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Citations for New England Biolabs :
401 - 450 of 1118 citations for Lamin A C Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H3 (Lys 27) rabbit pAb (PTM Biolabs, SKU: PTM 315).
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H4 (Lys 12) rabbit pAb (PTM Biolabs, SKU: PTM 308), Butyryl-Histone H3 (Lys 9 ...
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bioRxiv - Molecular Biology 2022Quote: ... Butyryl-Histone H4 (Lys 8) rabbit pAb (PTM Biolabs, SKU: PTM 311), Butyryl-Histone H4 (Lys 5 ...
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bioRxiv - Cell Biology 2020Quote: ... samples were cooled and then digested with DpnII for one hour at 37° C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′-GGTCGCGGCCGAGGATC-3′ ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were thawed at 37°C and mixed with 20 µL of a 500U DNaseI (NEB), 10X buffer and nuclease free water mixture and incubated at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2021Quote: ... 72 °C for 5 minutes) using Phusion High-Fidelity PCR Master Mix with HF buffer (NEB), 0.1 µM of PE1.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... were incubated for 12 hours at 30°C with 7.5 Units of Phi29 polymerase (NEB M0269) in Phi29 NEB buffer 1X ...
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bioRxiv - Systems Biology 2019Quote: ... 30°C and the growth was continuously monitored online using Cell Growth Quantifier (Aquila Biolabs GmBH). Samples were withdrawn at regular intervals for analyzing the spent metabolites through High-Performance Liquid Chromatography (HPLC).
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bioRxiv - Microbiology 2019Quote: ... which was placed towards the centre of a C760 Innova – 70 °C freezer (New England Biolabs) for 1 h before being thawed in a 37 °C waterbath for approximately two min.
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bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...
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bioRxiv - Genetics 2019Quote: The product was digested overnight at 37°C using 20 U PmlI (New England Biolabs, USA), 5 uL accompanying buffer ...
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bioRxiv - Microbiology 2019Quote: ... and 4 μL was ligated at 16°C overnight with the T4 DNA ligase (NEB M0202S). After transformation into chemically-competent Top10 E ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.6) overnight at 37 °C in the presence of non-tritiated SAM (New England Biolabs). Samples were resolved via SDS-PAGE ...
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bioRxiv - Genomics 2019Quote: ... Tagmented DNA was End-Repaired for 30 minutes at 37°C (5U Klenow Exo-(NEB #M2012S), 1 × NEB Buffer 2 ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were stored at -20° C before PCR amplification of cDNA with Q5 (New England Biolabs) or SuperFiII (ThermoFischer).
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bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... after ligation overnight at 4°C with the T4 DNA ligase (New England Biolabs, Evry, France).The sequences of the cloned fragments in the resulting plasmids pYES2::MlpCSP1 ...
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bioRxiv - Microbiology 2021Quote: ... immediately rescued in 1 ml of 37°C SOC outgrowth medium (New England Biolabs, cat#B9020S) and incubated with shaking at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were incubated overnight at 60 °C with either BstNI restriction enzyme (New England Biolabs) or a control buffer solution without enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... Samples were treated twice for 45 minutes at 37°C with DNase I (New England Biolabs) with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... Individual fragments were amplified at 60°C with Phusion® High-Fidelity Polymerase (New England Biolabs) using T ...
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bioRxiv - Physiology 2021Quote: ... The pNB1u vector was digested overnight at 37 °C with PacI and Nt.BbvCI (New England Biolabs) to generate 8-nt overhangs ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C) and the supernatant was added to 24 mL of amylose resin (NEB, Ipswich MA) pre-equilibrated with buffer C and nutated for 2 hr at 4°C ...
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bioRxiv - Genomics 2021Quote: ... followed by an overnight incubation at 16°C with 3 µL T4 DNA ligase (NEB, M0202). Samples were purified with phenol-chloroform and used as 3C templates for Taqman-qPCR ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were treated with proteinase K at 37 °C for 10 min (New England Biolabs) and the RNA samples were purified by RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 37°C into pPB_U6::gRNA_EF1a::BFP-Puro digested with BlpI (NEB #R0585S) and BstXI (NEB #R0113S) ...
