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Citations for New England Biolabs :
301 - 350 of 1299 citations for Mouse Anti Hepatitis C Virus NS4a Antibody 1866 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 40 µg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
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bioRxiv - Microbiology 2019Quote: ... Incubation for 1 h at 55°C with 8U of proteinase K (NEB P8107S) finally disrupted viral capsids ...
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bioRxiv - Genomics 2020Quote: ... DNA was digested for 3h at 37°C with Dpn II (New England Biolabs) and heat inactivated at 65°C for 20 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... as described below) was digested at 37 °C overnight using PstI restriction enzyme (NEB) in NEBuffer 3.1 (100 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... cells recovered for three hours at 37°C in SOC media (NEB, cat. # B9020S). An aliquot was then taken to titer transformants and the remaining cells (860 µl ...
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bioRxiv - Biochemistry 2020Quote: ... The amplified backbone was treated with Dpn1 for 16 hours at 37°C (NEB) followed by a purification using a spin column ...
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bioRxiv - Genomics 2019Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction was performed at 37° C in Smart buffer (New England Biolabs, USA) in a Real-time PCR machine (Rotor-Gene Q ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, 1 h 50° C) into backbones previously digested with corresponding restriction enzymes (NEB ...
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bioRxiv - Genomics 2022Quote: ... cells were digested overnight at 37°C using 50U of MboI (NEB, Cat. #R0147). After biotin filling ...
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bioRxiv - Microbiology 2023Quote: ... gene marker cassette by overnight ligation at 4 °C with T4 DNA Ligase (NEB). Overnight ligations were transformed into chemically competent DH5ɑ E ...
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bioRxiv - Microbiology 2023Quote: ... Ligation was performed overnight at 4 °C using T4 DNA ligase (New England Biolabs) followed by heat-inactivation for 10 min at 80 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Backbones were linearized with PCR or with restriction enzymes (NEB, 1h at 37°C). PCR-amplified or digested products were purified (Monarch PCR & DNA Cleanup Kit ...
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bioRxiv - Genomics 2023Quote: ... and 30% for overnight at 4 °C) with RNase inhibitor [New England Biolabs (NEB), M0314L ...
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bioRxiv - Biochemistry 2023Quote: ... activation was performed overnight at 4 °C with 5 μg of Factor Xa (NEB) per mg of protein ...
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bioRxiv - Developmental Biology 2023Quote: ... ∞ at 4 °C) using NEBNext HighFidelty 2x PCR Master Mix (New England Biolabs, M0541S) and Illumina i5 and i7 indices54 ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-myc (New England Biolabs, clone 9B11, 1:2,000), rabbit α-pol-I largest subunit 15 (1:100 ...
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bioRxiv - Genetics 2024Quote: ... and mid-range PFG ladder (for mouse samples) (NEB Inc.) and 1 kb ladder (GeneDirex Inc.) ...
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bioRxiv - Microbiology 2019Quote: PCR mixtures were digested for 1 h at 37 °C with DpnI (New England Biolabs) to remove the template plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... Aβ40 fibrils were digested using the Endoproteinase Lys C enzyme (New England BioLabs, Ipswich, MA) at a 1/50 (w/w ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was digested with DpnI for 3h at 37°C (New England Biolabs) and then 5 µl was transformed into Stbl3 competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... the sample was ligated overnight at 16°C using T4 DNA ligase (New England BioLabs).
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bioRxiv - Microbiology 2021Quote: ... The C-tailing reaction was performed by using terminal transferase (TdT) buffer (New England Biolabs), CoCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... for 16 cycles annealing at 67 °C was mixed with 1 μL of ExoI (NEB) digested mixture as the template ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated at 37 °C for 2 hours in 1x T4 DNA ligase buffer (NEB). Following this incubation 1200 units T4 DNA ligase (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Restriction digest was performed overnight at 37°C (950 rpm) using 800 units MboI (NEB) per 5 million cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were digested for 15 min at 37 °C with 5 μL of ExoI (NEB) to remove first round primers ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 hr at 37 °C and Proteinase K (4 U; P8107S, NEB, Ipswich, MA) for another 2 hr at 55 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Nicking was performed according to the manufacturer’s instructions for 60 min at 50 °C (NEB) and the product was purified using a PCR purification kit (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were then incubated at 30°C for 30 minutes with lambda protein phosphatase (NEB).
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bioRxiv - Biochemistry 2022Quote: ... Mif2 was treated for 2 h at 30 °C with lambda-phosphatase (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... periplasmic expression vectors with C-terminal HA-His6 tags using Gibson cloning (New England Biolabs). Nanobodies were produced in Escherichia coli WK6 transformed with the respective expression vectors ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 30 min and dephosphorylated by 1U of Shrimp Alkaline Phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... per 1µg protein for 1 hour at 30°C in PMP buffer (New England Biolabs) plus 1mM MnCl2 ...
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bioRxiv - Genetics 2020Quote: ... Phusion DNA polymerase with annealing at 63°C was used according the manufacturer’s instructions (NEB). Both products were sequenced with DG1328-DG1333 using Sanger sequencing performed by the Australian Genome Research Facility.
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bioRxiv - Microbiology 2019Quote: ... 72°C for 60 seconds) in 50 µl reactions using Phusion PCR polymerase mix (NEB) containing 25 pmol each of the following ultramers (the protospacer-matching sequence is underlined) ...
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bioRxiv - Biochemistry 2021Quote: C-terminally (His)6-tagged LukE was expressed in BL21 (DE3) Escherichia coli cells (NEB). Transformed cells were grown at 37 °C in Terrific broth supplemented with 100 μg/mL ampicillin to a density of OD600 = 0.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... digested genomic DNA by O/N incubation at 16°C with T4 DNA ligase (NEB). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... acylated dasTMPRSS2 was treated with 50 U PNGase F (NEB; 37 °C for 45 min) to trim N-glycan branches ...
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bioRxiv - Developmental Biology 2021Quote: ... and the fragments were ligated over night at 4°C with T4 DNA Ligase (NEB) and heat shock transformed into DH5α competent E ...
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bioRxiv - Immunology 2020Quote: ... transferred to 42°C and digested by immediate addition of 2ul of beta-agaraseI (NEB) per 300ul of melted gel and incubation at 42°C for 1h ...
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bioRxiv - Immunology 2022Quote: ... cells were digested with Micrococcal Nuclease at 37°C for 40 min (New England BioLabs) and added 10 mM EDTA to stop the reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... for 30 minutes at 37°C in 1X rCutSmart Buffer (New England Biolabs, cat# B6004S), followed by Trizol extraction ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligated over night at 16°C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Immunology 2023Quote: In situ Hi-C experiments were performed as previously described2 using restriction enzyme MboI (NEB) with minor modifications ...
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bioRxiv - Plant Biology 2022Quote: ... The digestion buffer (25mM triethylammonium bicarbonate (TEAB)) contained the Lys-C protease (P8109S, NEB, Australia) at a 1:20 protease to protein ratio ...
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...
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bioRxiv - Biochemistry 2023Quote: ... reactions were conducted at 37 °C using the PURExpress System (New England Biolabs, Ipswich, MA) 111 in the absence or presence of increasing concentrations of ABCF protein ...
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bioRxiv - Cancer Biology 2023Quote: ... A negative control treated for 4 hours at 37 °C with RNaseH1 (New England Biolabs) was included for each condition ...
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bioRxiv - Cancer Biology 2023Quote: ... digested overnight at 37°C with 1 μl 20 U/μl NotI-HF (NEB R3189S) and 1 μl 10 U/μl PmeI (NEB R0560S ...