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Citations for New England Biolabs :
651 - 700 of 1299 citations for Mouse Anti Hepatitis C Virus NS4b Antibody 1858 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 25 °C) using a custom methionine(-) Flexible In vitro Translation system composed by PURExpressTM (ΔRF123) Kit (New England Biolabs) solution B ...
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bioRxiv - Plant Biology 2020Quote: ... and pan-anti-acetyllysine (pan-anti-Kac, PTM Biolabs, China, Catalog No. PTM-101) were used in this study ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated with custom-generated anti-AcK142 and anti-AcK226 Abs (EZ BioLabs) at a dilution of 1:200 in PBST for 1 h at 37 ⁰C ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-Snail (NEB, 3879S). Secondary antibodies were purchased from CST ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP (NEB E8032) antibodies (all diluted 1:10,000 in TBST-5% non-fat milk powder).
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bioRxiv - Physiology 2022Quote: ... anti-acetyl-lysine (PTM Biolabs), and anti-tubulin antibodies.
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bioRxiv - Neuroscience 2022Quote: ... anti-HA (Cell Signaling (NEB) 3724S ...
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bioRxiv - Molecular Biology 2022Quote: ... rabbit anti-DYKDDDDK (#D6W5B; NEB) and rabbit anti-myc (#71D10 ...
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bioRxiv - Synthetic Biology 2022Quote: ... rabbit anti-SNAP-tag (NEB; P9310 ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA (catalog # 3724S, NEB), anti-PRG-1 (33) ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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bioRxiv - Genomics 2022Quote: For each of the two ligation-based protocols used for mouse sperm (Truseq, NEB Next), two replicate libraries for mouse sperm were prepared with the additional condition of an 18 hour ligation at 16°C for the ligation of the 3’ adapter in the attempt to increase ligation efficiency ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...
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bioRxiv - Cancer Biology 2023Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat; NEB, E6310X). rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Bioengineering 2020Quote: Antibodies were first digested with PNGase F (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and MBP was detected with MBP antibodies (New England Biolabs).
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bioRxiv - Plant Biology 2020Quote: ... 2 µg of RNA for each sample was treated with DNase I at 37°C for 10 minutes (New England Biolabs) and 0.5 µg was used for reverse transcription with the GoScript Reverse Transcription Mix ...
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bioRxiv - Biophysics 2021Quote: ... The AcrIIA4 expression vector was modified to express AcrIIA11 with a C-terminal NLS and HA tags using the HiFi Assembly Kit (NEB).
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bioRxiv - Biochemistry 2021Quote: ... RNA integrity was assessed by performing 1% TBE agarose gel electrophoresis with samples that had been boiled for 95°C for 5 min in RNA loading dye (New England Biolabs). Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... with primers to introduce BamHI and NotI sites and cloned into pcDNA4/TO-3xFLAG (C-terminal tag) using T4 DNA ligase (New England Biolabs). pcDNA4/TO-ORF24 202-752-3xFLAG (Addgene #138424 ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 15,000 x g for 30 min at 4°C and the clarified extract was loaded onto a chitin resin (NEB) column pre-equilibrated with column buffer for purification at RT ...
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bioRxiv - Genetics 2021Quote: ... which was followed by 37°C for 30 min with addition of 50ul 10% Triton-X 100 to quench SDS and 37°C overnight with addition of 40ul AluI (R0137L, NEB) total ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were individually treated for 16 h at 37 °C with 20 units of DpnI enzyme (New England Biolabs). Following gel extraction using Wizard SV Gel and PCR CleanUp System (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated overnight at 37 °C in Hybridization Buffer (10% Formamide, 10% 20x SSC, 400 µg/ml E. coli tRNA (New England Biolabs), 5% dextran sulfate ...
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bioRxiv - Developmental Biology 2020Quote: ... TA overhangs were added by incubating the insert for 10 min at 72 °C with 50mM DNTPs and Taq polymerase (NEB), in order to perform TA cloning into a PCR4 TOPO vector (ThermoFisher) ...
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bioRxiv - Genomics 2020Quote: ... A 20 μL aliquot of DNA was repaired (15 min, 25 °C) in a 40 μL reaction using T4 DNA polymerase (New England Biolabs). After purifying the DNA (MinElute™ Reaction Cleanup Kit ...
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bioRxiv - Biochemistry 2022Quote: ... trcP or fragments of the trcP ORF were cloned into pHL100’s SmaI site with the 23 nt upstream sequence and the Flag tag sequence on the C-terminal using NEBuilder HiFi DNA Assembly Master Mix (NEB); subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... human emerin was first fused to the C-terminus of a SNAP tag by AscI and XhoI insertion in a pSNAP-tag(m) plasmid (NEB). SNAP-emerin was then subcloned into a modified pFUW lentiviral vector by NheI and AgeI insertion ...
