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Citations for New England Biolabs :
401 - 450 of 3821 citations for MEK Kinase Kinase 1 MAP4K1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... After end repair (T4 DNA polymerase, T4 DNA polynucleotide kinase, Klenow (all NEB) in the presence of dNTPs in ligation buffer (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Microbiology 2022Quote: ... the RNA 5′ ends were phosphorylated with T4 polynucleotide kinase (New England Biolabs). Adapters were ligated to the 5′ and 3′ ends of the RNA and first-strand cDNA synthesis was performed using M-MLV reverse transcriptase ...
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bioRxiv - Microbiology 2022Quote: ... Linear products were phosphorylated with T4 Polynucleotide Kinase (New England Biolabs, MA, USA) for 1 hr at 37°C and circularized with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... the linear PCR product was phosphorylated using T4 polynucleotide kinase (New England Biolabs) and ligated with T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The annealed oligos were phosphorylated using T4 polynucleotide kinase (PNK, New England Biolabs). The phosphorylated and annealed oligos were then diluted in Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2022Quote: ... and 10 units T4 Polynucleotide Kinase (PNK) enzyme (New England Biolabs, cat#M0201S) for 2 hours at 37°C in 1X T4 PNK buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.75 µl 0.1 M DTT and 0.3 U/µl T4 Polynucleotide kinase (NEB), and then incubated at 37°C 30 min ...
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bioRxiv - Genetics 2023Quote: ... and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB). DNA was circularized at a concentration of 5 ng/μL with T4 DNA ligase (NEB) ...
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bioRxiv - Immunology 2023Quote: ... RNA fragments were then 3lll-dephosphorylated using T4 polynucleotide kinase (New England Biolabs) for 6 hours at 37°C in 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was phosphorylated for 1h at 37°C with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Genetics 2023Quote: ... The ICU11_sgRNA1_F/R oligonucleotides were phosphorylated using T4 polynucleotide kinase (New England Biolabs) and hybridized in a thermal cycler (Bio-Rad Laboratories T100) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Hairpin oligonucleotides were phosphorylated by T4 polynucleotide kinase (New England Biolabs, Beverly, MA) followed by annealing at 100°C for 5min and cooling in the heat block for 3h ...
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bioRxiv - Biochemistry 2022Quote: ... were run and then phosphorylated and re-ligated using T4 polynucleotide kinase (NEB) and T4 ligase (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... 4 U/mL creatine kinase) containing 10 µMnt cssDNA (NEB, PhiX virion DNA) was supplemented with Rad51 (5 µM ...
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bioRxiv - Microbiology 2023Quote: ... 6 ng of sample was treated with 2 units T4 Polynucleotide Kinase (NEB) and incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was phosphorylated at the 5’ end by T4 polynucleotide kinase (NEB) and ligated to the 5’ adapter (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ ends were hydroxy-repaired by adding T4 polynucleotide kinase reaction mix (NEB) which was incubated for 45 minutes at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Single strand DNA oligonucleotides were ligated using T4 Polynucleuotide Kinase (New England Biolabs) using manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by end repair with T4 Polynucleotide Kinase (T4 PNK; NEB, Cat# M0201) at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: Ribosome-protected fragments were dephosphorylated using T4 Polynucleotide Kinase (M0201, New England Biolabs). Dephosphorylated RNA was extracted using ROTI Acqua P/C/I for RNA extraction (X985.1 ...
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bioRxiv - Genomics 2024Quote: ... and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB). DNA was circularized at a concentration of 5 ng/µl with T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM SARS_139 and SARS_140 primers were mixed with 1 μl T4 Polynucleotide kinase (PNK; NEB) in 1x PNK reaction buffer (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA probes (Extended Table 1) at 500 nM were 5’-[32P]-labeled using T4 Polynucleotide Kinase (NEB) and hybridized to the membrane overnight at 37°C in PerfectHyb™ Plus Hybridization Buffer (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 20 pmol of the obtained RNA were then radiolabeled by incubation with 1 U of Polynucleotide Kinase (NEB) and 20 μCi of 32P-γATP for 1 h at 37°C in a 20 μL reaction volume ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... top and bottom oligonucleotides for a single R-S-R or dead end oligonucleotide pairs were combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using T4 Polynucleotide Kinase (NEB M0201L) for 2 h at 37 °C ...
