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Citations for New England Biolabs :
1 - 50 of 98 citations for Ketoconazole EP Impurity D since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... pYES2sh was first linearized with the restriction enzymes NotI and EcoRI and amplified Ep-CoV2 or Ep-CoV2™ DNA fragments was inserted into the vector backbone using NEBuilder® HiFi DNA Assembly (NEB, Ipswich, MA, USA). The viral potassium channel KcvPBCV-1 was used as a positive control in the yeast complementation assay [36] ...
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bioRxiv - Bioengineering 2021Quote: ... EP-PCR was performed using Taq polymerase and the standard Taq buffer (New England Biolabs) with imbalanced dNTP (0.2 mM dATP ...
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bioRxiv - Neuroscience 2020Quote: ... Trimmed gel pieces were transferred to an EP tube and digested with Proteinase K (NEB, P8107) at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... Random mutagenesis was performed using Error-Prone PCR (EP-PCR) with Taq DNA polymerase (New England Biolabs). PCR products and restriction digestion products were purified using a GeneJET gel extraction kit (ThermoFisher) ...
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bioRxiv - Pathology 2021Quote: ... The amplified Ep-CoV2 DNA fragment was then inserted into the vector backbone using NEBuilder® HiFi DNA Assembly (NEB, Ipswich, MA, USA). For Western blot analysis ...
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bioRxiv - Pathology 2021Quote: ... The DNA fragment encoding the desired FLAG-TEV tag was generated via PCR using primer pair 2 (table 1) and subsequently cloned into the NdeI restriction site of pET24Δlac/Ep-CoV2 using NEBuilder® HiFi DNA Assembly (NEB, Ipswich, MA, USA).
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bioRxiv - Genomics 2020Quote: ... oligo d(T)23VN primer (NEB) was annealed to template RNA ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress D Ribosome Kit (NEB #E3313S) and New England Biolabs PURExpress In Vitro Protein Synthesis Kit (NEB #E6800) ...
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bioRxiv - Microbiology 2021Quote: ... 50 pmol Oligo d(T)23 (NEB) and 10 pmol Deoxynucleotide Triphosphate Mix (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... Magnetic oligo-d(T) beads (NEB, S1419S) were equilibrated in NLB and added to the enrichments ...
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bioRxiv - Microbiology 2024Quote: ... using oligo d(T)23 VN (NEB) primers.
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bioRxiv - Biophysics 2024Quote: ... In the first double-digestion reaction two DNA fragments of 3,621 nt (D) and 3,628 nt (D’) were generated using DraIII and BaeGI enzymes (New England Biolabs; Figure S1a). The digestion reaction involved annealing two oligonucleotides (Figure S1b ...
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bioRxiv - Immunology 2024Quote: ... and 1.2 nmol of the puromycin linker (5’-/5Phos/-d(A)21-(C9)3-d(ACC)-puromycin-3’) by the T4 DNA ligase (New England Biolabs) in a 100 µL reaction for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... using Oligo d(T)23 VN-S1327S (NEB), dNTPs 10mM ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μg of biotinylated RNA was subjected to oligo d(T) selection using oligo d(T)25 magnetic beads (NEB S1419S), substituting SDS and NaCl for the recommended LiDS and LiCl ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We first PCR-amplified the egfp-hiphop coding sequences (either the D. melanogaster or D. yakuba allele) using Phusion High-Fidelity DNA Polymerase (NEB, Ipswich, MA) from the HDR plasmids (see above) ...
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bioRxiv - Cell Biology 2021Quote: ... and oligo d(T)23 primer (New England Biolabs) for transcripts ...
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bioRxiv - Microbiology 2021Quote: ... and 2U of Uracil-D-glycosylase (New England Biolabs, USA). Conditions of PCR were as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... oligo-d(T)25-Magnetic Beads (S14195, New England Biolabs) were used ...
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bioRxiv - Microbiology 2023Quote: ... mRNA molecules are captured using magnetic Oligo d(T)beads (NEB). Following purification ...
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bioRxiv - Genetics 2024Quote: ... One microliter of oligo d(T)23VN primer (50 μM) (NEB) was added to 200 ng/µL of RNA (170 ng worm RNA + 35 ng yeast RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... mRNAs were enriched using Oligo d(T)25 Magnetic Beads (NEB S1419S) following manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... mRNA was enriched with Oligo d(T)25 Magnetic Beads (New England Biolabs) and fragmented by heating at 94 °C for 3 min in 5× First Strand Buffer (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 15 µl of Oligo d(T)25 Magnetic Beads (S1419S, New England Biolabs), were combined with 50 µl of binding buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... pGEMHE-DEST containing TaHKT1;5-D was linearized using sbfI-HF (New England Biolabs) before synthesising cRNA using the mMESSAGE mMACHINE T7 Kit (Ambion ...
