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Citations for New England Biolabs :
601 - 650 of 3961 citations for Huntingtin Interacting Protein 1 Related Protein HIP1R Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... thermophilus extracts or PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and the reactions were further incubated at either 50 °C or 37 °C for another 4 h in the case of T ...
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bioRxiv - Molecular Biology 2024Quote: The glycosylation status of the proteins was studied using the PNGase-F and the EndoH (NEB) glycosidases according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... crude extracts were treated with the λ-protein phosphatase (λ-PPase; New England BioLabs, Cat# P0753S) before application on the gel ...
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bioRxiv - Biochemistry 2022Quote: ... purified KRAS protein was mixed with GMP-PNP (molar ratio of 10:1 GMP-PNP:KRAS) and calf intestinal alkaline phosphatase (NEB cat# M0290, 3 units per mg of KRAS). The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified proteins were pre-treated (were indicated) with PNGase F or Endo H (New England Biolabs, USA) according to the manufacturer’s protocol before analysis by Western blotting using Strep-MAb-HRP classic antibody.
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bioRxiv - Molecular Biology 2021Quote: ... 20 μg of protein lysates of each condition was treated with either Endo H (New England Biolabs) or PNGase F for 1 h at 37º C ...
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bioRxiv - Microbiology 2019Quote: In vitro translation-transcription reactions were performed using the Purexpress In Vitro Protein Synthesis system (NEB #E6800). The reaction solution was prepared in the following order ...
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bioRxiv - Plant Biology 2021Quote: ... protein pellets were dissolved in 20 μl of PNGase F denaturation buffer (New England Biolabs, Ipswich, MA) and denatured at 95°C for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were separated by SDS-PAGE and detected by immunoblotting using anti-MBP (New England BioLabs, E8032S), anti-GST (MBL ...
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bioRxiv - Biochemistry 2021Quote: 20 µg protein lysate was incubated with lambda phosphatase according to the manufacturer’s protocol (New England Biolabs). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were loaded directly onto the gel along with a pre-stained protein marker (New England Biolabs), and were run with a constant voltage of 60 V ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of fucose was performed using α1-2,4,5,6 fucosidase O (New England Biolabs, 2 U/μg protein), and α1-3,4 fucosidase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... His-TAD1 fusion proteins were purified according to the manufacturer’s protocol (New England Biolabs, Ipswich, MA, USA). The bait protein GST-WL1 was incubated with GST beads (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Recombinant condensin II holo(WT) at 500 nM was mixed with or without λ protein phosphatase (NEB) at a final concentration of 400 U/µL in 1x NEBuffer for Protein MetalloPhosphatases supplemented with 1 mM MnCl2 and incubated at 30°C for 60 min ...
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bioRxiv - Genetics 2021Quote: Total protein was extracted from dissociated cultured cells by incubating in 1x RIPA buffer (New England Biolabs) with added MS-SAFE Protease and Phosphatase Inhibitor (Sigma ...
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bioRxiv - Physiology 2019Quote: 40 μg of membrane protein were denaturated by adding 2 μl of denaturation buffer (New England Biolabs), for a final volume of 20 μl ...
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bioRxiv - Biophysics 2020Quote: ... proteins are recovered by digesting the DNA with the PtsI restriction enzyme (New England Biolabs, Ipswich, MA), whose cut site was incorporated into all designed oligos.
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bioRxiv - Synthetic Biology 2021Quote: ... Protein expression plasmids were cloned using Gibson Assembly (NEB Gibson Assembly Master Mix, New England Biolabs #E2611) into a pET-28c plasmid backbone and were designed to overexpress recombinant proteins as C-terminus His-tagged fusions ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 μg EBNA2 or 130 μg GST-fused EBNA2 protein were incubate in 1x PK buffer (NEB, 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... The assay was performed using the PURExpress In Vitro Protein Synthesis Kit (E6800S New England Biolabs, Inc.), following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... followed by adapter and motor protein ligation onto the prepared ends using NEB ligase (New England Biolabs). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75-thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Cancer Biology 2021Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75- thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a final concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and incubated with the respective guide at 37°C for 15 min in a 1:1 ratio ...
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bioRxiv - Evolutionary Biology 2022Quote: The TF samples were expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Genetics 2023Quote: ... After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646, NEB, MA, USA), 1nL mixture was micro-injected into single-cell embryos ...
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bioRxiv - Genetics 2022Quote: ... except in the absence of phosphatase Inhibitor and incubated with 400u lambda protein phosphatase (New England Biolabs) at 30°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... 20μg protein was denatured following manufacturer’s protocol followed by PNGase F and O-Glycosidase & Neuraminidase Bundle (NEB) treatment at 37°C for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Biochemistry 2022Quote: ... reactions were performed with 6 μl of PURExpress ΔRF123 in vitro protein synthesis system (New England Biolabs) in the presence of 1x RF3 ...
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bioRxiv - Genomics 2022Quote: ... The TF sample was expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Biophysics 2023Quote: ... concentrated protein was supplemented with 100 U TEV-His7 protease in 1X TEV protease reaction buffer (NEB) and incubated at 30 °C with agitation for 3 hr to cleave the His6-tag ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2023Quote: ... TCA extract in Laemmli buffer was diluted 10-fold in 1X PMP buffer (NEB, Protein Metallo Phosphatases) supplemented with 1 mM Mncl2 ...
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bioRxiv - Molecular Biology 2023Quote: MBP-Nla6 DBD was purified according to instructions in the pMALTM Protein Fusion & Purification System manual (NEB). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
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bioRxiv - Biochemistry 2024Quote: ... The strep tags were removed by incubating the proteins with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... and plasmid pQE70-TstrepSACOL2603myc-2602-2601HA-hisTspA for protein purification were created using HiFi assembly (NEB, E0554S). SACOL2603 ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysis was cleared by centrifugation at 16,100g for 10 min and 0.65 ml supernatant was mixed with 50μl Protein G beads (NEB, #37478S) pre-conjugated to U1-70K antibody (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... Samples were pre-cleared by adding 40 µl Protein A Magnetic Beads (New England Biolabs, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates containing 800μg of protein in a total of 400μl lysis buffer were pre-cleared by incubation with 15μl of Protein A magnetic beads (New England Biolabs) for 1h at 4°C under agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... on ice for 30 min and protein concentration measured by BCA assay (7780, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple) ...
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bioRxiv - Microbiology 2020Quote: ... or CatL-cleaved GPs were incubated with Protein N– glycosidase F (PNGaseF, 250U; New England Biolabs, Ipswich, MA) under reducing conditions for 16 h at 37℃to remove N–linked glycans ...
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bioRxiv - Molecular Biology 2021Quote: ... PARP1 and SET8 GST-fusion and mutant proteins were induced in Escherichia coli ER2566 cells (New England Biolabs
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bioRxiv - Molecular Biology 2022Quote: ... Removal of sialic acids was performed using the α2-3,6,8 neuraminidase (New England Biolabs, 50 U/μg protein). Removal of fucose was performed using α1-2,4,5,6 fucosidase O (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... samples containing the same amount of protein (50 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 µg of total protein (9 µl of lysate) were treated with Endo Hf (New England Biolabs) for 1 h at 37°C ...