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Citations for New England Biolabs :
101 - 150 of 294 citations for M CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253, New England Biolabs) was added and the reaction was incubated at 42 °C for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2ug RNA was reverse-transcribed using M-MuLV reverse transcriptase enzyme (New England Biolabs #M0253S) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA synthesis was carried out with M-MuLV Reverse Transcriptase (New England Biolabs, #Cat no: M0253L), and qPCR reactions were conducted using gene-specific primers with SensiFAST SYBR No-ROX kit (Bioline ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM 3’-O-Me-m 7G(5’)ppp(5’)G cap structure analog (NEB S1411S), 0.5 mM GTP ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 M guanidine HCl) containing 8 units/ml and Proteinase K (P8107S, New England Biolabs Inc.) was added freshly to the digestion buffer ...
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bioRxiv - Genomics 2021Quote: DNaseI-treated RNA was reverse transcribed using M-MuLV reverse transcriptase (New England Biolabs, Ipswich, Massachusetts) and random hexamer primers ...
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bioRxiv - Developmental Biology 2022Quote: ... It was subsequently reverse transcribed into cDNA with M-MuLV reverse transcriptase (New England Biolabs M0253S). qRT-PCR was carried out using SYBR Green PCR Master Mix (Applied Biosystems 4368708 ...
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bioRxiv - Plant Biology 2022Quote: Reverse transcription was performed using 2 µg of total RNA and M-MuLV Reverse Transcriptase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA was then digested using Dpn II (375 U DpnII (NEB, R0543 T/M/L) in 1× Dpn II buffer (NEB ...
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bioRxiv - Microbiology 2023Quote: ... cDNA of the samples was made following the NEB M-MuLV Reverse Transcriptase protocol (NEB #M0253). Qubit was used to assess the concentration of cDNA in the samples ...
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bioRxiv - Biophysics 2023Quote: ... A total of 1 μg of cDNA was prepared using the M-MulV reverse transcriptase (NEB) as per vendor instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was produced from ∼1 µg of RNA using M-MuLV Reverse Transcriptase (New England Biolabs). RTqPCR was performed on 1:10 dilutions of the cDNA using iTaq Universal SYBR Green Supermix (Bio Rad ...
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bioRxiv - Immunology 2024Quote: ... cDNA synthesis was performed using ProtoScript M-MuLV First Strand cDNA synthesis kit (New England Biolabs) and random hexamer primers ...
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bioRxiv - Synthetic Biology 2019Quote: ... according to the manufacturer’s protocols (including the gDNA wipeout step) or M-MuLV reverse transcriptase (NEB, M0253L) according to manufacturer’s standard protocol (using Murine RNase Inhibitor [NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 µg RNA was used for each reverse transcriptase reaction using M-MuLV Reverse Transcriptase (NEB) according to manufacturer protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: RNA was extracted and reverse transcribed using random primers and M-MuLV reverse transcriptase (New England Biolabs) and a PCR product of about 300 nucleotides from a coding sequence was than amplified by PCR using Q5® High-Fidelity DNA polymerase (New England Biolabs) ...
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bioRxiv - Biochemistry 2020Quote: ... Approximately 200 ng/μl of RNA was used for cDNA synthesis by M-MuLV reverse transcriptase (NEB) and 3′ oligo-dT primer ...
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bioRxiv - Microbiology 2020Quote: ... The reverse transcription reaction (20 μL) contained 2 μL 10x M-MulV buffer (B0253S, New England Biolabs), 1 μL of 50 μM Oligo 18 dT (SO132 ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.25 μL of reverse transcriptase (M-MuLV Reverse Transcriptase kit from New England Biolabs, CAT# M0253S) was prepared and 3.25 μL of this master mix was added to each of the samples for a total volume of 20 μL ...
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bioRxiv - Genomics 2021Quote: ... 4 μg of total RNA were reverse-transcribed by the M-MLV reverse transcriptase (New England BioLabs) following the manufacturer specifications and using oligo d(T ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µl freshly made 1 M NH4HCO3 and calf intestinal alkaline phosphatase (1 U, New England Biolabs) were added ...
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bioRxiv - Biochemistry 2023Quote: ... M Y229A and MT205D substitutions were generated using the Quick change directed mutagenesis kit (New England Biolabs), as recommended by the manufacturer.
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from extracted RNA samples using M-MuLV reverse transcriptase kit (New England Biolabs Ltd) based on the manufacturer’s instructions and using customized primers splice leader (SL_F ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-MBP monoclonal (NEB), anti-mouse-HRP (Dianova) ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA synthesis was performed for 1 h at 42°C using M-MuLV reverse transcriptase (200 U, NEB) followed by a heat-inactivation at 80°C for 5 min ...
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bioRxiv - Genetics 2021Quote: ... 1 μg of RNA was reverse transcribed in cDNA using random hexamers and M-MuLV reverse transcriptase (NEB). qPCRs were assembled with Absolute QPCR ROX Mix (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... excess gel was removed and embedded specimen were placed in digestion buffer (1X TAE, 0.5% Triton X-100, 0.8 M guanide HCL) with 8 units/ml Proteinase K (NEB) for 2 hours at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was generated from pCG1-SARS-2-S (a kind gift from M. Hoffmann9 by PCR (Phusion polymerase (NEB)) using primer pairs ...
