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Citations for New England Biolabs :
601 - 650 of 2293 citations for Mouse ONECUT3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Plasmids were methylated using SssI (New England Biolabs, Frankfurt am Main, Germany) as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... The inserts were ligated into the plasmid using the Quick Ligase (NEB) and the reaction was used to transform DH5α’s ...
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bioRxiv - Genetics 2020Quote: ... Purified plasmid DNA was digested with DraI (New England BioLabs, Beverly, MA) and used for in vitro transcription reaction to generate gRNA ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid prib(+)XpA was digested by NruI and SnaBI (New England Biolabs) and ligated to produce prib(+)XpA lacking the poliovirus capsid-encoding region from 1175 to 2956 (prib(+)XpA-Δ1175-2956).
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... plasmids were transformed into SHuffle T7 Express LysY bacteria (NEB Cat# C3030J) and induced with 0.4 mM of IPTG at 16°C for 24h ...
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bioRxiv - Cancer Biology 2022Quote: ... the target CAR plasmid backbone was linearized with BamHI-HF (NEB, R3136T) and cleaned up using Zymo Research DNA purification kit (Zymo Research ...
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bioRxiv - Biochemistry 2022Quote: ... Standard plasmid construction using restriction enzymes BspHI and XhoI (New England Biolabs) was used for UMAG_01820 ...
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bioRxiv - Biophysics 2022Quote: ... and Pat1 were cloned into a modified pMAL plasmid (New England Biolabs) using standard methods and NdeI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2022Quote: ... All PTet plasmids were generated using Gibson reactions (NEB HiFi Assembly Mix) from PCR products ...
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bioRxiv - Cell Biology 2022Quote: ... plasmid pMO014 was linearized with BglII (Cat. No. R0144, New England BioLabs) and transformed for integration into the genomic trp1 locus using a LiOAc-based protocol(14) ...
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bioRxiv - Genomics 2022Quote: ... Purified plasmids were electroporated to ElectroMAX Stbl4 Competent Cells (New England Biolabs) and amplified.
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bioRxiv - Plant Biology 2022Quote: ... plasmids were linearized with the NheI restriction enzyme (NEB, Ipswich, MA, USA) to transcribe complimentary RNA (cRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Mutations were introduced in plasmids with Q5® Site-directed mutagenesis (NEB). All the constructs were validated by DNA sequencing (Eurofins Genomics) ...
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bioRxiv - Microbiology 2022Quote: ... plasmid DNA was digested with restriction enzyme KpnI following manufacturer’s instructions (NEB). After two hours at 37°C the ultrapure DNA underwent phenol chloroform extraction and ethanol precipitation at -80°C as described previously for ultrapure DNA purification ...
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bioRxiv - Synthetic Biology 2022Quote: ... All enzymes for plasmid construction were obtained from New England Biolabs (NEB). Constructs were chemically transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... All other plasmids were constructed using a HiFi DNA assembly kit (NEB). All plasmids were confirmed by Sanger sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary to plasmid transformation of competent bacteria (New England Biolabs, Ipswich, USA), selection of clones ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified with the Monarch Plasmid Miniprep Kit (T1010, New England Biolabs). Plasmids were digested with restriction enzymes to generate linearized templates for in vitro transcription ...
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bioRxiv - Microbiology 2022Quote: ... A VSV-G plasmid (16) was digested with KpnI and HpaI (NEB), removing a C-terminal part of G ...
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bioRxiv - Molecular Biology 2022Quote: ... by combining the original plasmid digested with HindIII (New England Biolabs, #R3104S) and NotI (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: 100-ng pTXB1 plasmids were firstly linearized by 10U XbaI (NEB, R0145S) at 37°C for 1h and purified by Zymo columns ...
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bioRxiv - Microbiology 2022Quote: ... This plasmid was digested with high fidelity (HF) PstI and XhoI (NEB) before insertion of the amplified GPR1 using NEBuilder® HiFI DNA assembly Cloning kit ...
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bioRxiv - Microbiology 2022Quote: ... Therefore the CIp10 plasmid was digested with HF PstI and XhoI (NEB) before insertion of the amplified Gpr1 and the mTurquoise2 ...
