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Citations for New England Biolabs :
451 - 500 of 789 citations for Mouse Anti Cytomegalovirus Glycoprotein H gH 0861 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Lysates of HEp-2/ι1hUNGs cells mock infected or infected with wild-type HSV-1(F) at an MOI of 10 for 24 h were treated with alkaline phosphatase (CIP) (New England BioLabs) as described previously48.
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bioRxiv - Cancer Biology 2023Quote: ... Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB). The lysates were boiled and subjected to SDS-PAGE and western transfer ...
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bioRxiv - Immunology 2023Quote: ... An aliquot of 15 μl containing trimer with fluorescently labelled glycans was treated with Endo H overnight at 37°C (New England Biolabs). Glycans were extracted with a polyvinylidene-fluoride protein-binding membrane (Merck Millipore) ...
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bioRxiv - Biophysics 2023Quote: ... we digested #126-pSC-T7A1reverse-parS with SpeI-HF and BamHI-HF 1 h at 37°C and heat-inactivated for 20 min at 80°C (New England Biolabs). Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega ...
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bioRxiv - Biophysics 2023Quote: ... The 38 kbp T7A1-parS + handle construct were subsequently digested for 1 h at 37°C and heat-inactivated for 20 min at 65°C using SrfI restriction enzyme (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... concisus for 2 h at 37°C in presence of 0.4 mM S-Adenosylmethionine (SAM, New England Biolabs, Ipswich, MA). After methylation ...
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bioRxiv - Microbiology 2023Quote: ... 50 pmol of each RNA were dephosphorylated by incubation for 1 h at 37°C with 25 U of calf intestine alkaline phosphatase (NEB) in a final volume of 50 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA products were purified by using a Zymo Oligo Clean and Concentrator kit and incubated for 20 min at 37°C in the presence or absence of RNase H or RNase A (New England Biolabs) at a low salt concentration followed by Protease K digestion (as above) ...
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bioRxiv - Cell Biology 2023Quote: ... Double-stranded cDNA was generated by second-strand synthesis via the nick translation method using a mix containing 2Lμl of RNAse H (NEB, #M0297S), 1Lμl of E ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37 °C for 1 h and ligated to a 3′ adaptor sequence using T4 RNA ligase 2 (truncated K227Q, New England Biolabs) at 22 °C for 3 h ...
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bioRxiv - Microbiology 2023Quote: Lysates of U2OS cells infected with wild-type HSV-2 186 at an MOI of 3 for 24 h were treated with calf intestinal alkaline phosphatase (CIP) (New England BioLabs) as described previously60.
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bioRxiv - Cell Biology 2023Quote: ... Two 100 μl aliquots were incubated overnight at 37°C with and without 1 U Endo H (NEB, Frankfurt, Germany). Then the samples were heated with 100 μl SDS sample buffer for 5 min at 95°C.
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bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The unedited methylated DNA template present in the remaining sample was digested for 1 h at 37°C with DpnI restriction enzyme (New England Biolabs) in CutSmart® Buffer (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... followed by digestion to ribonucleotides by incubation for 2 h at 45 °C with 0.15 U of Nuclease P1 (NEB M0660S) in 10 mM ammonium acetate pH 5 ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The slides were washed three times with 1× PBS and incubated with or without 60 U/mL RNase H (M0297S, NEB) at 37°C for 36 h or left untreated ...
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bioRxiv - Genomics 2024Quote: ... RNA was the digested by adding 0.5 µL each of RNAse A and RNAse H (ThermoFisher R1253 and NEB M0297L) to each reaction and incubating for 30 min at 37 °C ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 20 pmol of the dephosporylated RNA were 5’-end-labeled (20 µCi of 32P-γATP) in a 20 µL reaction for 1 h at 37°C using 1 U polynucleotide kinase (NEB). Labeled RNA was purified on a G50-column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated with 100 μl CutSmart buffer containing 5 mM DTT and 1 mM dATP for 1 hour at room temperature before incubation for 2 h at 37°C in another 100 μl of the same buffer containing 1 μl Klenow exo-(NEB M0212S) and 1 μl T4 PNK (NEB M0201S) ...
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bioRxiv - Biochemistry 2020Quote: ... The dried glycoproteins were incubated with trypsin for 16 h at 37°C before releasing N-glycans using PNGase F (New England Biolabs, MA). Liberated N-glycans were separated from glycopeptides by C18 Sep Pak SPE cartridges (Waters Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... 1.5 mg of pNL003 plasmid (38) was linearized by incubation at 37 °C for 2-4 h in the presence of EcoRV (NEB R0195S). Transcription reactions (10 mL ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 µl Hind III (Fisher, FD0504), 3 µl EcoRI (Fisher, FD0274), and 3 µl Bam HI (Fisher, FD0054) in RNase H buffer (NEB, M0297) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 µg DNA was digested with 3 µl Hind III (Fisher, FD0504), 3 µl EcoRI (Fisher, FD0274), and 3 µl Bam HI (Fisher, FD0054) +/-5 µl RNase H (NEB, M0297) in RNase H buffer (NEB ...
