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Citations for New England Biolabs :
1 - 50 of 332 citations for LS Tetrasaccharide a LSTa Sialyl lacto N tetraose a >90% NMR Sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... goat polyclonal anti-UIS4 (LS Biolabs LS-C204260; IFA 1:1000); rabbit polyclonal anti-LISP2 (IFA ...
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bioRxiv - Cell Biology 2023Quote: ... Free nucleotides were purchased as 100mM stocks of dATP or dUTP as sodium salts in ultrapure water (NEB) and stored at -20°C ...
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bioRxiv - Biophysics 2021Quote: ... of 0.1M sodium hydroxide: 0.02M 2-(n-morpholino) ethanesulfonic acid (MES) or 1X NEB DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Bioengineering 2021Quote: ... 90 nM of SpCas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Genomics 2024Quote: ... 90 nM of SpCas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... 90 nM of Cas9 nuclease (NEB), Cas9 nuclease buffer (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 90 nM of SpCas9 protein (New England Biolabs), Cas9 nuclease buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... 90 μL dH2O and +/− 2500 U PNGase F (NEB) was added to each tube and incubated at 37 °C for 1.5 h ...
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bioRxiv - Genetics 2020Quote: ... 90 nM of SpCas9 protein (#M0386, New England Biolabs), NEB buffer 3.1 ...
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bioRxiv - Immunology 2021Quote: ... 90 nM of EnGen® Sau Cas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were diluted in 90 μl Elution Buffer (NEB), mixed with 120 μl AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2020Quote: ... sodium orthovanadate (NEB, 1mM), and sodium fluoride (NEB ...
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bioRxiv - Physiology 2022Quote: ... sodium orthovanadate (NEB, 1mM), sodium fluoride (NEB ...
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bioRxiv - Microbiology 2024Quote: ... according to the supplieŕs instructions (GPSLM-LS Linker-Scanning System, New England Biolabs). The insertion of the Tn7 based transprimer into the target plasmid produced two classes of hybrid plasmids containing the transprimer in either the vector backbone or in the target gene ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 400 U of Salt-T4 DNA Ligase (NEB). All reactions were then incubated at 37 °C 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 8 U/μl Salt-T4 DNA ligase (NEB #M0467)) ...
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bioRxiv - Physiology 2022Quote: ... sodium fluoride (NEB, 1 mM), and sodium butyrate (100uM) ...
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bioRxiv - Systems Biology 2021Quote: ... for 90 minutes and then ligated sequencing adaptors with T4 ligase (NEB) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mM Vanadate (Sodium Orthovanadate, NEB, pre-incubated for ten minutes at 95 °C to dissociate Vanadate oligomers ...
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bioRxiv - Genetics 2020Quote: ... and sodium fluoride (NEB, 1 mM). 150uL of ice cold buffer was added to heads followed by immediate homogenization with a motorized pestle for 10 seconds on ice ...
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bioRxiv - Microbiology 2020Quote: ... in 0.5 x TBE for 60-90 min alongside 100 bp ladder (NEB). Gels were stained for 30 minutes in ethidium bromide solution before imaging under UV-light.
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bioRxiv - Immunology 2024Quote: ... and β-N-Acetylglucosaminidase S (NEB), or all three enzymes were performed overnight at 37 °C using 1 unit each of enzyme ...
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bioRxiv - Cell Biology 2020Quote: ... or sodium orthovanadate (Na3VO4, 1 mM; NEB) to test dephosphorylation of CatSper1 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 5 mM sodium orthovanadate (New England Biolabs), and 10 mM sodium fluoride (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... containing 1 mM sodium orthovanadate (NEB, # P0758S). The membrane from the transwell inserts was cut using forceps and placed in 300 µL of 1X cell lysis buffer (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... phosphatase inhibitors (Sodium Orthovanadate, New England BioLabs, P0758 ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and de-N-glycosylated using 500 U N-glycosidase F (PNGase F, New England Biolabs) for 12 h at 37°C ...
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bioRxiv - Genomics 2023Quote: ... RNAs were 5’dephosporylated through 90 minutes incubation in total with thermostable QuickCIP (NEB) in which the samples were briefly heated to 75°C and quickly chilled on ice at the 60 minutes mark ...
