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Citations for New England Biolabs :
51 - 100 of 3501 citations for Importin subunit alpha 1 KPNA2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... coli strains NEB 5-alpha F’lq (New England Biolabs, US) and MC1061 with plasmid pKD46 which carries ARA-inducible Red recombination system24 ...
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bioRxiv - Systems Biology 2021Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB) and grown in liquid culture ...
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bioRxiv - Systems Biology 2021Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB) and grown in liquid culture.
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bioRxiv - Biophysics 2022Quote: ... Toxicity-resistant strains such as NEB 5-alpha F’Iq (NEB) and ABLE K (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... coli dam+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/ dcm− (dam−/dcm− competent E ...
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bioRxiv - Biochemistry 2022Quote: ... T4 DNA ligase and chemically competent DH5 alpha cells (NEB); Alt-R CRISPR-Cas9 crRNA ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5-alpha cells following manufacturer’s protocol (New England Biolabs). Sanger sequencing was used to confirm plasmid construction ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEB® 5-alpha F’IQ (New England Biolabs, NEB) were used for routine cloning procedures and chemically competent E ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEB® 5-alpha F’IQ (New England Biolabs, NEB) were used for routine cloning procedures and chemically competent E ...
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bioRxiv - Synthetic Biology 2022Quote: ... Assembled products were heat-shock transformed to 5-alpha (NEB), TOP10 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
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bioRxiv - Genomics 2023Quote: ... The library was transformed into 5-alpha electrocompetent cells (NEB), grown in liquid culture ...
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bioRxiv - Genomics 2023Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB). The plasmid library was prepared by maxi-prep (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Escherichia coli competent cells NEB 5-alpha (C2987, New England Biolabs), NEB Stable (C3040 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were transformed into either competent NEB 5-alpha F’Iq (NEB) or Epi400 (Lucigen) ...
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bioRxiv - Molecular Biology 2022Quote: ... and were performed using 5-alpha F’ Iq cells (NEB C2992H).
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bioRxiv - Biochemistry 2021Quote: ... The assembled product was transformed into 5-alpha competent cells (NEB) and the insert sequence was confirmed by Sanger sequencing (Source Bioscience) ...
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bioRxiv - Developmental Biology 2022Quote: ... it was transformed into NEB 5-alpha competent E.coli (NEB #C2987). The sequence of donor plasmid was confirmed by Sanger sequencing using 5 primers to ensure that there are no disabling mutations in the scarless DsRed cassette or regions of the homology arms ...
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bioRxiv - Immunology 2022Quote: ... and then transformed in NEB 5-alpha competent cells (NEB, C2987U). Expi293F (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... coli host strain NEB 5-alpha F’ lq (New England Biolabs) was exclusively used to generate ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was transformed into NEB 5-alpha cells (New England Biolabs) and plated on LB plates with carbenicillin for selection purposes and incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and then transformed into 5-alpha competent Escherichia coli cells (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes with a spytag labeled L17 subunit was used during in vitro translation system (NEB, E3313S) to generate the stalled RNC ...
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bioRxiv - Biophysics 2022Quote: ... 2018) and a C-terminal 6x His tag and cloned into the pET21B vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Cell Biology 2022Quote: ... pET17b-Kif5b(1-560)-GFP-His was transformed into BL-21[DE3]RIPL cells (New England Biolabs) until an optical density at 600 nm of 0.6-0.8 and expression was induced with 0.5 mM isopropyl-β-d-thiogalactoside (IPTG ...
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bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB 5-alpha (fhuA2∆(argF-lacZ)U169 phoA glnV44 ϕ 80∆(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17) (New England Biolabs) was used for the initial two-plasmid assay ...
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bioRxiv - Microbiology 2024Quote: ... Single-construct plasmids expressing A-subunits were constructed via restriction digestion (NdeI and PstI, New England Biolabs) and ligation using pBAD24 (Amp+ ...
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bioRxiv - Microbiology 2021Quote: ... Clones were selected and maintained in E.coli strain NEB5-alpha (NEB, C2987I). All constructs were verified by Sanger sequencing (Eurofins) ...
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bioRxiv - Microbiology 2021Quote: ... Ligations were used to transform NEB 5-alpha competent cells (NEB C2987H) and the cloned spacer was verified by Sanger sequencing using primer PSP108 ...
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bioRxiv - Microbiology 2021Quote: ... Assemblies were transformed into DH5-alpha chemically competent cells (New England Biolabs) and correct clones were identified by restriction profile and Sanger sequencing (Genewiz ...
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bioRxiv - Molecular Biology 2020Quote: ... transformed into NEB 5-alpha chemically competent Escherichia coli (New England BioLabs), and submitted for Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2020Quote: ... DNA was transformed into NEB 5-alpha high efficiency competent cells (NEB). Insert size was verified with PCR and purified plasmids were sequenced using Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Systems Biology 2023Quote: ... Each construct was transformed into standard 5-alpha competent bacteria (#C2987; NEB) grown overnight in in 500 ml of standard Luria Broth (LB ...
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bioRxiv - Molecular Biology 2023Quote: ... coli competent cells (NEB 5-alpha, New England Biolabs, Ipswich, MA, USA) that were cultured and prepared using a GenElute HP Plasmid Midi kit (NA0200-1KT ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of human recombinant histone H4 (BioLabs, Cat# M2504S) was incubated with 50 μM n-butyryl- or isobutyryl-CoA and 0.2 μM of HAT1 at 30 °C for 1 hour ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was cut with Hind III and Bam HI (NEB) for 1h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and His-MBP-ZFC3H1 onto an Amylose resin (NEB). After extensive washing ...
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bioRxiv - Immunology 2021Quote: ... were assembled using Hi-Fi DNA Assembly Mix (NEB). For the SFFV-Cas9 vector ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10µg/ml) and apyrase (25mU/ml, NEB); the suspension was incubated for 1h at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... or Hi-Fi assembly from New England BioLabs (NEB) and other basic molecular cloning approaches ...
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bioRxiv - Genomics 2021Quote: ... was then inserted by Hi-Fi assembly (NEB, E2621) using 0.025 pmols of vector and 0.05 pmols of the GFP amplicon ...
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bioRxiv - Cell Biology 2021Quote: ... using NEB Hi-Fi assembly mix (New England Biolabs). A mixture of the two guide plasmids (each at 100ng/µl ...
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bioRxiv - Developmental Biology 2023Quote: ... or Q5 Hi-Fidelity DNA Polymerase (New England Biolabs) was used ...
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bioRxiv - Biochemistry 2024Quote: ... We expressed the His-SUGCT in Lemo cells (NEB), inoculating 4L of TB with 4 mL overnight culture ...
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bioRxiv - Neuroscience 2019Quote: ... was first transformed into NEB 5-alpha competent cells (New England Biolabs #E2621S) and plated on LB plus ampicillin (60 µl/ml).