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Citations for New England Biolabs :
401 - 450 of 1299 citations for Mouse Anti Hepatitis C Virus NS3 Antibody 1828 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Hi-C single-index library preparation was performed as previously described56 using MboI (New England Biolabs) restriction enzyme.
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bioRxiv - Microbiology 2023Quote: ... Total RNA was then digested using DNase I for 2h at 37°C (New England Biolabs) to remove residual plasmid DNA and then purified using Monarch RNA cleanup kit (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs) plus 1 mM MnCl2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the P1-adaptors and Sbf I-HF (New England Biolabs)-digested DNAs for 90 min at 37°C were ligated by T4 ligase (New England Biolabs). After DNA pooling ...
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bioRxiv - Cell Biology 2023Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology)6 sequence in-frame with the KRT5 sequence separated by a short peptide linker (DPAFLY) ...
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bioRxiv - Cell Biology 2023Quote: ... 45-72°C for 30 seconds (depending on melting temperature of primer pairs determined by NEB Tm calculator https://tmcalculator.neb.com) ...
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bioRxiv - Genomics 2024Quote: ... The Hi-C libraries were amplified for 11–15 cycles with Q5 master mix (NEB, M0492L) following the operation manual ...
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bioRxiv - Genomics 2024Quote: ... followed by proteinase K treatment (New England Biolabs, #8102S 20µg/µL, 1.5 h at 55°C). DNA was eluted in Milli Q water ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed with TBS-T and then incubated with an anti-rabbit horseradish peroxidase conjugated secondary antibody (New England Biolabs, 1:10,000 in 5% skim milk in TBS-T) for 2h ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H3 (Lys 23) mouse mAb (PTM Biolabs, SKU: PTM 307), Butyryl-Histone H4 (Lys 8 ...
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bioRxiv - Genomics 2020Quote: Mouse NIH/3T3 and human Jurkat DNA were from NEB (Ipswich, MA), and XP12 phage DNA was obtained from Dr ...
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bioRxiv - Molecular Biology 2022Quote: ... we used NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7405), NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765 ...
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bioRxiv - Genetics 2023Quote: ... using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB E7405) or the NEBNext Poly(A ...
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bioRxiv - Biochemistry 2023Quote: ... custom-made rabbit polyclonal anti-AcK142 or anti-AcK226 (Ez Biolabs) Abs were incubated overnight at 4 °C with the lysate from mock vs ...
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bioRxiv - Microbiology 2021Quote: ... Ligation to each splint was performed overnight at 16°C using T4 DNA ligase (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then incubated at 45 °C for 2 hr with 5 μL of Proteinase K (NEB) in the presence of 40 mM EDTA (pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Digested chromatin was then ligated at 16 °C with 10000 U of T4 DNA ligase (NEB, M0202) in ligase buffer supplemented with 10% Triton-100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... padlock probe ligation was performed overnight O/N at 25°C using the SplintR ligase (NEB, M0375) at a final concentration of 0.5 Units/μl ...
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bioRxiv - Microbiology 2019Quote: ... and phosphorylated for 30 minutes at 37 °C with 4U of T4 Polynucleotide Kinase (New England Biolabs) in a total volume of 20 µl according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... All Gibson reactions were performed at 50 °C for one hour with T5 exonuclease (New England Biolabs), Phusion polymerase and Taq ligase (New England Biolabs) ...
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bioRxiv - Bioengineering 2019Quote: ... an mCherry ORF was fused to the C-terminus of each Acr by HiFi DNA assembly (NEB). To insert 3xmiR122 MREs in the 3’ UTR of each Acr ...
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bioRxiv - Biochemistry 2021Quote: ... and C-terminal additions) were assembled with the pcDNA5 backbone using Hi-Fi DNA assembly (NEB; E2621S).
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bioRxiv - Biochemistry 2020Quote: ... treated with lambda phosphatase protein after purification at 30°C for 1 hr (New England BioLabs, P0753L), or co-expressed in BL21 cells with RFP-lambda phosphatase ...
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bioRxiv - Biochemistry 2020Quote: ... The second aliquot was incubated overnight at 37 °C with β1-4 galactosidase (New England Biolabs #P0745) using the same reaction conditions as the neuraminidase above ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was digested for 1 hour at 37°C with NcoI (New England BioLabs, Ipswich, MA) unless otherwise specified in a final volume of 20 μl before evaluating restriction fragment patterns by 1% agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... 14 μg of sample DNA were digested overnight at 25°C with CviQI restriction enzyme (NEB, R0639L), which cuts outside the region of interest.
