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Citations for New England Biolabs :
151 - 200 of 3961 citations for Huntingtin Interacting Protein 1 Related Protein HIP1R Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Microbiology 2021Quote: One microgram of full-length S protein was treated with 1 μg of FXa (P8010L, NEB), furin (P8077S ...
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bioRxiv - Biochemistry 2023Quote: ... per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs) plus 1 mM MnCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... All commercially sourced enzymes and related buffers were purchased from New England Biolabs (NEB, Ipswich, MA, USA) unless otherwise indicated ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutagenesis and related PCR reactions were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, #M0491). The mutant A3G library was constructed via multi-site saturation mutagenesis of codons 128 and 130 using NNS degenerative codons (N = A ...
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bioRxiv - Microbiology 2020Quote: ... Cloning and purification of full length IE86 protein: Full length IE86 protein was codon optimized and cloned in pMALcx5 vector (New England Biolabs Inc.). Cells were transformed in BL-21 cells and IE86-FL-pMALcx5 transformed cells were grown in 1L luria broth (Thermofisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Phosphorylation of purified LPR1 protein was tested according to (Maldonado-Bonilla et al., 2014) using λ-protein phosphatase (New England Biolabs). In brief ...
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bioRxiv - Genetics 2022Quote: ... along with 6uL of EZ-Run Pre-Stained Rec Protein Ladder (Fisher BP3603-500) and 3uL of Blue Prestained Protein Standard (NEB P7718S). Gels were electrophoresed in XT Tricine Running Buffer (Bio-Rad 1610790 ...
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bioRxiv - Synthetic Biology 2023Quote: The cell-free protein synthesis (CFPS) reactions were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc) in 384-well plates (Thermofisher NUNC ...
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bioRxiv - Microbiology 2023Quote: RdnE proteins were produced using the New England Biolabs PURExpress In Vitro Protein Synthesis Kit (New England BioLabs Inc., Ipswich MA). Template DNA contained the rdnE gene and required elements specified by the PURExpress kit ...
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bioRxiv - Cell Biology 2023Quote: ... a PCR fragment containing the complete Nv-osk coding sequence was synthesized for protein expression with PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) using manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... To remove any remaining phosphorylation the GFP-Trap bound proteins were treated with 40 units of Lambda Protein Phosphatase (New England Biolabs, P0753S) at 30°C for 30min ...
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bioRxiv - Microbiology 2020Quote: ... glabrata total cell lysates using the anti-Rad53 antibodies described in the previous section and Protein A magnetic beads (New England Biolabs). The immunoprecipitated samples were run on 8% acrylamide gels ...
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bioRxiv - Physiology 2020Quote: ... 1 nl of CRISPR mixture containing 2,4 μg/μl of gRNA and 0.5 μl Cas9 protein (NEB) was injected in one-cell stage embryos and raised for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: ... treated with lambda phosphatase protein after purification at 30°C for 1 hr (New England BioLabs, P0753L), or co-expressed in BL21 cells with RFP-lambda phosphatase ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of N protein was dissolved in 36 μL H218O and 2 μL 10x glycobuffer (NEB). To this solution 2 μL PNGase F was added and the reaction mixture was incubated at 37 °C for 16 h ...
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bioRxiv - Biochemistry 2021Quote: ... with and without the addition of ACE2 (~1:1.25 S-protein trimer:ACE2 molar ratio) (New England Biolabs). Vitrified samples of S-protein constructs with and without ACE2 were prepared by first glow discharging Quantifoil R1.2/1.3 300 mesh holey carbon copper grids for 1 minute using a Pelco easiGlow glow discharge unit (Ted Pella ...
