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Citations for New England Biolabs :
651 - 700 of 1031 citations for Lassa Fever Virus GP1 Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: In vitro translation-transcription reactions were performed using the Purexpress In Vitro Protein Synthesis system (NEB #E6800). The reaction solution was prepared in the following order ...
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bioRxiv - Plant Biology 2021Quote: ... protein pellets were dissolved in 20 μl of PNGase F denaturation buffer (New England Biolabs, Ipswich, MA) and denatured at 95°C for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were separated by SDS-PAGE and detected by immunoblotting using anti-MBP (New England BioLabs, E8032S), anti-GST (MBL ...
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bioRxiv - Biochemistry 2020Quote: ... treated with lambda phosphatase protein after purification at 30°C for 1 hr (New England BioLabs, P0753L), or co-expressed in BL21 cells with RFP-lambda phosphatase ...
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bioRxiv - Biochemistry 2021Quote: 20 µg protein lysate was incubated with lambda phosphatase according to the manufacturer’s protocol (New England Biolabs). Afterwards ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of N protein was dissolved in 36 μL H218O and 2 μL 10x glycobuffer (NEB). To this solution 2 μL PNGase F was added and the reaction mixture was incubated at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were loaded directly onto the gel along with a pre-stained protein marker (New England Biolabs), and were run with a constant voltage of 60 V ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of fucose was performed using α1-2,4,5,6 fucosidase O (New England Biolabs, 2 U/μg protein), and α1-3,4 fucosidase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... His-TAD1 fusion proteins were purified according to the manufacturer’s protocol (New England Biolabs, Ipswich, MA, USA). The bait protein GST-WL1 was incubated with GST beads (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Recombinant condensin II holo(WT) at 500 nM was mixed with or without λ protein phosphatase (NEB) at a final concentration of 400 U/µL in 1x NEBuffer for Protein MetalloPhosphatases supplemented with 1 mM MnCl2 and incubated at 30°C for 60 min ...
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bioRxiv - Genetics 2021Quote: Total protein was extracted from dissociated cultured cells by incubating in 1x RIPA buffer (New England Biolabs) with added MS-SAFE Protease and Phosphatase Inhibitor (Sigma ...
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bioRxiv - Physiology 2019Quote: 40 μg of membrane protein were denaturated by adding 2 μl of denaturation buffer (New England Biolabs), for a final volume of 20 μl ...
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bioRxiv - Biophysics 2020Quote: ... proteins are recovered by digesting the DNA with the PtsI restriction enzyme (New England Biolabs, Ipswich, MA), whose cut site was incorporated into all designed oligos.
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bioRxiv - Synthetic Biology 2021Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix, New England Biolabs #E2611) into a pET-28c plasmid backbone and were designed to overexpress recombinant proteins as C-terminus His-tagged fusions ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 μg EBNA2 or 130 μg GST-fused EBNA2 protein were incubate in 1x PK buffer (NEB, 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... The assay was performed using the PURExpress In Vitro Protein Synthesis Kit (E6800S New England Biolabs, Inc.), following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... followed by adapter and motor protein ligation onto the prepared ends using NEB ligase (New England Biolabs). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... with and without the addition of ACE2 (~1:1.25 S-protein trimer:ACE2 molar ratio) (New England Biolabs). Vitrified samples of S-protein constructs with and without ACE2 were prepared by first glow discharging Quantifoil R1.2/1.3 300 mesh holey carbon copper grids for 1 minute using a Pelco easiGlow glow discharge unit (Ted Pella ...
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bioRxiv - Cell Biology 2020Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75-thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Cancer Biology 2021Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75- thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a final concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and incubated with the respective guide at 37°C for 15 min in a 1:1 ratio ...
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bioRxiv - Evolutionary Biology 2022Quote: The TF samples were expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Genetics 2023Quote: ... After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646, NEB, MA, USA), 1nL mixture was micro-injected into single-cell embryos ...
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bioRxiv - Genetics 2022Quote: ... except in the absence of phosphatase Inhibitor and incubated with 400u lambda protein phosphatase (New England Biolabs) at 30°C for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Cell Biology 2023Quote: ... 20μg protein was denatured following manufacturer’s protocol followed by PNGase F and O-Glycosidase & Neuraminidase Bundle (NEB) treatment at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Cell Biology 2023Quote: ... 1-cell stage embryos were injected with two guides for each gene with Cas9 protein (NEB, M0646T). Tyrosinase was an injection control to validate the efficacy of the Cas9 protein ...
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bioRxiv - Biophysics 2023Quote: ... concentrated protein was supplemented with 100 U TEV-His7 protease in 1X TEV protease reaction buffer (NEB) and incubated at 30 °C with agitation for 3 hr to cleave the His6-tag ...
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bioRxiv - Biochemistry 2022Quote: ... reactions were performed with 6 μl of PURExpress ΔRF123 in vitro protein synthesis system (New England Biolabs) in the presence of 1x RF3 ...
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bioRxiv - Genomics 2022Quote: ... The TF sample was expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Molecular Biology 2023Quote: MBP-Nla6 DBD was purified according to instructions in the pMALTM Protein Fusion & Purification System manual (NEB). Briefly ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... TCA extract in Laemmli buffer was diluted 10-fold in 1X PMP buffer (NEB, Protein Metallo Phosphatases) supplemented with 1 mM Mncl2 ...
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bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
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bioRxiv - Biochemistry 2024Quote: ... The strep tags were removed by incubating the proteins with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... and plasmid pQE70-TstrepSACOL2603myc-2602-2601HA-hisTspA for protein purification were created using HiFi assembly (NEB, E0554S). SACOL2603 ...
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bioRxiv - Cell Biology 2019Quote: ... then incubated with either mouse anti-MBP antibodies at a dilution of 1:5000 (New England Biolabs) or 1:2500 mouse anti-GFP antibodies (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The mouse C10 G54R mutation removes a cut site of the restriction endonuclease BanII (NEB, cat# R0119S), introducing a restriction fragment length polymorphism ...
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bioRxiv - Cell Biology 2023Quote: ... DNA isolated from lysed mouse aortic SMCs was digested with enzyme cocktail containing HindIII – XbaI – EcoRI (NEB). Following digestion ...
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bioRxiv - Cancer Biology 2023Quote: Mouse NIK coding sequence in the pENTR shuttle plasmid was mutated using Phusion high fidelity polymerase (NEB) and oligonucleotides designed to carry the desired mutations (indicated by lowercase letters) ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons from mouse embryo lysates were generated using Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 25 μL reaction ...
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bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysis was cleared by centrifugation at 16,100g for 10 min and 0.65 ml supernatant was mixed with 50μl Protein G beads (NEB, #37478S) pre-conjugated to U1-70K antibody (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... Samples were pre-cleared by adding 40 µl Protein A Magnetic Beads (New England Biolabs, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates containing 800μg of protein in a total of 400μl lysis buffer were pre-cleared by incubation with 15μl of Protein A magnetic beads (New England Biolabs) for 1h at 4°C under agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Cas9 RNPs were formed by incubating 1 pmol of sgRNA with 0.5 pmol of Cas9 protein in 1X NEBuffer™ 3.1 or 2.1 (NEB) at room temperature for 10 min ...