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bioRxiv - Cell Biology 2021Quote: ... DNA was transformed and propagated in NEB stable competent cells cultured at 30°C (New England BioLabs). The resulting DNA plasmids encoding each ERS tail fused to Gaussia luciferase with a MANF signal peptide were sequence verified for proper ligation of the tail.
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bioRxiv - Developmental Biology 2022Quote: ... They were then incubated for 20 min at 25°C with the SNAP-Cell TMR-Star (NEB) at a concentration of 2 μM in Shields and Sang M3 Insect cell complete medium ...
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bioRxiv - Cell Biology 2022Quote: ... was digested for 4 h at 37 °C using the restriction enzyme BbsI (#R3539S, New England Biolabs) and run on a 1 % agarose gel for 3.5 h at 100 V ...
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bioRxiv - Biochemistry 2020Quote: ... Fragmentation of 1 µg mRNA aliquots for 5 min at 94°C in 1x Fragmentation Buffer (NEB) was optimal to obtain a pool of 100-200 nt fragments ...
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bioRxiv - Pathology 2022Quote: ... for 15 min at 37°C and subsequently digested with TaqI restriction enzyme (100U, New England Biolabs) at 65°C for 1 h to remove single strands ...
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bioRxiv - Genetics 2020Quote: ... was destroyed by incubating the reactions with Exonuclease I at 37°C (New England Biolabs, MA. USA). After exonuclease inactivation at 80°C ...
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bioRxiv - Molecular Biology 2019Quote: ... primer annealing at 23°C for 30 s) template DNA was mixed with T7 RNA polymerase(NEB), 50 mM MgCl2 ...
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bioRxiv - Genomics 2020Quote: ... 500ng of genomic DNA was digested for 4h at 37°C with Dpn I (New England Biolabs) to cut methylated GATC sites ...
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bioRxiv - Genetics 2020Quote: ... chromatin was digested overnight at 37°C and 900 rpm with 400 U of NlaIII (R0125L, NEB). NlaIII was inactivated by adding SDS to a final concentration of 1.6% and incubating the mixture for 20 minutes at 65°C while shaking (900 rpm) ...
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bioRxiv - Biochemistry 2021Quote: Radiolabeling of oligonucleotides was carried out for 1 h at 37 °C using T4 polynucleotide kinase (NEB), 0.25 μM oligonucleotide ...
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bioRxiv - Biochemistry 2021Quote: ... the denatured samples were incubated at 37 °C with or without Endo Hf (P0703S, New England Biolabs) or PNGase F (P0704S ...
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bioRxiv - Microbiology 2021Quote: ... then heat inactivated at 65°C for 30 minutes and ligated using 1 µl Quick Ligase (NEB) at room temperature for 15 minutes ...
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bioRxiv - Genetics 2019Quote: ... at 37°C for 30 minutes and then treated with 140ug of Proteinase K (NEB, Ipswhich, MA) incubating at 55°C in a thermomixer mixing at 900rpm for two hours ...
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bioRxiv - Immunology 2020Quote: ... Digested chromatin was then diluted and ligated overnight at 16°C with T4 DNA ligase (NEB, M0202M). Reversal of crosslinks ...
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bioRxiv - Molecular Biology 2021Quote: ... at 20 °C for 30 min to repair the DNA ends and then with Klenow exo- (NEB) in the NEB buffer 2 containing 0.2 mM dATP at 37 °C for 30 min for dA tailing ...
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bioRxiv - Developmental Biology 2021Quote: ... and elongation for 7 min at 72°C) using the Phusion High-Fidelity DNA Polymerase (NEB, #M0530S) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... PCR reaction according to the manufacturer’s protocol (annealing temperature: 60°C; elongation time: 15sec, 19 cycles) (NEB). Of this reaction ...
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bioRxiv - Cell Biology 2021Quote: ... mexicana genomic DNA and cloned into the pNUS C-Ter GFP NEO (pGL1132) using HiFi Assembly (NEB) to generate a complementation vector ...
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bioRxiv - Molecular Biology 2019Quote: ... ligation was performed during 4h at 22°C in the presence of truncated RNA ligase 2 (NEB)30 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and chromatin was digested for 7.5 minutes at 21°C using micrococcal nuclease (MNase) (New England Biolabs). We spiked DNA from D ...
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bioRxiv - Neuroscience 2022Quote: ... DamID adaptors were removed by digesting overnight at 37° C with 5 units of Sau3AI (R0169S; NEB). The sequencing libraries were prepared according to the Illumina TruSeq nano DNA library protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were cooled to 4°C for 30 seconds and quenched with 1.2 mM unlabeled SAM (NEB) and blotted onto Hybond-XL membrane ...