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Citations for New England Biolabs :
51 - 100 of 250 citations for Measles virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysates were incubated with H4K5 Bu antibody (PTM Biolabs) or preimmune IgG per sample and 25 μl of magnetic protein G Dynabeads (catalog no ...
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bioRxiv - Biochemistry 2021Quote: Whole cell lysate or 5 μL of PURExpress (New England BioLabs) reaction was loaded onto a nitrocellulose membrane (Pall Corporation ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysate was added to 10 mL Chitin Resin (NEB S6651S) pre-equilibrated with Tn5 Lysis/Wash buffer and rocked for 1 hour at 4°C ...
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bioRxiv - Immunology 2022Quote: ... lysates were treated with PNGase F or EndoH (New England Biolabs) following manufacturer’s recommendation.
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bioRxiv - Microbiology 2022Quote: ... The lysates were sonicated and denatured with Glycoprotein Denaturing Buffer (NEB) by heating them at 100°C for 10 minutes ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified before loading onto the chitin resin (NEB). The sample was treated with 3 U/μL of micrococcal nuclease (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were first incubated with Deglycosylation Mix Buffer 2 (NEB) at 75°C for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 16.5 µl cleared lysate and uracil DNA glycosylase (UDG; NEB, 1.25 units). Abasic site cleavage was induced by the addition of 100 mM NaOH and incubation at 95° C ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was passed over chitin resin (New England Biolabs, MA) by gravity ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation and then applied to amylose resin (NEB). Resin was washed with 10x column volumes of Column Buffer ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were treated with endoglycosidase H (Endo H) (New England BioLabs) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were mixed with 30 μl of SNAP-magnetic beads (NEB) and incubated for 1 h at 24°C on a thermomixer (900 rpm) ...
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bioRxiv - Cell Biology 2021Quote: ... clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Biochemistry 2020Quote: ... 75 mM NaCl) from clarified lysates using Chitin Resin (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... The cleared cell lysate was applied to Amylose resin (New England Biolabs) followed by washing with buffer A and elution with buffer A containing 10 mM maltose ...
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bioRxiv - Cell Biology 2020Quote: ... lysate nanobody mixture was incubated with amylose resin (NEB Cat No. E8021L) for 2 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The lysate and the precipitates were treated with Lambda Protein Phosphatase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... CaMKAR was isolated from soluble lysate by nickel chromatography NEBExpress columns (NEB). Soluble protein was quantified by Pierce BCA assay (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 h cell lysates were treated with PNGase-F (NEB, P0704L) or water by following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 75 mM NaCl) from clarified lysates using Chitin Resin (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... The lysates were clarified and loaded on 1 mL chitin resin (NEB) and washed with 1 M KCl ...
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bioRxiv - Cell Biology 2023Quote: ... the lysate was loaded into the cobalt affinity column (New England Biolabs) and eluted with a buffer containing 30 mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared cell lysate was applied to Amylose resin (New England Biolabs), followed by washing with buffer A and elution with buffer A containing 10 mM maltose ...
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bioRxiv - Microbiology 2021Quote: ... in the 5’end of 30 mer RNA substrate we used the vaccinia virus capping enzyme following the manufacturer’s protocol (New England Biolabs), except that the methyl donor SAM was absent and 0.05 units of inorganic pyrophosphatase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2 -O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2’-O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Microbiology 2024Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2L-O-methyltransferase (New England Biolabs). The mRNA was purified ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids encoding HA-tagged LC3B proteins were generated by replacing the EGFP sequence in the EGFP plasmids with an HA-tag followed by a Tobacco etch virus protease sequence recognition site and a Flag-Tag (Genewiz) using AgeI and HindIII (New England Biolabs). Lipidation-deficient LC3B proteins were generated by mutagenic PCR using Q5 Hot Start High-Fidelity Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA with retrotranscriptase from Moloney murine leukemia virus (Ref. M0253, New England BioLabs) and random hexanucleotides as primers by following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The cap 0 standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with Vaccinia virus capping enzyme (VCE, NEB) in the presence of GTP and S-adenosylmethionine (SAM) ...
