Labshake search
Citations for New England Biolabs :
501 - 550 of 1221 citations for Mouse Anti Crimean Congo Hemorrhagic Fever Virus Gn Protein JE12 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... The protein sample was concentrated by ultrafiltration and incubated with PNGaseF (New England Biolabs) overnight.
-
bioRxiv - Neuroscience 2022Quote: Recombinant proteins were labeled with SNAP-Surface 549 dye (New England BioLabs, Cat# S9112S) for visualization ...
-
bioRxiv - Biophysics 2022Quote: ... purified proteins were labelled with SNAP-Surface Alexa Fluor 647 tag (New England Biolabs) following manufacturer’s instructions.
-
bioRxiv - Biochemistry 2022Quote: ... The denatured proteins were then supplemented with 500 U of EndoH-MBP (EndoHf, NEB) and incubated for 45 min at 37°C ...
-
bioRxiv - Biochemistry 2022Quote: ... All other proteins were expressed in Escherichia coli BL21(DE3) cells (New England BioLabs).
-
bioRxiv - Cell Biology 2019Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein was degraded by adding 50 µl of 20mg/ml proteinase K (NEB, P8102), 50 µl of 10% SDS and incubated at 55°C for 30 minutes ...
-
bioRxiv - Immunology 2020Quote: For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at RT for 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-bound proteins were eluted by adding 7.5 μl RNase H (NEB), 2 μl TURBO DNase (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2021Quote: Recombinant proteins from Escherichia coli lysates were immobilized on amylose resins (New England Biolabs), incubated for 1 h at 4 °C with purified GST-SH3P2 ...
-
bioRxiv - Cell Biology 2019Quote: ... one was treated with 400U Lambda protein phosphatase (Lambda PP, New England Biolabs, #P0753S) in 50µl phosphatase assay buffer ...
-
bioRxiv - Immunology 2020Quote: ... For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at room temperature for 10 min ...
-
bioRxiv - Genomics 2021Quote: ... Proteins were then mixed with Buffer A supplemented with S-adenosyl-methionine (NEB B9003S) containing 1 ug of either naked unmethylated DNA (Plasmid ASP3552 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and biotin-labelled proteins were isolated by immunoprecipitation using streptavidin magnetic beads (NEB, S1420S) in an immunoprecipitation buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2022Quote: ... aliquots of S proteins were digested respectively using alpha lytic protease (New England BioLabs), chymotrypsin (Athens Research and Technology) ...
-
bioRxiv - Plant Biology 2022Quote: ... For recombinant protein expression we used GW versions of pMAL-C2 (New England Biolabs) and pDEST-17 (Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: ... and protein purification was carried out according to the manual of Amylose Resin (NEB). The protein ...
-
bioRxiv - Microbiology 2022Quote: ... The final injection mix contained 300 ng/μL spCas9 protein (M0386, New England Biolabs), 40 ng/μL of each sgRNA (3 targeting exon 5 and 7 of Dicer2 and 4 targeting the kmo gene ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein amounts for deadenylation assays were estimated against BSA standards (New England Biolabs, B9000S).
-
bioRxiv - Molecular Biology 2022Quote: ... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2023Quote: ... The purified protein was labeled with SNAP-Surface Alexa Fluor647 dye (New England Biolabs). The fractional dye labeling and DNA binding activity were assessed as previously described [12].
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (NewEngland Biolabs) as a standard ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were then degraded by addition of 1 μL of Proteinase K (NEB #P8107) and incubation at 56°C for 30 min ...
-
bioRxiv - Immunology 2023Quote: For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at room temperature for 10 minutes ...
-
bioRxiv - Physiology 2023Quote: ... for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S, NEB) for 16h at 30°C.
-
bioRxiv - Physiology 2023Quote: ... The proteins were then purified by affinity chromatography using amylose resin (Catalog E8021L, NEB). The amylose resin was washed and pre-equilibrated in 20 mM tris ...
-
bioRxiv - Cell Biology 2023Quote: ... MBP-tagged CCDC22 and CCDC93 proteins were purified using Amylose beads (New England Biolabs), mixed in approximately 1:1 stoichiometry ...
