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Citations for New England Biolabs :
251 - 300 of 8761 citations for Mouse Melanocyte Stimulating Hormone Receptor MC1R ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: Libraries were constructed using NEBNext Ultra RNA Library Prep for Illumina kits (New England Biolabs kits E7490 and E7530), as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... a ChIP-seq library was made using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit (NEB).
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bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were quantified by qPCR using a library quantification kit (NEBNext® Library Quantification Kit, E7630, New England Biolabs). Illumina sequencing was performed on a HiSeq2500 instrument for 100 single end read cycles (Genome Sequencing Core ...
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bioRxiv - Genomics 2023Quote: ... Final immunoprecipitated DNA samples from the kit were then purified using a Monarch PCR and DNA cleanup kit (5μg) (NEB), with a final elution volume of 50μL in TE buffer ...
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bioRxiv - Cell Biology 2019Quote: ... then incubated with either mouse anti-MBP antibodies at a dilution of 1:5000 (New England Biolabs) or 1:2500 mouse anti-GFP antibodies (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... The mouse C10 G54R mutation removes a cut site of the restriction endonuclease BanII (NEB, cat# R0119S), introducing a restriction fragment length polymorphism ...
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bioRxiv - Cell Biology 2023Quote: ... DNA isolated from lysed mouse aortic SMCs was digested with enzyme cocktail containing HindIII – XbaI – EcoRI (NEB). Following digestion ...
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bioRxiv - Cancer Biology 2023Quote: Mouse NIK coding sequence in the pENTR shuttle plasmid was mutated using Phusion high fidelity polymerase (NEB) and oligonucleotides designed to carry the desired mutations (indicated by lowercase letters) ...
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bioRxiv - Biochemistry 2023Quote: ... Bound protein was detected by western blot using mouse anti-MBP diluted 1:10,000 (New England Biolabs), fluorescent secondary antibodies (Li-Cor Biosciences) ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons from mouse embryo lysates were generated using Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 25 μL reaction ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNA samples isolated by PureLink Mini kit were subsequently purified by Magnetic mRNA Isolation kit (New England Biolabs, USA). mRNA concentration was estimated by Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... amplicons were gel purified with the InnuPREP Gel Extraction Kit (Analytik Jena) or the Monarch DNA Gel Extraction Kit (NEB), and submitted for Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted using the Qiagen RNeasy Micro kit and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs). All libraries were sequenced on an Illumina HiSeq 2500 with 100 bp single-end reads.
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bioRxiv - Molecular Biology 2021Quote: ... Adaptor and index primers were added to the sheared DNA fragments using a next-generation sequencing library preparation kit (NEBNext Ultra DNA Library Prep Kit for Illumina; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted using illustra RNAspin Mini isolation kit (Cytiva) or Monarch Total RNA Miniprep Kit (New England Biolabs) subsequent to spheroblast construction using 10 U of Zymolyase (ZYMO Research ...
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bioRxiv - Developmental Biology 2019Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II library prep kit with the NEB Unique Dual Index primer set kit (New England Biolabs). Samples were sequenced at the Northwestern University NuSeq Core Facility on an Illumina HiSeq 4000 on single-end 50 bp mode ...
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bioRxiv - Microbiology 2019Quote: ... using Agilent High Sensitivity DNA kit in a Bioanalyzer 2100 instrument then quantitated using qPCR based NEBNext Library Quant Kit for Illumina (New England Biolabs) with a Piko-Real Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA libraries were generated using NEBNext rRNA Depletion kit combined with NEBNext Ultra II Direction RNA Library Prep kit for Illumina (New England Biolabs). We used 100 ng of starting RNA material for input and pre-sorted synaptosomes and 1-5 ng for vGLUT1+ sorted synaptosomes which corresponds to ~100 Mio synaptosomes collected per P3 fractions ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples with RIN value higher than 8 were processed using the NEBNext poly(A) mRNA Magnetic Isolation kit and the NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR/reaction cleanup and gel extraction were conducted with the Monarch PCR and DNA cleanup kit and Monarch DNA gel extraction kits (NEB). E ...
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bioRxiv - Microbiology 2021Quote: ... cholerae and phages was extracted using commercially available kits (Qiagen DNeasy Blood and Tissue or Monarch genomic DNA purification kit (New England BioLabs). For preparation of phage DNA ...