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bioRxiv - Genomics 2019Quote: ... samples were cooled and then digested with DpnII for one hour at 37°C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′ -GGTCGCGGCCGAGGATC-3′ ...
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bioRxiv - Cell Biology 2020Quote: ... pFA6a-mto2S338N-C-KanMX6 was generated using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs) in an assembly containing 5 fmol of each fragment ...
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bioRxiv - Biochemistry 2022Quote: In vitro coupled transcription-translation was performed at 37 °C using the PURExpress ΔIleRS Kit (NEB) following manufacturer’s protocol supplemented with [35S]-methionine (EasyTag ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid and insert were then ligated overnight at 16 °C (T4 DNA ligase, New England Biolabs). After transformation into chemically competent E.coli and subsequent plasmid purification ...
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bioRxiv - Plant Biology 2023Quote: ... 10 ul of diluted DNA were used for McrBC digestion (NEB, 4 h at 37 °C) or mock digestion (the same volume of H2O instead of McrBC enzyme was added with all other components the same in the reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... for 2 hours at 37°C in 1X T4 PNK buffer (New England Biolabs, cat#B0201S). Radioactively labeled tRNAs were produced by T7 in vitro transcription in the presence of [α-32P]-UTP (PerkinElmer ...
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bioRxiv - Genomics 2022Quote: ... cells were digested for 2 hours at 37°C using 375U of MboI (NEB, Cat. #R0147). After biotin filling ...
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bioRxiv - Microbiology 2023Quote: ... Vector and insert were ligated overnight at 16°C using T4 DNA ligase (New England Biolabs) and transformed into E ...
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bioRxiv - Cancer Biology 2023Quote: ... Hi-C single-index library preparation was performed as previously described56 using MboI (New England Biolabs) restriction enzyme.
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bioRxiv - Microbiology 2023Quote: ... Total RNA was then digested using DNase I for 2h at 37°C (New England Biolabs) to remove residual plasmid DNA and then purified using Monarch RNA cleanup kit (New England Biolabs) ...
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bioRxiv - Biochemistry 2023Quote: ... per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs) plus 1 mM MnCl2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the P1-adaptors and Sbf I-HF (New England Biolabs)-digested DNAs for 90 min at 37°C were ligated by T4 ligase (New England Biolabs). After DNA pooling ...
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bioRxiv - Cell Biology 2023Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology)6 sequence in-frame with the KRT5 sequence separated by a short peptide linker (DPAFLY) ...
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bioRxiv - Cell Biology 2023Quote: ... 45-72°C for 30 seconds (depending on melting temperature of primer pairs determined by NEB Tm calculator https://tmcalculator.neb.com) ...
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bioRxiv - Genomics 2024Quote: ... The Hi-C libraries were amplified for 11–15 cycles with Q5 master mix (NEB, M0492L) following the operation manual ...
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bioRxiv - Genomics 2024Quote: ... followed by proteinase K treatment (New England Biolabs, #8102S 20µg/µL, 1.5 h at 55°C). DNA was eluted in Milli Q water ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were probed with rabbit polyclonal anti-pansuccinyllysine antibody (PTM Biolabs, PTM-401) at a dilution of 1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Using 10 μg of custom-made rabbit polyclonal anti-AcK142 Ab (Ez Biolabs), AcK142 PNKP was IP’d from 1 mg of GO-treated chromatin fraction from WT-PNKP-FLAG and K142R-PNKP-FLAG expressing cells ...
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bioRxiv - Microbiology 2021Quote: ... Ligation to each splint was performed overnight at 16°C using T4 DNA ligase (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then incubated at 45 °C for 2 hr with 5 μL of Proteinase K (NEB) in the presence of 40 mM EDTA (pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Digested chromatin was then ligated at 16 °C with 10000 U of T4 DNA ligase (NEB, M0202) in ligase buffer supplemented with 10% Triton-100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... padlock probe ligation was performed overnight O/N at 25°C using the SplintR ligase (NEB, M0375) at a final concentration of 0.5 Units/μl ...