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Citations for New England Biolabs :
201 - 250 of 10000+ citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: A 40 µL solution of 25% (v/v) amylose resin (NEB) was co-incubated with 500 nM MBP-RH domain and 4 µM Alexa Fluor 647-labeled RAB7A (life technologies ...
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bioRxiv - Plant Biology 2023Quote: ... 2.5% (v/v) 1 mg/mL bovine serum albumin (NEB #B9200S), 5% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... extracting domain D2 from pDONR221/CD4 using primer pair P4 and performing a Gibson assembly (NEB) with the PCR products ...
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bioRxiv - Developmental Biology 2022Quote: ... The R462M point mutation in the PTP domain was introduced by Q5 site-directed mutagenesis (NEB). pCS2+ constructs were linearized using NotI and transcribed in vitro (mMessage machine SP6 ...
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bioRxiv - Biochemistry 2020Quote: ... Point mutations and domain deletions were generated using QuickChange site-directed mutagenesis with Q5 Polymerase (NEB). All plasmids were verified by DNA sequencing.
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bioRxiv - Immunology 2021Quote: ... Ig kappa or Ig lambda constant domains using the Quick Ligase cloning system (New England BioLabs) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... with 1% (w/v) octylglucoside containing 5 U endoglycosidase H (Endo H) or peptide: N-glycosidase F (PNGase F; both NEB, Frankfurt, Germany) at 37°C for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... libraries were prepared using the NEB Ultra II Library Preparation Kit and NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs). The generation of the libraries followed manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and used this as template to construct DNA libraries using a NEBNext Fast DNA Library Prep Set for Ion Torrent kit (New England Biolabs, Canada). Each sample library contained a unique barcode adapter (NEXTflex DNA Barcodes for Ion Torrent ...
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bioRxiv - Genomics 2021Quote: This material was used for generating sequencing libraries using the Small RNA library prep Set for Illumina (Multiplex compatible) kit (NEB E7330). Typically ...
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bioRxiv - Microbiology 2022Quote: ... LAMP reactions were set up using the NEB WarmStart® LAMP Kit (DNA & RNA) (E1700S, New England Biolabs, Ipswich, Massachusetts, USA) and using the two designed primer sets ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were barcoded using Illumina i5 and i7 adaptors from NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) kit (New England Biolabs) and sequenced using a 150-cycle NextSeq 500/550 High Output Kit v2.5 with 75 forward and 75 reverse reads ...
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bioRxiv - Genomics 2024Quote: ... IVT reactions were set up in 30 μl total volume with the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S) with 2 μl of each NTP ...
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bioRxiv - Genomics 2019Quote: ... The microRNA-Seq library was constructed using NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) following the instructions provided by the manufacturer (NEB, E7300S/L, USA). The library construction was started with 800ng total RNA ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... Human FBP1 mutants were constructed by Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) in the PCDH-V5-FBP1 vectors ...
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bioRxiv - Developmental Biology 2020Quote: ... We tested all primers using 1uL of genomic DNA from H9 human ES cells in a PCR reaction containing 12.5 uL Phusion High Fidelity PCR Master Mix (NEB, M0531L), 1.25 uL 5 uM forward primer ...
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bioRxiv - Plant Biology 2023Quote: ... 5% (v/v) T4 DNA ligase at 400 U/μL (NEB #M0202), 5% (v/v ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2019Quote: ... a master mix was prepared containing 2 µl 10x T4 polynucleotide kinase buffer without ATP (NEB) and 1 µl murine RNase inhibitor per sample and 3 µl were added to each sample together with 2 µl truncated T4 polynucleotide kinase (#M0201 ...
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bioRxiv - Plant Biology 2023Quote: ... the pMIR319C containing pMW#2 reporter constructs were linearized with XhoI (R0146, New England Biolabs, USA) and integrated into the mutant HIS3 locus of the YM4271 host strain (Gift from Ram Yadav ...
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bioRxiv - Physiology 2020Quote: ... 1 nl of CRISPR mixture containing 2,4 μg/μl of gRNA and 0.5 μl Cas9 protein (NEB) was injected in one-cell stage embryos and raised for 24 hours ...
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bioRxiv - Synthetic Biology 2019Quote: The PURExpress in vitro protein synthesis kit (New England BioLabs) was used to transcribe and translate Were-1-Fluc ...