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bioRxiv - Genetics 2023Quote: ... Linear pUC-GW plasmids and purified SEED cassettes were assembled by NEB assembly following commercial protocols for 1h at 50°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB), followed by transformation into NEB 5-alpha competent E ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were constructed by NEBuilder HiFi DNA assembly (New England Biolabs) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... The obtained yeast plasmid DNA was transformed into competent cells (NEB, C2987H). Yeast strains ...
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bioRxiv - Genetics 2023Quote: ... The BAC was purified purified using the Monarch Plasmid Miniprep Kit (NEB).
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bioRxiv - Synthetic Biology 2023Quote: ... integration plasmids were linearized in a BsaI restriction digestion (BsaI-HFv2, NEB) at 37 °C for 2 h ...
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bioRxiv - Systems Biology 2023Quote: ... the plasmid backbone was digested and dephosphorylated using BsmBI restriction enzyme (NEB) and Quick CIP (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... The ligated plasmid was transformed into Stable competent cells (New England Biolabs), and individual clones were picked and sequenced (Laragen ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of template plasmid was digested using 1µL AgeI (NEB, R3552S) for 15h in Cutsmart Buffer (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were assembled via Gibson Assembly (New England BioLabs 2X Master mix) (62 ...
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bioRxiv - Molecular Biology 2023Quote: ... Backbone plasmids were linearised via PCR followed by treatment with DpnI (NEB) before assembly with fragments using NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Genetics 2023Quote: Plasmid px458 was digested with restriction endonuclease BbsI (New England Biolabs, R0539S), by addition of 1μl enzyme to 1μg plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids verified by Sanger sequencing were linearized with AseI (NEB, Catalog # R0526S) and SspI (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... of the SARS-CoV-2 Positive Control (N gene) plasmid (NEB N2117). Sanger sequencing confirmed successful mutagenesis ...
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bioRxiv - Developmental Biology 2023Quote: ... from template DNA plasmid digested by NotI or SpeI (New England Biolabs), and were cleaned by RNeasy mini kit (QIAGEN) ...
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bioRxiv - Cancer Biology 2022Quote: ... Ligated plasmids were transformed into NEB stable cells (New England Biolabs #C3040) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The pET755 plasmid was used for Q5 polymerase-mediated (New England Biolabs) PCR amplification of the backbones that contain an ampicillin resistance cassette and unc-54 3’utr ...
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bioRxiv - Immunology 2023Quote: ... The LZRS-CAR-IRES-eGFP plasmids were overnight digested with BamHI (NEB) and purified using Zymoclean Large Fragment DNA Recovery Kit (Zymo ...
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bioRxiv - Genetics 2023Quote: ... DNA was isolated according to Monarch® Plasmid Miniprep Kit (BioLabs, USA) and the PureYield® Plasmid Midiprep Protocol System (Promega ...
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bioRxiv - Genetics 2023Quote: Plasmids were constructed using Gibson assembly (New England BioLabs Cat. No. E2611), as follows ...
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bioRxiv - Plant Biology 2023Quote: ... The plasmid construct was assembled using Gibson cloning58 (E5510S, New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Plasmid was then constructed using an NEB T4 Ligase protocol (NEB – m0202L) and transformed into competent bacteria (DH5α ...
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bioRxiv - Biochemistry 2023Quote: ... and the resultant plasmids were transformed in E.Coli BL21-DE3 cells (NEB).
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 DNA fragment plasmids were digested with I-SceI (NEB) to release the overlapping fragments from the backbones ...
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bioRxiv - Biochemistry 2023Quote: ... Each plasmid was then digested with EcoRI-HF and BamHI-HF (NEB) restriction enzymes ...
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bioRxiv - Systems Biology 2023Quote: ... the plasmid was digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and SacI-HF (New England Biolabs ...
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bioRxiv - Biophysics 2023Quote: ... and simultaneous blunt ligation of the plasmid using T4 DNA ligase (NEB). For the N-terminal ybbR-mutant the tag was inserted between E15 and E16 in the β’-subunit ...