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bioRxiv - Microbiology 2021Quote: A549 cells were infected with PR8 at a MOI of 5 and lysates were collected every two hours post infection (hpi) until 8 h RNA extraction was performed with Monarch total RNA Miniprep kit (New England Biolabs GmbH) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of the extracted genomic DNA was digested for 4 h at 37☐ using 10 U MmeI (New England Biolabs). The DNA was then immediately dephosphorylated by treatment with 1 U calf intestine alkaline phosphatase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL of the IEC fraction was concentrated for 4 h in vacuum to which 20 μL of the dried sample and 2 μL of 10xGlyco Buffer (New England Biolabs™) were added into 2 mL Eppendorf tube and incubated in the thermomixer at 99 °C for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µL of a 1000 U of DNase I stock was added to 500 µL of DNase I buffer on cells for 10 minutes or 2 µL of a 5000 U of RNase H stock (NEB M0297S) was added to 500 µL of RNase H buffer on cells for 5 minutes at 45 °C ...
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bioRxiv - Genomics 2021Quote: ... The sample was then allowed to cool to room temperature and incorporated in the Klenow enzyme mix (1x Maxima h-RT buffer, 1mM dNTP, 1U/μL of Klenow Exo-; NEB M0212L) was added to the single strand library ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Purified PCR products and the destination vector pSIN-TRE3G-GFP-miRE-PGK-Neo were digested with XhoI/EcoRI 37°C for 1 h and the PCR product was ligated into the destination vector using T4 DNA ligase (NEB, # M0202L) overnight at 16 °C.
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bioRxiv - Biophysics 2023Quote: ... These plasmids were digested with NotI-HF and XhoI for 2 h at 37°C (R3189, R0146, New England Biolabs, UK) and heat-inactivated for 20 min at 80°C.
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bioRxiv - Cancer Biology 2023Quote: ... The spheres were collected after 24 h and RNA extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs #T2010S). RNA was extracted from adherent cells directly from the plate ...
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bioRxiv - Genetics 2023Quote: ... After 48 h cells were incubated for 30 min at 37 °C with SNAP-Oregon green (NEB, final concentration 4 µM). Cells were then incubated for 30 min at 37 °C in fresh media to wash off unbound substrate ...
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bioRxiv - Cell Biology 2022Quote: ... 6 μg of digested nucleic acids were treated overnight at 37 °C with or without 10 μl of RNase H (New England BioLabs, M029L) in 1x RNase H buffer and 1/10th of the samples was saved as input ...
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bioRxiv - Microbiology 2023Quote: ... or treated with 0.1 mM carbonic anhydrase inhibitor – S4 for 24 h was subjected to semi-quantitative RT-PCR analyses using Q5 high-fidelity DNA polymerase (New England Biolabs Inc.) according to manufacturer’s protocol in a T100 Thermal cycler (BIO-RAD) ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin handle and cosmid-I95 DNA were both digested for 2 h at 37 °C with SpeI-HF (New England Biolabs, R3133L) and subsequently heat-inactivated for 20 min at 80 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-myc (New England Biolabs, clone 9B11, 1:2,000), rabbit α-pol-I largest subunit 15 (1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... mouse monoclonal antibodies against PAR (clone 10H, Tulip BioLabs, USA), anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... and mid-range PFG ladder (for mouse samples) (NEB Inc.) and 1 kb ladder (GeneDirex Inc.) ...
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bioRxiv - Genomics 2019Quote: ... for 1 h at 37 C followed by incubation with 80 U of TaqDNA ligase (New England Biolabs; 50 C/ 30 min). DNA was extracted ...
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bioRxiv - Microbiology 2021Quote: Vector pFLD was digested with PmlI at 37°C for 2 h and dephosphorylated with 5 U Antarctic phosphatase at 37 °C for 30 min as recommended by the manufacturer (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of TET21-N cells (Control and 24 h doxycycline) was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, Hitchin, UK), from which complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 16 °C for 4 h in the presence of 1.15x of T4 ligation buffer (New England Biolabs, catalog no. B0202S) and 100U T4 ligase ...
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bioRxiv - Microbiology 2023Quote: ... DNA was ligated for 4 h in a water bath at 16 °C (30 Weiss Units of T4 DNA Ligase, M0202 New England BioLabs® Inc). 300 μg of Proteinase K was used to reverse the cross-linking overnight at 65°C ...
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bioRxiv - Genetics 2024Quote: ... A total of 25 µg DNA was either mock digested or digested with 30 units of RNAse H (New England Biolabs, Ipswich, MA) in 100 µl final volume at 37 °C for 6 hours ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified from mouse cDNA by polymerase chain reaction (NEB, M0492L) using primers below:
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bioRxiv - Immunology 2021Quote: ... total RNA was isolated from uninfected and FV3-infected Xela DS2 cells at 24 h post-infection (hpi) and 72 hpi using the Monarch Total RNA Minipreps kit (New England Biolabs, Ipswich, United States) and genomic DNA was removed using on-column DNase I digestion ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were dried in a speed vac before peptides were deglycosylated with Endo H or PNGase F according to manufacturer’s instructions (P07025 & P0710S, New England Biolabs Inc., Hitchin, United Kingdom).