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bioRxiv - Microbiology 2022Quote: Experiments with PURExpress in vitro protein synthesis kit (NEB, E6800) were performed as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: We used PURExpress In Vitro Protein Synthesis Kit (NEB, E6800L) and the reaction was set up with the following manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used to translate the synthetic transcriptome described above ...
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bioRxiv - Microbiology 2023Quote: ... proteins were removed using the Monarch RNA Cleanup Kit (NEB) and 5’-mono-phosphorylated v51_mut_S templates were specifically digested with a terminator 5’-phosphate-dependent exonuclease (Biosearch technologies ...
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bioRxiv - Cell Biology 2023Quote: ... kit and co-injected with Cas9 protein (New England Biolabs) into one-cell stage zebrafish eggs ...
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bioRxiv - Immunology 2022Quote: ... The Multiplex Small RNA Library Prep Set (New England Biolabs) was used to produce small RNA libraries ...
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bioRxiv - Genetics 2023Quote: ... seven independent reactions were set up where the EcoRI (NEB), PsiI (NEB ...
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bioRxiv - Systems Biology 2020Quote: ... The SH3 domain DNA sequence of ABP1 used in the DMS experiments was cloned into pUC19 (NEB) via restriction enzyme cloning with 78bp homology arms surrounding the SH3 DNA (from genomic DNA) ...
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bioRxiv - Cell Biology 2021Quote: ... Domain dissection mutants were subcloned from sfGFP-TAOK2 using restriction enzymes HindIII and MfeI (New England Biolabs). All resultant plasmids were verified by sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... The 3-4FnIII fragment and randomized 5FnIII domain were digested with BsmBI-v2 (New England Biolabs R0739S), purified by PCR cleanup kit (Fisher Scientific FERK0702) ...
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bioRxiv - Neuroscience 2022Quote: ... Domain deletion mutants were subcloned from sfGFP-TAOK1 using restriction enzymes EcoRI and MfeI (New England Biolabs) and assembled using NEBuilder HiFi DNA Assembly-New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: ... The destabilization domain was removed from the plasmid via restriction enzymes BamHI and EcoRI (New England Biolabs) for constitutive expression in the YUMM1.7 cell lines.
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bioRxiv - Neuroscience 2023Quote: ... A synthetic DNA block (IDT) coding for the scrambled E domain was subcloned using Gibson-cloning (NEB). GST-α-syn 96-140 Scr and GST-α-syn 96-110 Scr plasmids were synthesized by GenScript (Piscataway ...
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bioRxiv - Biophysics 2023Quote: ... and the DNA of the SL5-6 domains was then digested using BsaI (New England Biolabs #R0535) followed by Mung Bean Nuclease (New England Biolabs #M0250) ...
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bioRxiv - Genetics 2021Quote: ... using the LTP library preparation kit and employing NEBNext multiplex oligos for Illumina index primer pairs set 1 (New England Biolabs, Ipswich, Massachusetts). Sequencing was performed using a NextSeq 500/550 mid-output v2.5 300 cycle cartridge (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparations were performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina with the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NEB, Ipswich, Massachusetts). Library preparation followed the protocol outlined in Saeidi et al ...
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bioRxiv - Genetics 2022Quote: ... The tRNAs were re-phosphorylated and converted to small RNA sequencing libraries using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs). Size selection of 140-155 bp PCR amplified fragments (corresponding to 19-35 nt tRNA fragments size range ...
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bioRxiv - Microbiology 2023Quote: Libraries were prepared using the NEBNext® DNA Library Prep Reagent Set for Illumina® kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred nanograms of RNA was used to generate sequencing libraries using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-bound proteins were eluted by adding 7.5 μl RNase H (NEB), 2 μl TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S, NEB) for 16h at 30°C.
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared using the NEBNext Immune Sequencing Kit for Human (New England Biolabs) according to the manufacturer’s instructions without modifications ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... and ligated with oligos each containing a Cy5- or BHQ-2 label and NotI sticky ends (NEB) complimentary to the amplified DNA template ...
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bioRxiv - Microbiology 2022Quote: ... samples containing the same amount of protein (50 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... His tagged proteins were eluted with lysis buffer containing 400mM imidazole and bound immediately to amylose resin (NEB) for 1hr ...