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bioRxiv - Biophysics 2019Quote: ... the 5’ ends of the PCR products were phosphorylated by T4 polynucleotide kinase (NEB), the products were purified and the free ends of the plasmid re-ligated by T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2021Quote: The RNA was then fragmented in 0.25X T4 Polynucleotide kinase (PNK) buffer (NEB M0201) (1 µl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The purified RNA was then treated with 20 units of T4 polynucleotide kinase (NEB) for 3h at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Oligonucleotides were radio-labeled with [γ-32P]ATP with the T4 polynucleotide kinase (NEB).
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bioRxiv - Microbiology 2021Quote: ... Fragments were then end-labeled with γ-32P-ATP using T4 polynucleotide kinase (NEB), separated on 15% TBE-Urea gels and visualized by autoradiography ...
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bioRxiv - Microbiology 2021Quote: ... The samples were dephosphorylated by incubating with 10 U of T4 Polynucleotide Kinase (NEB) for 1 hour at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Complimentary sgRNA oligos were phosphorylated with T4 polynucleotide kinase (New England Biolabs cat # M0201S) according to manufactures protocol and annealed to each other using a Bio-Rad C1000 thermocycler with the following reaction conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... act57B sequence: 5’-UCUUCCCCUC-3’ RNA oligonucleotides were end-labeled using T4 Kinase (NEB) with ATP [γ-32P]32 ...
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bioRxiv - Molecular Biology 2020Quote: ... The nucleic acids were subsequently radiolabeled with γ32P-ATP using T4 polynucleotide kinase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The 4mt-GCamP6f PCR product was phosphorylated using T4 Polynucleotide Kinase (New England Biolabs) and digested using AscI ...
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bioRxiv - Plant Biology 2022Quote: ... Probes were end-labelled using [γ-32P]ATP and polynucleotide kinase (New England Biolabs), according to (Sambrook et al ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by labeling with γ-P32-ATP and T4 polynucleotide kinase (Cat#: M0201L, NEB). The reactions were further processed and digested with P1 Nuclease (Cat# ...
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bioRxiv - Molecular Biology 2023Quote: ... T4- PNK (polynucleotide kinase) and NheI enzymes were purchased from New England Biolab (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... Size selected RNA was dephosphorylated by T4 polynucleotide kinase (PNK, New England Biolabs, B0201S) treatment for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cost of Phospho-RNA-seq was estimated using T4 polynucleotide kinase (NEB, M0201S) and TruSeq small RNA kit (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
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bioRxiv - Cancer Biology 2022Quote: ... 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK) (NEB, M0201). The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the fragmented DNA was blunt-end repaired with T4 Polynucleotide Kinase (New England Biolabs) and then purified with the MinElute Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... cDNA was end repair and A tailed with T4 polynucleotide kinase (New England Biolabs). The sequencing library was prepared using SMRTbell Express Template Prep Kit 2.0 following the standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA fragments were dephosphorylated by incubation with T4 polynucleotide kinase (New England Biolabs) for 1 h at 37°C and ligated to preadenylated linker oligonucleotides (5′-App NNNNNIIIIIAGATCGGAAGAGCACACGTCTGAA-ddC-3′ ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
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bioRxiv - Molecular Biology 2023Quote: ... and then 3’-end dephosphorylated and 5’-end phosphorylated using T4 polynucleotide kinase (NEB). tRNA library preparation used SuperScript™ IV following the QuantM-tRNA-seq protocol (Pinkard et al ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were annealed and phosphorylated with 5U of T4 Polynucleotide Kinase (New England Biolabs). Methylation constructs were obtained with the Golden Gate assembly cloning strategy based on BbsI restriction enzyme ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA was ethanol precipitated and phosphorylated with T4 polynucleotide kinase (New England Biolabs) prior to library construction using the NEXTFLEX Small RNA-Seq Kit v4 (Perkin Elmer) ...