations in klp-6 and cwp-4 ...
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bioRxiv - Molecular Biology 2021Quote: PHO4 was cloned into the 2micron plasmid pRS424 using Gibson assembly (NEB). pGAL-H3-3xHA-URA3 on a single copy plasmid is a gift from the Michel Strubin lab.
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bioRxiv - Molecular Biology 2020Quote: ... After elimination of original plasmid template by digestion with DpnI (NEB R0176), amplification products were transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... The plasmid was simultaneously digested with restriction endonucleases BsaI and BstXI (NEB). The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit) ...
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bioRxiv - Cell Biology 2022Quote: ... the LentiGuide-Hygro plasmid was digested using BsmBI-v2 enzyme (NEB #R0739), and the larger band (~9 kb ...
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bioRxiv - Molecular Biology 2021Quote: ... The resultant plasmids were digested with PacI and PmeI (New England Biolabs) and used to transfect L ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L). NEB 5-alpha Competent E ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into the control plasmid using restriction enzyme XhoI (NEB, #R146L). pCAGGS-Cre plasmid was generated as described (Kranz et al. ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid prib(+)XpA was digested by NruI and SnaBI (New England Biolabs) and ligated to produce prib(+)XpA lacking the poliovirus capsid-encoding region from 1175 to 2956 (prib(+)XpAΔ-1175-2956).
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bioRxiv - Molecular Biology 2019Quote: 50 µg of the plasmid DNA were cut with HindIII-HF (NEB) in Cut Smart buffer with 100 U of enzyme for 1.5 hours at 37°C in 50 µl ...
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bioRxiv - Genomics 2019Quote: ... The 3XFlag-pA-Tn5-Fl plasmid was transformed into C3013 cells (NEB) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... dP75 plasmid with mutations were generated by Site-Directed Mutagenesis Kit (NEB). For yeast two-hybrid assays ...
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bioRxiv - Microbiology 2021Quote: ... The parental plasmid was digested for 4 h using Dpn1 endonuclease (NEB) at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... expression plasmids pETgshA and pETgshB were assembled using HiFi Assembly MasterMix (NEB), and transformed into chemically competent E ...
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bioRxiv - Genomics 2021Quote: ... and cloned into plasmid pT2 using Gibson Assembly Cloning Kit (NEB, E5510S). Obtained pT2_FF plasmids were used as templates for PCR-based barcoded library preparation (22).
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bioRxiv - Cell Biology 2021Quote: ... This first intermediate plasmid was linearized via EcoRV (New England Biolabs-NEB) digestion and 2 inserts were added to it by In-Fusion to generate the second intermediate plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... This first intermediate plasmid was linearized via EcoRV (New England Biolabs-NEB) digestion and 2 inserts were added to it by In-Fusion to generate the second intermediate plasmid ...
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bioRxiv - Microbiology 2020Quote: The plasmid pAAV S1-Fc was digested with New England Biolabs (NEB) Restriction Enzymes Pvu I-HF (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Cas9 and dCas9 plasmids were linearized with the PacI restriction enzyme (NEB) to enable plasmid integration at the NEUT5L locus ...
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bioRxiv - Microbiology 2022Quote: ... The linear plasmid pCAG-MYOC cut by the BstEII restriction enzyme (NEB) was purified ...
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bioRxiv - Molecular Biology 2022Quote: About 3 μg plasmid was digested with 20 units SapI (NEB, R0569) and dephosphorylated with 3 units rSAP (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... All fragments for plasmids cloning were amplified using Q5 DNA Polymerase (NEB) and the plasmids were assembled by isothermal assembly ...
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bioRxiv - Microbiology 2022Quote: ... plasmids were linearized by incubation overnight with MfeI (McMillan; New England Biolabs) or NotI (IMP181 ...