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bioRxiv - Biochemistry 2023Quote: ... Bound protein was detected by western blot using mouse anti-MBP diluted 1:10,000 (New England Biolabs), fluorescent secondary antibodies (Li-Cor Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... 1-cell stage embryos were injected with two guides for each gene with Cas9 protein (NEB, M0646T). Tyrosinase was an injection control to validate the efficacy of the Cas9 protein ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were expressed in BL21 DE3 (New England Biolabs) at 37 °C and 180 rpm after Isipropyl-thiogalactoside (IPTG ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates were additionally dephosphorylated using Lambda protein phosphatase (NEB) following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Then 1.5 ul of 50 μM LbaCas12a protein (NEB) or 1 ul of 63 μM AsCas12a Ultra protein (IDT ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then further purified over amylose resin (NEB) and eluted with elution buffer (20 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... The donor DNA (0.6μM) with Cas9 protein (NEB #M0646T), crRNA and tracrRNA were injected in a 1:1:1:1 molar ratio into mouse zygotes by the UCSD Transgenic and Knockout Mouse Core (See Supplementary Table 4 for crRNA sequence) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were generated using cell lysis buffer (NEB) and brief sonication on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... We digested the proteins with Proteinase K (NEB #P8107S) for 5 min at 50 °C ...
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bioRxiv - Pathology 2021Quote: ... and 0.4 μg of T4 gene protein (NEB, M0300S)] ...
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bioRxiv - Genomics 2021Quote: ... The purified recombinant protein or commercial M.EcoGII (NEB, M0603S) was diluted with coating buffer (0.05 M NaHCO3 buffer ...
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bioRxiv - Microbiology 2021Quote: ... 9 Neuraminidase A (P0722, NEB, 4 units/µg protein). 10 µg glycoprotein ...
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bioRxiv - Microbiology 2020Quote: ... anti-maltose binding protein (MPB) (New England BioLabs #E8032L) and anti-outer membrane protein (OmpA ...
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bioRxiv - Cell Biology 2020Quote: ... against a Color Protein Standard broad range ladder (NEB) in NuPAGE MES SDS buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... A pre-assembled complex of purified Cas9 protein (NEB) and gRNA was injected and the efficiency of Crispr/Cas9-induced mutagenesis in the F0 generation monitored at 24 hpf using a T7 endonuclease assay (Jao et al. ...
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bioRxiv - Microbiology 2020Quote: ... RNA-protein complexes were dephosphorylated with T4 PNK (NEB) for 45 minutes in an Eppendorf Thermomixer at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μM of purified proteins mixed with PEG8000 (NEB) at 10% (w/v ...
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bioRxiv - Biophysics 2019Quote: ... fusion proteins were directly biotinylated with BG-Biotin (NEB), buffer exchanged with a Zeba Desalting Column (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... MBP-MYB6 protein was purified on amylose resin (NEB) and HIS-PUB26 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix ...
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bioRxiv - Synthetic Biology 2020Quote: The protein samples of purified ribosome (New England BioLabs), E ...
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bioRxiv - Genetics 2020Quote: ... 90 nM of SpCas9 protein (#M0386, New England Biolabs), NEB buffer 3.1 ...
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bioRxiv - Neuroscience 2020Quote: ... H1 was transfected with pre-assembled Cas9 protein (NEB) + crRNA and tracrRNA (Synthego) ...
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bioRxiv - Immunology 2021Quote: ... 90 nM of EnGen® Sau Cas9 protein (NEB), NEB buffer 3.1 (NEB ...
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bioRxiv - Genetics 2020Quote: ... MEFs were protein transfected with recombinant RNase H (NEB) using Project Reagent Transfection Kit (Thermo ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were used with lambda protein phosphatase (NEB) treatment according to its protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... using a commercially available Cas9 protein (New England Biolabs). The efficiency of creating deletion after co-injection of the sgRNA pair was determined by PCR on genomic DNA extracted from injected embryos using the following primers:
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bioRxiv - Molecular Biology 2022Quote: ... The eluted protein was applied to Amylose resin (NEB) equilibrated with buffer D (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... 30 μg of Protein G magnetic beads (NEB #S1430) was washed with 250 μl of Immunoprecipitation Reaction Buffer (150 mM NaCl ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions (Lambda protein phosphatase, Biolabs). Western blot analysis was followed with an antibody against GFP (1:1000 ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions (Lambda protein phosphatase, Biolabs). In parallel ...
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bioRxiv - Immunology 2023Quote: ... color prestained protein standard (New England Biolabs GmbH, Germany) was included on each gel ...
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bioRxiv - Bioengineering 2023Quote: ... Tagless protein was expressed in Lemo21(DE3) cells (NEB) in LB (10 g Tryptone ...