-
bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were purified on amylose resin (New England Biolabs, Ipswich, MA, USA; Cat# E8021) and eluted with maltose using the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 µl of a broad range color pre-stained protein standard (New England BioLabs) was added to the outer lanes ...
-
bioRxiv - Cell Biology 2023Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
-
bioRxiv - Cell Biology 2023Quote: ... 100 µg of lysates were deglycosylated using Protein Deglycosylation Mix II (NEB Cat# P6044S). In short ...
-
bioRxiv - Immunology 2023Quote: ... with a mixture of 0.33 µM EnGen Cas9 protein (New England Biolabs, Cat.No. M0646T), two Cas9 guides RNA ...
-
bioRxiv - Biochemistry 2023Quote: ... Assays were set-up using PURExpress in vitro protein synthesis kit (New England Biolabs) with a cloned full-length DHFR template ...
-
bioRxiv - Cell Biology 2023Quote: ... cleaved protein product was passed through Ni-NTA resin and amylose resin (E8021, NEB) to remove the TEV protease and HIS-MBP tag ...
-
bioRxiv - Biochemistry 2024Quote: ... Bound proteins were eluted by digestion with 500 units micrococcal nuclease (New England Biolabs) for 10 minutes at 37°C in 25 mM HEPES-KOH (pH 7.6) ...
-
bioRxiv - Biochemistry 2024Quote: ... The proteins were purified on the amylose resin (New England Biolabs, Ipswich, MA, USA) and stored at −20 °C in 25 % glycerol ...
-
bioRxiv - Neuroscience 2021Quote: ... Plasmids encoding mouse Nav1.2 (NP_001092768.1) and Nav1.6 (NP_001070967.1) were cloned by Gibson Assembly (NEB) with synthetic gBlocks gene fragments (Integrated DNA Technologies) ...
-
bioRxiv - Cell Biology 2022Quote: Mouse phogrin was obtained from the IMAGE consortium (clone BC_133678) and CLIPf from NEB. The fusion protein was generated by standard molecular cloning techniques ...
-
bioRxiv - Neuroscience 2020Quote: ... Mouse Adnp was amplified from pENTR223.1-Adnp using Q5 High Fidelity DNA Polymerase (NEB) and primers containing 6xHis tag to insert it into the C-terminal region of Adnp ...
-
bioRxiv - Microbiology 2020Quote: Fetuin was deglycosylated under denaturing conditions using Protein Deglycosylation Mix II kit (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... GST fusion protein on glutathione beads was incubated with 250 units CK2 (New England Biolabs) in protein kinase buffer (New England Biolabs ...
-
bioRxiv - Biochemistry 2022Quote: ... Association occurred in samples containing ACE2- fusion proteins either without or with TEV protease (NEB) treatment ...
-
bioRxiv - Molecular Biology 2020Quote: ... and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs, #M0251S) by 10% SDS-PAGE gel electrophoresis stained with Coomassie blue (Bio-rad).
-
bioRxiv - Molecular Biology 2021Quote: The hybrid protein and AβBP were cloned into pMAL-C5E MBP vector (New England Biolabs) with a cleavage site for 3C protease immediately after MBP ...
-
bioRxiv - Microbiology 2020Quote: ... The sgRNAs were then associated with EnGen® Spy Cas9 NLS protein (New England BioLabs) at room temperature for 20 min ...
-
bioRxiv - Cell Biology 2019Quote: ... The bead-bound protein was incubated with 3 µM SNAP-Surface Alexa Fluor 647 (NEB) for 40-60 min at 4°C (to fluorescently label the protein) ...
-
bioRxiv - Biophysics 2019Quote: ... using a 5-fold excess of fluorophore to protein and ∼1 μM Sfp synthase (NEB) for 16 hours at 6 °C in 50 mM TRIS-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... Eluted proteins were incubated with TEV protease and MBP-fusion endoglycosidase (EndoHf, New England Biolabs) at 4 °C overnight ...
-
bioRxiv - Genomics 2021Quote: - T4 gene 32 protein (10 mg/mL, NEB or EPFL “homemade” T4g32p, 10 mg/mL). In 96-well plates ...