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Citations for New England Biolabs :
251 - 300 of 865 citations for Kinase Domain of Pyruvate Kinase liver Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and end-labeled using T4 polynucleotide kinase (M0201, NEB) and [γ-32P] ATP (NEG002A250UC ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 poly nucleotide kinase (New England Biolabs, M0201L) and then ligated with 3ʹ RNA linker (with sample barcodes ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 10 µL ...
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bioRxiv - Biophysics 2021Quote: ... The E1371Q samples were phosphorylated using protein kinase A (NEB). The wild-type sample was de-phosphorylated using λ-phosphatase ...
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bioRxiv - Genetics 2021Quote: ... and 10 U polynucleotide kinase (New England Biolabs, Beverly, MA) as instructed by the manufacturer ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... and then modified using T4 Polynucleotide Kinase (New England Biolabs), which removes the 3’-phosphate and adds 5’-phosphates ...
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bioRxiv - Genetics 2019Quote: ... followed by treatment with T4 polynucleotide kinase (New England BioLabs) as described previously53 ...
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bioRxiv - Cancer Biology 2020Quote: ... annealed oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, M0201S) and cloned into BsmBI-linearized ...
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bioRxiv - Genomics 2019Quote: ... the RNA products were treated with Polynucleotide Kinase (PNK, NEB) at 37°C for 30 min.
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bioRxiv - Molecular Biology 2020Quote: ... Telomere probe was labeled using T4 polynucleotide kinase (NEB M0201) and <ι>γ-P32-ATP (Perkin Elmer NEG035C ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with 8 μl of T4 Polynucleotide Kinase (NEB) and 2 μl of 10 mM ATP for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the oligos were phosphorylated by T4 Polynucleotide Kinase (M0201S, NEB) at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... followed by phosphorylation with T4 polynucleotide kinase (New England Biolabs). The vector pPEPZ-sgRNAclone was digested with BsmBI and carefully purified by gel extraction to ensure removal of the mCherry fragment ...
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bioRxiv - Neuroscience 2020Quote: ... Ca2+/calmodulin-dependent protein kinase II (CaMKII, New England Biolabs).
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 µ l of T4 Polynucleotide Kinase (NEB, M0201L). Following incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant kinases (2500 U/mg protein for PKA (NEB-P600S), 500:1 Tau:kinase for Gsk3ß (BPS-40007) ...
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bioRxiv - Neuroscience 2022Quote: ... and 1x NEB-uffer for Protein Kinases (New England Biolabs). The samples were then subjected to protein precipitation ...
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bioRxiv - Microbiology 2021Quote: ... and 0.4 μl of T4 polynucleotide kinase (New England Biolabs). The THN gene was amplified with the phosphorylated primers MR161-MR162 using Phusion Flash High-Fidelity PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: The annealing reaction was performed using T4 polynucleotide kinase (NEB) under the following conditions ...
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bioRxiv - Biochemistry 2019Quote: Kinases were treated with 0.5µL lambda phosphatase (New England Biolabs) per 1µg protein for 1 hour at 30°C in PMP buffer (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... RNA fragments were further dephosphorylated using T4 polynucleotide kinase (NEB) for 1h at 37°C followed by heat inactivation for 10min at 75°C ...
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bioRxiv - Microbiology 2019Quote: ... The strands were end phosphorylated by T4 polynucleotide kinase (NEB) and annealed ...
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bioRxiv - Microbiology 2019Quote: ... The strands were end phosphorylated by T4 polynucleotide kinase (NEB), annealed ...
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bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... using a standard T4 polynucleotide kinase labeling protocol (M0201L, NEB). Each binding reaction (10 μL sample volume ...
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bioRxiv - Biochemistry 2021Quote: 5’ phosphorylation of micro HHrz RNA with polynucleotide kinase (NEB) as done following manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... the remaining RNA was T4 Polynucleotide Kinase (New England Biolabs) treated ...
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bioRxiv - Genetics 2020Quote: ... and phosphorylated with T4 Polynucleotide Kinase (New England Biolabs, NEB), ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Genetics 2020Quote: ... and phosphorylated with T4 Polynucleotide Kinase (New England Biolabs, NEB), ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Amplified constructs were treated with DpnI and polynucleotide kinase (NEB) for 10 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... and T4 Poly Nucleotide Kinase (10 U) (New England Biolabs) in a total volume of 30 μL PNK buffer A (1x) ...
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bioRxiv - Microbiology 2022Quote: ... and end-labelled with T4 polynucleotide kinase (New England Biolabs). This fragment was then digested for 2 h with MfeI at 37°C in a reaction volume of 60 μl ...
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bioRxiv - Plant Biology 2023Quote: ... T4 Ligase and T4 polynucleotide kinase was obtained from NEB. Plasmids were isolated using the alkaline lysis method [15] ...
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bioRxiv - Molecular Biology 2023Quote: ... Phosphorylations and ligations were performed using T4 PNK kinase (NEB) and T4 DNA ligase (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... and 10 U of T4 Polynucleotide Kinase (New England Biolabs) in a final reaction volume of 15 μl at 37°C for 1.5 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... phosphorylated using T4 polynucleotide kinase (New England Biolabs, Ipswich, MA), and ligated into digested BsmBI-digested (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... and the primers are phosphorylated using polynucleotide kinase prior (NEB) to PCR such that each PCR product strand can be immediately ligated to itself ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5′-hydroxyl group repair (T4 polynucleotide kinase (M0201L; NEB) were conducted on beads ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 units T4 Polynucleotide Kinase (PNK, New England Biolabs, M0201S), 1x T4 PNK buffer ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of protein kinase buffer (New England Biolabs) with 3 μCi [γ-32P]ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was incubated with 1 U T4 polynucleotide kinase (NEB) in presence of its buffer (70 mM tris-HCl pH 7.6 ...
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bioRxiv - Genomics 2024Quote: ... gRNAs were phosphorylated with a T4 Polynucleotide Kinase (NEB, M0201) for 30 minutes at 37°C followed by inactivation at 95°C for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The 5’-phosphate was added via T4 polynucleotide kinase (NEB) at 37 ºC for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by phosphorylation of the 5’ end with T4 kinase (NEB). Phosphorylated RNA was then purified by the RNeasy kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... after which 5’end was repaired with T4 polynucleotide kinase (NEB). Nascent RNAs containing the adaptors were converted to cDNA ...
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bioRxiv - Systems Biology 2022Quote: ... and 0.5 μl of T4 polynucleotide kinase (NEB cat. no. M0201S), incubating as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... The probes were labeled using T4 polynucleotide kinase (New England BioLabs) and [γ-32P] ATP (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ ends were phosphorylated by 20 U polynucleotide kinase (PNK; NEB) by adding 1 mM ATP (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: Ric-8A was phosphorylated using casein kinase II (New England Biolabs) as previously described10 ...