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bioRxiv - Genomics 2021Quote: ... samples were incubated with 20 μl Oligo d(T) Magnetic Beads (NEB, S1419S) in PCR strips for selection of polyadenylated (poly(A) ...
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bioRxiv - Genomics 2021Quote: ... mRNAs were incubated with Oligo d(T) Magnetic Beads (New England BioLabs S1419), then fragmented in 2x Superscript III first-strand buffer (Thermo Fisher Scientific with 10mM DTT (Thermo Fisher Scientific 18080044 ...
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bioRxiv - Genomics 2022Quote: ... mRNA was isolated using Oligo d(T)25 Magnetic Beads (New England Biolabs) and fragmented ...
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bioRxiv - Genomics 2022Quote: ... mRNAs were enriched by incubation with Oligo d(T) Magnetic Beads (NEB, S1419S) and then fragmented/eluted by incubation at 94°C for 9 min ...
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bioRxiv - Immunology 2024Quote: ... mRNAs were enriched by incubation with Oligo d(T)25 Magnetic Beads (NEB). To fragment Poly A-enriched mRNA ...
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bioRxiv - Immunology 2020Quote: PBMCs were co-emulsified with oligo d(T)25 magnetic beads (New England Biolabs) in lysis buffer (100mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... samples were incubated with 20 μl Oligo d(T)25 Magnetic Beads (NEB, S1419S) in PCR strips for selection of polyadenylated (poly(A) ...
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bioRxiv - Genomics 2024Quote: ... Poly(A) mRNA was selected using Oligo d(T)25 Magnetic Beads (NEB, S1419S) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: PBMCs were co-emulsified with oligo d(T)25 magnetic beads (New England Biolabs) in lysis buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The pENTR/D construct was then digested with the MluI restriction enzyme (NEB, Cat# R0198S) and recombined into a modified gateway destination vector harboring Hygromycin drug resistance as described in Law et al ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 µg of total RNA was used with D(T)23VN primer (New England Biolabs), 10 mM dNTPs (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... Poly(A)+ RNA was purified using Oligo d(T)25 Magnetic Beads (New England Biolabs). For northern blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... and WT SETD8 or T140A/D SETD8 cDNA were ligated using T4 ligase (NEB #M0202). T140A/D SETD8 cDNAs were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit according to the manufacturer’s instructions (Agilent #210518).
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bioRxiv - Genomics 2020Quote: ... Further processing for mRNA selection was performed with Oligo-d(T)25 Magnetic Beads (NEB, E7490) and the integrity of the RNA was validated once more with Agilent 4200 TapeStation (Agilent ...
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bioRxiv - Genomics 2021Quote: ... the rest was subjected to pull-down with oligo-d(T)x25 magnetics beads (NEB-S1419S), 1ml of slurry per 1L of cell culture during 1 hour at 4°C ...
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bioRxiv - Genomics 2022Quote: ... polyadenylated RNA was isolated from total RNA using oligo d(T)25 Magnetic Beads (NEB, #S1419S) at a 1μg Total RNA to 10μg beads ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... and the D-AlaR cassette (pGGF003) were combined in a ’Greengate reaction’ using BsaI-HF (NEB), T4 DNA-Ligase (Thermo Scientific) ...
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bioRxiv - Genomics 2022Quote: ... and mRNA was selected using 75 μl of Oligo d(T)25 magnetic beads (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... We extracted RNA using Monarch® Total RNA Miniprep Kit (New England BioLabs GmbH, Frankfurt, D, E2010). Material was ground in Mixer Mill 200 (Retsch GmbH ...
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bioRxiv - Plant Biology 2022Quote: ... and 3xHA::3C::BLRP tag encoding DNAs were respectively ligated into pENTR/D constructs using AscI (NEB) digestion site in the pENTR/D vector ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were treated with JET (12.5 nM d-o-p) and/or ScaI-HF (NEB, 5 U) for 15 minutes at 37°C ...
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bioRxiv - Genomics 2024Quote: ... 30 μg per replicate of poly-adenylated mRNA was isolated using oligo d(T) beads (NEB E7490L). Resulting mRNA was split into 6 separate reactions and cDNA was synthesized using a gene-specific primer (5’-CTCATCAATGTATCTTATCATGTCTG-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... Poly(A)+ RNAs were enriched from total RNAs using Oligo d(T)25 magnetic beads (New England Biolabs), according to the manufacturer’ s instructions ...
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bioRxiv - Immunology 2020Quote: The second PCR product (V, D, J heavy genes) was cloned in frame by HiFi assembly (NEB, UK) into the KpnI/PstI (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... This was followed by one round poly(A) purification using oligo d(T) magnetic beads (New England Biolabs). 1.5-2 µg of mRNA was fragmented to 100-150 nts using RNA Fragmentation Reagent (Thermo Fisher Scientific ...