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Citations for New England Biolabs :
351 - 400 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... These plasmids were linearized with Pme I (New England Biolabs) and transformed in strain DPY034 for integration at the TRP1 locus to obtain strains DPY1805 and DPY1818 ...
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bioRxiv - Microbiology 2021Quote: ... The N expression plasmid was linearized using EcoRV-HF (NEB). Capped-RNA was produced using the mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NheI (New England BioLabs) between the end of KDM5B and the MS2 stem loops ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NheI (New England BioLabs) between the end of KDM5B and beginning of SunTag ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NotI (New England BioLabs) at the beginning of SM ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with AscI (New England BioLabs) between the end of Kif18b and beginning of SM ...
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bioRxiv - Cell Biology 2020Quote: The parental plasmid was digested with the DpnI enzyme (NEB). The mCherry was then replaced by eGFP to generate the RUSH CD63-YA-eGFP plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... Whole-plasmid amplification reactions were performed using Phusion polymerase (NEB) and Ampligase (Epicentre) ...
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bioRxiv - Synthetic Biology 2021Quote: ... treatment and DNA purification with Monarch Plasmid Miniprep kit (NEB), or (ii ...
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bioRxiv - Microbiology 2020Quote: ... replicon plasmid DNA linearized with AscI (NEB, Ipswich, Massachusetts, USA) was used to template T7 in vitro transcription reactions ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid construction was carried out using enzymes obtained from NEB (NEBuilder HiFi DNA assembly master mix ...
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bioRxiv - Microbiology 2021Quote: ... of existing plasmids or HiFi Gibson Assembly (New England Biolabs) of linear dsDNA fragments ...
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bioRxiv - Genetics 2020Quote: ... ESCs were electroporated with the linearized plasmid (Fsp1 NEB R0135) in the presence of hygromycin and individual clones were isolated as we have done previously and expanded for further experiments.
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bioRxiv - Genetics 2020Quote: ... Ligated plasmids were transformed into NEB High Efficiency (NEB, C2987) bacteria ...
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bioRxiv - Molecular Biology 2021Quote: ... The pLentiGuide-Blast plasmid was digested with BsmBI-V2 (NEB) at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen) ...
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bioRxiv - Genomics 2022Quote: pUC19 plasmid was digested with SmaI (New England Biolabs, R0141S) and the linearized plasmid was gel extracted with the QIAquick Gel Extraction Kit (Quiagen ...
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bioRxiv - Microbiology 2022Quote: ... The minipreped plasmids were cut by HindIII (NEB cat. R3104S), and gel electrophoresed.
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bioRxiv - Plant Biology 2022Quote: ... and introduced into binary plasmids using NEBuilder Hifi assembly (NEB) (Figure 1a) ...
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bioRxiv - Immunology 2022Quote: ... plasmids were linearized with PstI (New England Biolabs, MA, USA) and purified using a PCR purification kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was first linearized by EcoRV (New England Biolabs), and then transfected into VSG2-expressing cells (2T1) ...
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bioRxiv - Molecular Biology 2023Quote: ... The parental plasmid was digested with 5U DpnI (NEB, R0176S). Plasmid transformation was achieved by using the heat shock method (42°C ...
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bioRxiv - Biophysics 2023Quote: ... Plasmids were linearized using Nhe I HF (New England Biolabs) and transcribed in vitro (mMessage-mMachine T7 Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... The final donor plasmid was generated through Gibson cloning (NEB) of a synthetic FseI-HA-KRAB-AscI DNA fragment (GenScript ...
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bioRxiv - Cell Biology 2023Quote: ... we linearized the plasmid for genomic integration using AflII (NEB), a silent mutation inserted previously.
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bioRxiv - Bioengineering 2023Quote: ... The Csy3-VPR-Csy4 plasmid was digested with MluI (NEB) and BamHI (to remove the VPR TAD ...
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bioRxiv - Immunology 2023Quote: ... plasmids using T4 DNA ligase (New England BioLabs, cat. M0202S). Sanger sequencing confirmed successful cloning.
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bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Biophysics 2023Quote: ... We digest the plasmid with Hind III (New England Biolabs) to generate a linear template ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmids were verified by digestion by restriction endonucleases (NEB). Plasmid mapping and sequencing alignments were carried out using SnapGene software (GSL Biotech ...
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bioRxiv - Genomics 2023Quote: ... 46 plasmid using restriction digestion with AatII and Aflll (NEB). The library was then re-electroporated and purified as above.
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bioRxiv - Microbiology 2023Quote: ... plasmid DNA encoding rat HEV was linearized using EcoRI (NEB). Five percent of the reaction was subjected to gel electrophoresis with ethidium bromide staining and visualized with ultraviolet light to verify that linearization had occurred.
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bioRxiv - Genomics 2023Quote: ... Dual-enSERT-2.1 plasmid was digested with NotI (NEB, R3189) and AgeI (NEB ...
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bioRxiv - Microbiology 2023Quote: ... the plasmids were linearized using EcoRV endonuclease (New England Biolabs). cRNA synthesis was accomplished through in vitro transcription using the HiScribe T7 ARCA mRNA Kit with tailing (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... Gel extraction and plasmid preparation kits were purchased from NEB and Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: We digested the resulting plasmids by NotI-HF® (NEB) and inserted cassettes expressing fusions of antibiotic resistance genes with deltaTK downstream of the lox66 sites by Gibson assembly ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were cloned and propagated in NEB Turbo cells (NEB) — except for pDonorCI derivatives ...
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bioRxiv - Biophysics 2023Quote: Plasmid pUC19 was linearized by using restriction enzymes HindIII (NEB) and XbaI (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids bearing mutations were cut with AseI (NEB, Catalog # R0526S) and SspI (NEB ...
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bioRxiv - Genomics 2023Quote: ... We digested the plasmid pRS31N with SacII (New England Biolabs), treated the digestion with calf intestinal alkaline phosphatase (CIP ...
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bioRxiv - Genetics 2023Quote: ... pAAVsc-JeT-MCOLN1-pA plasmid was digested with EcoRI (NEB) to recover the ITR-flanked AAV genome to be used as standard ...
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bioRxiv - Synthetic Biology 2024Quote: ... pLK35 plasmids were transformed into NEB Express Iq cells (NEB) which are a bL21 derivative that constitutively expresses the lac repressor ...
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bioRxiv - Genomics 2024Quote: ... plasmids were linearized using BamHI or NotI (New England Biolabs) and in vitro-transcribed using T7 or SP6 polymerase (DIG RNA Labeling Mix ...
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bioRxiv - Cell Biology 2023Quote: ... and the plasmid was amplified using Q5 DNA polymerase (NEB). The template plasmid was digested with DpnI (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmids were transformed into DH5a competent cells (New England Biolabs) on LB agar plates with ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... Before transfection pKD plasmids were digested overnight with EcoRV (NEB). The enzyme was then subjected to heat inactivation for 20 min at 65 °C and then mixed with the pUF1-Cas9 plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg of replicon plasmid was linearised with AscI (NEB), purified by phenol-chloroform extraction ...
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bioRxiv - Biophysics 2024Quote: ... The plasmid was linearized with NheI-HF (New England Biolabs) and purified using QIAquick PCR Purification Kit (QIAGEN) ...
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bioRxiv - Microbiology 2024Quote: ... Isolation was performed using Monarch Plasmid Miniprep Kit (NEB, UK).
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bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-MBP (NEB, E8032). Peroxidase and Alexa-fluor conjugated secondary antibodies were purchased from Jackson Laboratories and ThermoFisher ...