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bioRxiv - Plant Biology 2022Quote: ... The plasmid was digested with XhoI and XbaI (New England Biolabs, UK). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... Laconic sensor mRNA was synthesised from pCS2 plasmids linearised with NotI (NEB), with mMESSAGE mMACHINE SP6 Transcription Kit (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... and ligated into the preferred plasmid using Quick ligase (New England BioLabs). The plasmids used in this study are listed in Table 1 ...
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bioRxiv - Plant Biology 2021Quote: ... The homology plasmids were assembled using HI-Fi DNA Assembly Mastermix (NEB) with four PCR-generated DNA fragments (see Table S2 for primers) ...
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bioRxiv - Molecular Biology 2019Quote: ... The plasmid was linearized by restriction digest with PmeI and SbfI (NEB) and incorporated into the genomes of CM018 and Kn99a by biolistic transformation (Chun and Madhani 2010) ...
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bioRxiv - Synthetic Biology 2020Quote: ... before being ligated to plasmid vectors using T4 DNA Ligase (NEB, M0202). NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: Mutated plasmids were digested with BamHI restriction enzyme (NEB, Ipswich, MA, USA), purified and used for in vitro transcription reaction as described above ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting plasmid was digested with NotI and KasI (New England Biolabs) to remove a truncated kanR gene upstream of arif-1 ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmids expressing sgRNAs were cloned using T4 DNA Ligase (New England Biolabs).
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bioRxiv - Cell Biology 2021Quote: ... through ligation of both plasmids after digestion with BamHI (NEB, Cat# R313614) and BspEI (NEB ...
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bioRxiv - Synthetic Biology 2019Quote: ... Prepared plasmid DNA was linearized using SmaI (New England Biolabs, Ipswich, MA) digestion for 4 hours at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... Each probe was amplified from the corresponding plasmid using either OneTaq (NEB) or Taq polymerase (Roche) ...
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bioRxiv - Synthetic Biology 2021Quote: ... digested pU6 plasmid backbone using 1μl of T4 PNK (NEB cat #M0201L) and 1μl of T4 ligase (NEB cat #M0202L ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Separately seeded cells were transfected with pCMV-GLuc2 plasmids (1 μg, NEB) and GLuc activity was measured using the same kit ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid was transformed into Escherichia coli expression strain BL2lλDE3 (New England Biolabs) for expression ...
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bioRxiv - Genetics 2019Quote: ... The pX459 plasmid was digested with High Fidelity BbsI-HF (NEB #R3539) at 37°C for 10 min followed by gel purification using NucleoSpin Gel and PCR Clean-up Midi kit (#740986.20 ...
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bioRxiv - Developmental Biology 2019Quote: ... the plasmid was linearized using either XbaI and Kpn1 restriction enzymes (NEB), and SP6 and T7 polymerases (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... after digestion of the template plasmid with 20 U Dpn I (NEB) for 1 h at 37°C ...
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bioRxiv - Genetics 2020Quote: Circular plasmids was removed by digestion with DpnI (NEB; cat. no. R0176) at 37°C for 30min ...
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bioRxiv - Biochemistry 2021Quote: ... 16.5 µg pNIC28-Bsa4 plasmid was digested with 60 U BsaI (NEB) in 100 µL following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... 7 μg pNZdmsC3GH plasmid was digested with 40 U of SfiI (NEB), separated on a 1% agarose gel and ...
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bioRxiv - Biochemistry 2021Quote: Plasmid templates for all EM analyses were linearized with Msc I (NEB).
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bioRxiv - Physiology 2021Quote: ... PCR fragments and plasmid backbones were assembled using Gibson assembly (NEB – E2611). For Gateway cloning ...
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bioRxiv - Biophysics 2021Quote: ... The cosmid-I95 plasmid was amplified in Neb5 (New England Biolabs, C2987H) and the DNA was purified using a Qiafilter plasmid midi kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Linear transformation cassettes were excised from plasmids with EcoRV (New England Biolabs) prior to transformation.
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bioRxiv - Genetics 2021Quote: ... All plasmids were assembled with NEBuilder HiFi DNA Assembly Cloning Kit (NEB). See Figure 1A.