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bioRxiv - Biochemistry 2022Quote: ... a cysteine was added to the N-terminus of the coding sequence directly after the precision protease recognition site or after the six-histidine tag on the C-terminus using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: Micro-C sequencing libraries were generated by using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) with some minor modifications ...
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bioRxiv - Genomics 2020Quote: ... Primary restriction enzyme digestion of intact nuclei was carried out overnight at 37°C using 50,000 U of DpnII (NEB: #R0543) with agitation at 900 RPM ...
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bioRxiv - Molecular Biology 2020Quote: ... then blunted overnight at 37°C in 100 μl NEBuffer 2 with 0.1 mM dNTPs and 1 μl Klenow (NEB M0210S). After rinsing twice with 1 ml tris buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 40 μl 50% PEG 8000 for 1 hour at room temperature then incubated overnight at 25°C in 100 μl 1x T4 RNA ligase buffer (NEB) containing 40 μl 50% PEG 8000 ...
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bioRxiv - Molecular Biology 2020Quote: ... were separately subjected to restriction enzyme double digestion at 37 °C overnight in 20 μL volumes containing 20 U BamHI-HF (cat. no. R3136S, New England BioLabs), 20 U EcoRI-HF (cat ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were treated with Klenow exo- at 37 °C for 30 min to fill the ends of adaptors and then trimmed with T4 DNA polymerase (NEB) at 12 °C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... after incubating the samples with 2 μl of RNase A (Thermo) at 37 °C for 2h and then with 8 μl of proteinase K (NEB) at 55 °C for 4h ...
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bioRxiv - Molecular Biology 2021Quote: ... left to air-dry and then digested overnight at 37°C in 50 μl TE with 20 U EcoRI-HF (NEB). Samples were extracted with 50 μl phenol:chloroform ...
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bioRxiv - Molecular Biology 2020Quote: ... in a final volume of 50 μl for 1 h at 37°C and 1X T4 of RNA Ligase Reaction Buffer (NEB, 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by the insertion of a DNA fragment containing C-terminal 2×HA tag by NEBuilder HiFi DNA Assembly Master Mix (NEB). Finally ...
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bioRxiv - Genomics 2019Quote: ... 400 ng of purified DNA was ligated overnight at 16 °C to adapters (Table S4) with T4 DNA ligase (NEB). Ligated DNA was purified using MiniElute columns (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... and msDNA extracts were incubated overnight at 37°C with 0.5 units/μL of truncated RecE (NEB; catalogue number M0545S). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM EDTA) at 65°C for 15 minutes and later the proteins were digested by 10U Proteinase K (NEB) at 37°C for 30 minutes ...
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bioRxiv - Genomics 2019Quote: ... we digested each sample with the restriction enzymes SphI-HF and MluCI for 1 hour at 37°C (New England Biolabs). These enzymes were chosen because they are insensitive to DNA methylation (and therefore will not show biased template enrichment ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ...
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bioRxiv - Cancer Biology 2020Quote: ... branched DNA (which can be a consequence of the RCA) was resolved by a 1 hour incubation at 37°C with 4 μl T7 endonuclease (New England Biolabs) and re-purified using AMPure beads ...
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bioRxiv - Molecular Biology 2019Quote: ... The reactions were incubated for 60 minutes at 37 °C and terminated by the addition 2 μL of Proteinase K (NEB) which had been supplemented with 0.1 reaction volume of 1 M Tris-HCl pH 8.0 ...
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bioRxiv - Genomics 2020Quote: ... at 37°C overnight and purified digests were ligated overnight at 16°C using T7 DNA ligase as per manufacturer’s instructions (NEB M0318). Ligation reactions were transformed into One Shot™ Stbl3™ chemically-competent E.coli cells (42°C heat-shock ...
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bioRxiv - Biochemistry 2019Quote: ... Precipitated proteins were removed by centrifugation at 14000g for 30 min at 4°C and the remaining soluble protein was incubated with enterokinase light chain (NEB) for 16h at room-temperature as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... DpnI treatment was performed for 15 min at 37°C and the four different amplified parts were purified and then digested with BsmBI (NEB) enzyme at 55 C for 1.5 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... washed once with ice-cold NETN150 buffer and digested for 12 min at 37 °C with 50 gel units of micrococcal nuclease (NEB) per milligram of DNA in NETN150 buffer containing 5 mM CaCl2 ...