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bioRxiv - Biochemistry 2020Quote: ... a maltose binding protein (MBP) and a Tobacco Etch Virus (TEV) protease cleavage site in the N-terminal via Gibson assembly (New England Biolabs) as per the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2021Quote: ... The DNA depleted RNA was used as the template for reverse transcription performed with Moloney murine leukemia virus (MMLV) reverse transcriptase (M0253, NEB). The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with genespecific primers using SsoAdvanced Universal Sybr green Supermix (1725271 ...
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bioRxiv - Microbiology 2021Quote: ... Virus titer was determined using SYBR-based one step qRT-PCR with Luna Universal One Step qRT-PCR reagent (NEB). QuantStudio 5 (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... The DNA depleted RNA was used as the template for reverse transcription performed with Moloney murine leukemia virus (MMLV) reverse transcriptase (M0253, NEB). The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with gene-specific primers using SsoAdvanced Universal Sybr green Supermix (1725271 ...
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bioRxiv - Developmental Biology 2022Quote: ... using 200-300 ng of purified RNA as template and Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase (M0253, NEB). cDNA was synthesised using the standard first strand synthesis protocol with random hexamers (S1230 ...
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bioRxiv - Plant Biology 2022Quote: All ObANS genes were inserted into the pBI121 plasmid under the control of the constitutive Cauliflower mosaic virus 35S promoter using the SacI and XbaI restriction enzymes (Thermo-scientific) and the NEBuilder ligation system (NEB). The plasmids were transformed into Agrobacterium tumefaciens strain GV3101 using electrotransformation and positive colonies were selected with kanamycin ...
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bioRxiv - Molecular Biology 2023Quote: ... A CRISPR array containing two repeats and one spacer targeting the gfp gene of recombinant Sindbis virus (SINV-GFP) was generated by annealing and extending two partially complementary DNA oligos with Q5 polymerase (NEB) (Table S1) ...
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bioRxiv - Physiology 2023Quote: ... was obtained by reverse transcription (RT) using 1 μg of RNA and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (M0253, NEB), using the first strand cDNA synthesis standard protocol with random primers (S1330 ...
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bioRxiv - Microbiology 2024Quote: The ectodomains of the hemagglutinin proteins from selected influenza virus strains were ordered as synthetic DNA fragments from Twist Biosciences and cloned with a barcoded fragment encoding the last 46 amino acids of WSN HA as 3-segment assembly reaction into a construct containing the 3’ non-coding region of the packaging signal and signal peptide from A/WSN/1933 influenza HA and the a Read 1 Illumina sequence and the full 5’ packaging signal from A/WSN/1933 virus using Hifi Assembly Mastermix (NEB). The backbone for this cloning reaction was a pHH21 plasmid (16 ...
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bioRxiv - Microbiology 2024Quote: ... hepatitis delta virus (HDV) ribozyme and simian virus 40 (SV40) poly-A sequence using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) at 50 °C for 60 min ...
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bioRxiv - Biochemistry 2023Quote: ... modified to include a tobacco etch virus (TEV) protease recognition sequence downstream of the 6xHis-tag47 using NEBuilder HiFi DNA assembly master mix (New England BioLabs). Sequences were verified by Sanger DNA sequencing [Genomics Shared Resource ...
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bioRxiv - Neuroscience 2021Quote: ... Glycosylation was removed from denatured extracted lysates using PNGase F (NEB, Cat# P0704S) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Phosphatase treatment was carried out on boiled lysates using alkaline phosphatase (NEB #M0290) for at least one hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 50 µg cell lysate was treated with 80 units of lambda-phosphatase (NEB) at 30°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... and lysate was incubated 37°C 60 min in 2 units CIP (NEB) per 50 μL reaction containing 50μg of total protein ...
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bioRxiv - Cell Biology 2022Quote: ... 20µL of this lysate was mixed with 800U lambda phosphatase (New England Biolabs) or untreated ...
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bioRxiv - Molecular Biology 2020Quote: ... Clear lysate fraction was added to 1.5 ml magnetic chitin beads (NEB E8036S) equilibrated in lysis buffer ...