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bioRxiv - Genomics 2024Quote: ... RNAs were 5’dephosporylated through 90 minutes incubation in total with thermostable QuickCIP (NEB) in which the samples were briefly heated to 75°C and quickly chilled on ice at the 60 minutes mark ...
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bioRxiv - Neuroscience 2023Quote: ... N-glycans were released using N-glycanase PNGase F (1239U/ml, New England BioLabs, Inc. cat no. P0709L) and were fluorescently labelled with 2-aminobenzamide (2-AB ...
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bioRxiv - Molecular Biology 2023Quote: ... while wheat AGPase LS was cloned into pUC-SYCE with the help of Gibson assembly master mix (NEB), using BamHI as restriction enzyme (Sharma et al. ...
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bioRxiv - Bioengineering 2022Quote: ... or 1 μg Asp-N (NEB, England) for overnight digestion respectively ...
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bioRxiv - Immunology 2020Quote: ... deglycosylated with N-glycanase (New England Biolabs), and digested overnight with LysC protease (ThermoFisher scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... or N-glycosilase F (PNGaseF, NEB P0704S), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... endoproteinase Asp-N (New England Biolabs, #P8104S), Glu-C (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Sodium Orthovanadate (New England Biolabs, Cat. no. p0758s), Pepstatin (Cat ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 0.5% sodium dodecyl sulfate) and Proteinase K (NEB). Reactions were incubated at room temperature overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and sodium fluoride (New England Biolabs; item # P0759). Western blotting was performed using a sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was then extracted from washed beads using Trizol LS and treated with T4 Polynucleotide Kinase (New England Biolabs) to obtain 5’ phosphate ends for subsequent ligations and passed through NucAway columns (Ambion ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsin digest of PDI was followed by digestion of N-glycans with endo-β-N-acetylglucosaminidase H (500 U; NEB) in sodium citrate buffer (50 mm ...
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bioRxiv - Microbiology 2024Quote: Codon-optimized full-length SARS-CoV-2 S protein gene was cloned into Topo cloning vector and 22 N-glycosylation sites were mutated individually from N to Q using a Q5 Site-Directed Mutagenesis Kit (NEB). The full-length or C19 truncated fragments were amplified and inserted into plvx-puro vector ...
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bioRxiv - Microbiology 2022Quote: ... Peptide-N-Glycosidase F (PNGase F; NEB, P0704) treatment of lysates was performed as described in the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2023Quote: ... coli BL21 lysY/Iq (NEB cat n° C3013). The expression plasmid was transformed via heat-shock followed by selection of clones on LB-Agar plates supplemented with 100 µg/mL carbenicillin ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the P1-adaptors and Sbf I-HF (New England Biolabs)-digested DNAs for 90 min at 37°C were ligated by T4 ligase (New England Biolabs). After DNA pooling ...
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bioRxiv - Cell Biology 2022Quote: ... Sodium Orthovanadate (Vanadate) was from New England BioLabs (NEB), catalase and hydrogen peroxide (30 % ...
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bioRxiv - Biophysics 2021Quote: ... and Sodium Orthovanadate (New England BioLabs, Ipswich, MA, USA). Tissue was homogenized using an electric homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were amplified for N-1 cycles (being N the optimum Cq determined by qPCR reaction) using NEBNext High-Fidelity Polymerase (New England Biolabs, M0541). Libraries were purified with Sera-Mag Select Beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... pHAGE lentiviral plasmids encoding the six other HCoV N-EGFP were generated by replacing SARS-CoV-2 N with the respective HCoV N sequences by PCR (New England Biolabs M0492S) and NEBuilder HiFi DNA Assembly (New England Biolabs E2621S) ...
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bioRxiv - Microbiology 2024Quote: SARS-CoV-2 N (nucleocapsid) protein qPCR system (IDT, Iowa City, IA) and RSV N qRT-PCR (Luna, NEB, Ipswich, MA) were detected under one-step qRT-PCR on a QuantStudio 3 (Applied Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... the pHAGE lentiviral plasmid encoding SARS-CoV-2 N-LgBIT and N-SmBIT were generated by NEBuilder HiFi DNA Assembly (New England Biolabs E2621S). Oligonucleotide primers were obtained from Azenta Life Sciences ...