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bioRxiv - Microbiology 2022Quote: ... After enrichment of mRNA using the Universal Ribodepletion Kit cDNA preparation was performed with the Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq 3000 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... 60 ng of total RNA was used for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext rRNA Depletion Kit (New England Biolabs). The libraries were paired-end sequenced (2 × 75 bp ...
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All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cellsbioRxiv - Neuroscience 2021Quote: ... and RNA was consecutively isolated using a column based RNA isolation kit according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit; #T2010S New England Biolabs). Strand specific cDNA library preparation from polyA enriched RNA (150 bp mean read length ...
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bioRxiv - Immunology 2019Quote: ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.
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bioRxiv - Systems Biology 2021Quote: Bulk RNA-seq libraries were prepared from 1000 ng of RNA using the NEBNext Ultra RNA Library Prep kit for Illumina or the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enrichment of mRNA was done using the Universal Ribodepletion Kit followed by Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq™ 2500 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed from 1 µg of total RNA using Illumina’s TruSeq stranded mRNA Library Preparation Kit or from 500 ng total RNA using NEBNext Ultra II RNA -polyA+ (mRNA) Library Preparation Kit (New England Biolabs) following the manufacturer’s description.
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bioRxiv - Genetics 2021Quote: ... RNA samples were depleted of rRNA using the QIAseq FastSelect RNA Removal Kit then processed into libraries using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Samples were recovered by phenol/CHCl3/isoamyl alcohol extraction twice and purified as per kit instruction using Monarch PCR and DNA cleanup Kit (New England BioLabs). DNA was suspended in 200 ul pre-warm (55°C ...
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bioRxiv - Developmental Biology 2021Quote: ... or deletions in full-length Gpr161 were generated using Quikchange site-directed mutagenesis kit (Stratagene or Q5 Mutagenesis Kit (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... RNA-sequencing libraries were prepared with a NEBNext rRNA Depletion Kit and an ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). Then ...
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bioRxiv - Genomics 2022Quote: MNase-seq libraries were prepared from purified nucleosomal DNA using the kit NEBNext Ultra DNA Library Prep Kit for Illumina (cat. # E7370L New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: RNA was purified by silica-based SPE using Monarch RNA Cleanup Kits (for in vitro assays) or Total RNA Miniprep Kit for intracellular samples (NEB).
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Qiagen RNeasy Plant Mini Kit and cDNA was synthesised using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs). SYBR Green Power Up (Thermofisher ...
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bioRxiv - Plant Biology 2023Quote: ... from 100 to 1000 ng of total RNA using a stranded kit (Illumina TruSeq Stranded Total RNA Kit or NEB Next Ultra™ II Directional RNA Library Prep Kit for Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription was performed with either HiScribe T7 ARCA mRNA kit or the HiScribe In Vitro Transcription Kit (NEB) according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... libraries were prepared either with Nugen Ovation Ultralow V2 DNA-Seq library prep kit (NuGen) or NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Capped RNA was synthesized from the pCS2+hSpCas9 vector using an AmpliCap SP6 High Yield Message Maker Kit (Cell Script) and then purified with a Monarch RNA Cleanup Kit (50 µg) (New England BioLabs).
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bioRxiv - Neuroscience 2023Quote: RNA was extracted using the QIAzol and the NEB Monarch RNA extraction kit and ribosomal RNA was depleted using the NEBNext rRNA depletion kit (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Illumina Ribo-Zero Plus rRNA Depletion kit followed by library preparation with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... was extracted via the UHMW DNA extraction protocol of the Nanobind CBB Big DNA kit (Circulomics) or via the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs) and quantified using Qubit dsDNA Broad Range kit (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB). Following purification ...
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bioRxiv - Microbiology 2024Quote: ... The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB, China) for RNA and DNA samples ...
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bioRxiv - Cancer Biology 2021Quote: ... Ligations were performed using Quick Ligation Kit (NEB). Reaction mixes were incubated for 5 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... using Quick Ligation Kit (NEB; Cat. No. M2200L). The ligated DNA was PCR amplified with primers containing Illumina paired-end sequencing adaptors ...
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bioRxiv - Developmental Biology 2021Quote: ... we used the EnGen sgRNA synthesis kit (NEB) following the manufacturer’s instructions with the following targeting sequences tnpo1 sgRNA#1 (5’-GGCATGGGGGCCACCTCTTG-3’) ...
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bioRxiv - Developmental Biology 2021Quote: ... NEBNext Ultra II DNA Library Prep Kit (NEB) with 11 cycles of PCR was used to prepare sequencing libraries ...