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Citations for New England Biolabs :
501 - 550 of 2293 citations for Mouse ANKRA2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The digested plasmid was then blunted using Quick Blunting kit (NEB) and re-ligated using T4 DNA Ligase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were linearized with the AscI enzyme (New England Biolabs, #R0558S) for SINV and NotI enzyme (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... The exon 1 targeting plasmid was digested with XhoI/KpnI (NEB), and the exon 7 guide was amplified with primers 22 and 23 to facilitate gibson cloning of the exon 7 guide into the same backbone ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid pool was linearised overnight with KpnI-HF (NEB, R3142), purified by phenol-chloroform precipitation then resuspended in P3 (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were constructed using Gibson Assembly (New England Biolabs, Ipswich, MA). Regions of the plasmids constructed using PCR were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Inserts and plasmids were digested with restriction enzymes (New England BioLabs), gel purified (GeneJet Gel Extraction Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmid DNA (2 µg) was digested with BsmBI-v2 (NEB, R0739) at 55°C for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids with the correct sequence were linearized with ScaI-HF (NEB) and transformed into the B ...
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bioRxiv - Molecular Biology 2023Quote: ... Transgenesis plasmids were generated using NEBuilder HiFi Assembly Master Mix (NEB) and primers listed in Supplementary Table 2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the plasmid was constructed using NEBuilder HiFi DNA assembly (NEB). All oligos used are available in the table below.
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... Sanger sequencing was performed to validate plasmid assembly and SmaI (NEB) digestion to monitor the integrity of the ITRs ...
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bioRxiv - Cancer Biology 2023Quote: ... The pX330+Cre plasmid was digested with BbsI (New England BioLabs) and ligated to annealed and phosphorylated sgRNA oligonucleotides (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µg of pUPRT plasmid was linearised with AgeI-HF (NEB) (GRA59 ...
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bioRxiv - Developmental Biology 2023Quote: ... A repair plasmid was made using NE Builder (New England Biolabs) that includes a 430bp gypsy insulator sequence (Geyer and Corces 1992 ...
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bioRxiv - Biochemistry 2023Quote: ... Backbone plasmids were digested with relevant restriction enzymes (New England Biolabs). Desired DNA fragments to be inserted were amplified by PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... by digesting the plasmid with StuI and NheI (New England Biolabs) using gBlocks synthetic DNA fragments (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid ISceI/MCS-d1GFP-SV40/ISceI was digested with XhoI (NEB) and SmaI (NEB) ...
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bioRxiv - Immunology 2023Quote: ... Mutations were introduced in the synthesized plasmids by Q5 mutagenesis (NEB).
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bioRxiv - Cell Biology 2023Quote: ... Parental plasmid was digested by restriction enzyme DpnI (New England Biolabs) and linearized plasmid was isolated by gel purification (Zymo Research) ...
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bioRxiv - Neuroscience 2023Quote: Plasmid constructs were linearized using XbaI restriction enzyme (New England Biolabs) to create DNA templates for complementary RNA (cRNA ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids were constructed using a NEBuilder HiFi DNA Assembly kit (NEB) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... the plasmids were linearized by digestion with SpeI (New England BioLabs), followed by purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of plasmid was incubated with 8 U M.SssI (NEB) at 37°C for 4 h for in vitro DNA methylation ...
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bioRxiv - Genomics 2024Quote: ... Tet-puro-pLKO plasmid was digested with AgeI and EcoRI (NEB) restriction enzymes ...
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bioRxiv - Microbiology 2024Quote: ... plasmid was double digested with NdeI and SpeI restriction enzymes (NEB) followed by gel purification of the 4725 bp band using a Wizard spin column (Promega) ...
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bioRxiv - Systems Biology 2024Quote: Each tagged-ORF plasmid subpool was treated with I-SceI (NEB) in the provided buffer (at a volume of 50uL for ∼1ug hORF plasmids and 200uL for ∼4ug vORF plasmids) ...
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bioRxiv - Biophysics 2023Quote: ... The LRRC8A-GFP-PhoCl-TMEM plasmid was digested with AgeI (NEB) and EcoRI (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB). The identity of all plasmids was confirmed by Sanger Sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... To clone inserts into plasmids NEBuilder® HiFi DNA Assembly (NEB) was used.
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bioRxiv - Microbiology 2024Quote: ... Plasmids and inserts were assembled using NEBuilder HiFi DNA Assembly (NEB), followed by incubation at 50°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using isothermal HiFi assembly (New England Biolabs (NEB), Ipswitch ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using isothermal HiFi assembly (New England Biolabs (NEB), Ipswitch ...
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bioRxiv - Developmental Biology 2024Quote: ... pXPR_050 plasmids were digested using the Bsmb1 restriction enzyme (NEB; R0580). The synthesized double-strand oligo pools and digested pXPR_050 plasmids were ligated using T7 ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were constructed using T4 DNA ligase (New England Biolabs) with a ratio of 3:1 of insert to backbone ...
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bioRxiv - Microbiology 2024Quote: ... linear plasmid backbone was generated by restriction digestion with SmaI (NEB). Plasmid backbone DNA was treated with DpnI (NEB ...
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bioRxiv - Cell Biology 2024Quote: Plasmid cloning was carried out using NEBuilder HIFI DNA assembly (NEB). Primer sequences are given in Table 1 ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was linearized using BAMHI-HF (New England Biolabs, USA) following manufacturer protocol ...
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bioRxiv - Bioengineering 2024Quote: The glr-1p::GCaMP6s plasmid was made by LR recombination (NEB) of a destination vector containing the DNA sequences for GCaMP6s (Chen et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... MBP (mouse, 1:30,000, New England Biolabs), FLAG (mouse ...
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bioRxiv - Plant Biology 2020Quote: ... Unmodified plasmids were digested with methylation-sensitive enzyme DpnI (New England Biolabs). The mutated plasmids were transformed in E ...
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bioRxiv - Biophysics 2021Quote: ... and cloned into BsaI-restricted PGEM 601 plasmids using T4 ligase (NEB). Ligation product was transformed into E ...
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bioRxiv - Biophysics 2021Quote: ... and cloned into KpnI-BamHI-digested pFL38 plasmid by T4 ligase (NEB). The AAC2 null strain was transformed with pFL38-AAC2-flag ...
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bioRxiv - Molecular Biology 2019Quote: ... Unmethylated control plasmid was produced in dam− bacteria (New England BioLabs #C2925H) and methylation status was confirmed by DpnI and DpnII digestion ...
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bioRxiv - Genetics 2022Quote: ... The linearised plasmid was blunted using T4 DNA polymerase (New England Biolabs) and re-ligated.
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bioRxiv - Neuroscience 2021Quote: ... pX330 vector (addgene plasmid #42230) was digested with BbsI (NEW ENGLAND BIOLABS), and each sgRNA sequence was cloned into the sgRNA cloning site as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified plasmids were linearized by overnight digestion (37°) with EcoRI-HF (NEB) and the products were then visualized on 1% agarose gel ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid constructs were modified by site-directed mutagenesis (E0554; New England Biolabs) to create the desired mutations in klp-6 and cwp-4 ...
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bioRxiv - Molecular Biology 2021Quote: PHO4 was cloned into the 2micron plasmid pRS424 using Gibson assembly (NEB). pGAL-H3-3xHA-URA3 on a single copy plasmid is a gift from the Michel Strubin lab.