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bioRxiv - Developmental Biology 2019Quote: ... First Strand cDNA was synthesized using the Protoscript M-MuLV First-Strand cDNA Synthesis Kit (New England Biolabs). Equal concentrations of RNA (1ug ...
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bioRxiv - Genomics 2021Quote: ... The RNA samples were reverse transcribed using random hexamer primers and M-MuLV Reverse Transcriptase (New England Biolabs) and the resulting cDNA subjected to qPCR ...
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bioRxiv - Biophysics 2023Quote: ... 500 ng biotinylated λ DNA was incubated at 37°C with 1.6 M S-adenosylmethionine (New England BioLabs) and 20 U CpG methyltransferase M.SssI (New England BioLabs ...
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bioRxiv - Bioengineering 2023Quote: ... The RT-RPA was performed at 42°C for 30 min by combining M-MuLV-RT (NEB #M0253L) with TwistAmp Basic (TwistDx #TABAS03KIT) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg total RNA was reverse transcribed with 50 ng random hexamers using M-MuLV reverse transcriptase (NEB). 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega) ...
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bioRxiv - Genomics 2023Quote: ... The cDNAs were built by reverse transcription of proliferating MuSC mRNAs using M-MuLV Reverse Transcriptase (NEB #M0253L). mRNAs were extracted using NucleoSpin RNA Plus XS kit (Macherey-Nagel #740990.50) ...
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bioRxiv - Neuroscience 2023Quote: ... and reversely transcribed into complementary DNA (cDNA) using M-MuLV reverse transcriptase (NEB.M0253S, New Englands BioLabs, Ipswich, MA). qPCR was performed using the Brilliant II SYBR Green QPCR master mix (Agilent Technologies ...
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bioRxiv - Genetics 2023Quote: ... 1.2 M sorbitol) and resuspended in digestion buffer (Buffer B, 200 mM Vanadyl ribonucleoside complex [VRC from NEB] ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-MBP (NEB, E8032). Peroxidase and Alexa-fluor conjugated secondary antibodies were purchased from Jackson Laboratories and ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-myc (New England Biolabs), rabbit polyclonal anti- GFP (gift from Oliver Gruss ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-MBP (NEB, 1:10000); mouse α-Pol II CTD clone 8WG16 (Abcam ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cloning was done in bacterial strain Escherichia coli NEB® 10-beta (New England Biolabs, Frankfurt a. M., Germany) grown at 37 °C in lysogeny broth [47] supplemented with 60 mg L-1 ampicillin (Applichem ...
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bioRxiv - Cancer Biology 2020Quote: ... The adaptor-ligated DNA was hybridized to custom Agilent biotinylated oligonucleotide probes across a 700bp region (53032 probes; 4.684 Mbp oligo) and then pulled-down by Dynabeads M-270 Streptavidin beads (NEB). The post-capture DNA library was amplified with STARR_in-fusion_F and STARR_in-fusion_R primers (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmented samples were amplified by the addition of 2.5μL each of 10μM barcoded forward and reverse primers (Picelli et al., 2014) and 15μL Q5 2x HiFI MasterMix (New England Biolabs) using the following thermocycling conditions ...
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bioRxiv - Systems Biology 2022Quote: ... The urea concentration was then diluted to 5.5 M with 50 mM ABC and the samples digested with LysC (New England Biolabs) at 1:100 mass-ratio at 37°C for 3 hours with agitation ...
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bioRxiv - Microbiology 2022Quote: ... RNAs were precipitated in cold ethanol and 0.3 M of sodium acetate and dephosphorylated using Calf-intestinal alkaline phosphatase (New England Biolabs), according to manufacturer protocol ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μg of supercoiled plasmid DNA was incubated at 37 °C for 3 hours with purified SpRY protein at a final concentration of 1 μM and IVT gRNA (prepared without DNase treatment) at a final concentration of 2 μM in Buffer 3.1 (NEB). Reactions were stopped by the addition of 1 μL of Proteinase K (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from total RNA of 10-d-old seedlings using M-MuLV reverse transcriptase (New England Biolabs); each gene was amplified using specific primers (see Supplemental Table 2 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of each sgRNA and 60 pmol Cas9-NLS protein (EnGen® Spy Cas9 NLS, NEB, M0646 M) with the Lonza 4D X-unit ...
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bioRxiv - Genomics 2022Quote: ... 10 min) and resuspended in 400uL Buffer M supplemented with 1 mM S-adenosyl-methionine (SAM, New England Biolabs) and 200uL aliquoted as a non-methylated control ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μL of Round1 barcode mix (1x T4 DNA ligase buffer, 16 M/μL T4 DNA ligase (M0202L, NEB), 0.25 M/μL RNase Inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted from the gel overnight in 400 µl of 0.4 M NaCl with rotation at RT and precipitated with 96% ethanol in the presence of linear acrylamide (NEB).