Labshake search
Citations for New England Biolabs :
651 - 700 of 2329 citations for Human PRPF31 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... and purified with the Monarch Plasmid Miniprep Kit (T1010, New England Biolabs). Plasmids were digested with restriction enzymes to generate linearized templates for in vitro transcription ...
-
bioRxiv - Microbiology 2022Quote: ... A VSV-G plasmid (16) was digested with KpnI and HpaI (NEB), removing a C-terminal part of G ...
-
bioRxiv - Molecular Biology 2022Quote: ... by combining the original plasmid digested with HindIII (New England Biolabs, #R3104S) and NotI (New England Biolabs ...
-
bioRxiv - Genomics 2022Quote: 100-ng pTXB1 plasmids were firstly linearized by 10U XbaI (NEB, R0145S) at 37°C for 1h and purified by Zymo columns ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Plasmid assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB), followed by transformation into NEB 5-alpha competent E ...
-
bioRxiv - Molecular Biology 2023Quote: ... The plasmids were constructed by NEBuilder HiFi DNA assembly (New England Biolabs) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2022Quote: ... This plasmid was digested with high fidelity (HF) PstI and XhoI (NEB) before insertion of the amplified GPR1 using NEBuilder® HiFI DNA assembly Cloning kit ...
-
bioRxiv - Microbiology 2022Quote: ... Therefore the CIp10 plasmid was digested with HF PstI and XhoI (NEB) before insertion of the amplified Gpr1 and the mTurquoise2 ...
-
bioRxiv - Genetics 2023Quote: ... Linear pUC-GW plasmids and purified SEED cassettes were assembled by NEB assembly following commercial protocols for 1h at 50°C ...
-
bioRxiv - Biochemistry 2023Quote: ... and the resultant plasmids were transformed in E.Coli BL21-DE3 cells (NEB).
-
bioRxiv - Genetics 2023Quote: Plasmid px458 was digested with restriction endonuclease BbsI (New England Biolabs, R0539S), by addition of 1μl enzyme to 1μg plasmid ...
-
bioRxiv - Cell Biology 2023Quote: ... The ligated plasmid was transformed into Stable competent cells (New England Biolabs), and individual clones were picked and sequenced (Laragen ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µg of template plasmid was digested using 1µL AgeI (NEB, R3552S) for 15h in Cutsmart Buffer (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... Backbone plasmids were linearised via PCR followed by treatment with DpnI (NEB) before assembly with fragments using NEBuilder HiFi DNA Assembly (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were assembled via Gibson Assembly (New England BioLabs 2X Master mix) (62 ...
-
bioRxiv - Systems Biology 2023Quote: ... the plasmid backbone was digested and dephosphorylated using BsmBI restriction enzyme (NEB) and Quick CIP (NEB) ...
-
bioRxiv - Cell Biology 2023Quote: ... The obtained yeast plasmid DNA was transformed into competent cells (NEB, C2987H). Yeast strains ...
-
bioRxiv - Synthetic Biology 2023Quote: ... integration plasmids were linearized in a BsaI restriction digestion (BsaI-HFv2, NEB) at 37 °C for 2 h ...
-
bioRxiv - Genetics 2023Quote: ... The BAC was purified purified using the Monarch Plasmid Miniprep Kit (NEB).
-
bioRxiv - Biochemistry 2023Quote: ... Each plasmid was then digested with EcoRI-HF and BamHI-HF (NEB) restriction enzymes ...
-
bioRxiv - Biochemistry 2023Quote: ... Plasmids verified by Sanger sequencing were linearized with AseI (NEB, Catalog # R0526S) and SspI (NEB ...
-
bioRxiv - Biochemistry 2023Quote: ... of the SARS-CoV-2 Positive Control (N gene) plasmid (NEB N2117). Sanger sequencing confirmed successful mutagenesis ...
-
bioRxiv - Developmental Biology 2023Quote: ... from template DNA plasmid digested by NotI or SpeI (New England Biolabs), and were cleaned by RNeasy mini kit (QIAGEN) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Ligated plasmids were transformed into NEB stable cells (New England Biolabs #C3040) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... The pET755 plasmid was used for Q5 polymerase-mediated (New England Biolabs) PCR amplification of the backbones that contain an ampicillin resistance cassette and unc-54 3’utr ...
-
bioRxiv - Immunology 2023Quote: ... The LZRS-CAR-IRES-eGFP plasmids were overnight digested with BamHI (NEB) and purified using Zymoclean Large Fragment DNA Recovery Kit (Zymo ...
-
bioRxiv - Genetics 2023Quote: ... DNA was isolated according to Monarch® Plasmid Miniprep Kit (BioLabs, USA) and the PureYield® Plasmid Midiprep Protocol System (Promega ...
-
bioRxiv - Genetics 2023Quote: Plasmids were constructed using Gibson assembly (New England BioLabs Cat. No. E2611), as follows ...
-
bioRxiv - Plant Biology 2023Quote: ... The plasmid construct was assembled using Gibson cloning58 (E5510S, New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... Plasmid was then constructed using an NEB T4 Ligase protocol (NEB – m0202L) and transformed into competent bacteria (DH5α ...
-
bioRxiv - Systems Biology 2023Quote: ... the plasmid was digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and SacI-HF (New England Biolabs ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The resulting helper plasmids were transformed into DH5α competent cells (NEB, C2987H), yielding four individual helper cell lines (M13cp-dg1 ...
-
bioRxiv - Synthetic Biology 2024Quote: Plasmid libraries are generated using Golden Gate Assembly Kits (NEB, BsaI-HFv2). The reactions undergo a cycling protocol of 30 alternating 5-minute 37°C and 16°C cycles followed by a final 60°C 5-minute hold ...
-
bioRxiv - Microbiology 2023Quote: ... the plasmids were transformed into SHuffle T7 Express (New England Biolabs, USA) containing the plasmid pRARE extracted from Rosetta-gami B(DE3 ...
-
bioRxiv - Biophysics 2023Quote: ... and simultaneous blunt ligation of the plasmid using T4 DNA ligase (NEB). For the N-terminal ybbR-mutant the tag was inserted between E15 and E16 in the β’-subunit ...
-
bioRxiv - Cancer Biology 2023Quote: ... fragments and destination plasmid were digested using the respective restriction enzymes (NEB) and ligation (T4 DNA Ligase ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 DNA fragment plasmids were digested with I-SceI (NEB) to release the overlapping fragments from the backbones ...
-
bioRxiv - Cancer Biology 2023Quote: ... as well as on the website for the original pLentiCRISPRv2 plasmid (Addgene #52961).48,49 Plasmids were transformed into NEB Stable bacteria (New England Biolabs, MA) and colonies were screened for sgRNA inserts by Sanger sequencing of the ligation site ...
-
bioRxiv - Molecular Biology 2024Quote: Plasmid pAM089 was linearized by cleavage with EcoRI-HF (New England Biolabs), separated on a 1x TBE agarose gel and extracted by electroelution ...
-
bioRxiv - Molecular Biology 2024Quote: ... The oligonucleotides (60nM) were hybridized to φX174 ssDNA plasmids (5.3 kb, NEB) (20nM ...
-
bioRxiv - Biochemistry 2024Quote: ... Parental plasmids were digested by adding 5 µL 10x cutsmart buffer (NEB), and 1 µL DpnI (NEB ...
-
bioRxiv - Cell Biology 2024Quote: Plasmids were transformed in BL21 (DE3) competent Escherichia coli (New England Biolabs) or Rosetta (DE3 ...
-
bioRxiv - Genetics 2023Quote: ... which were subsequently inserted into the plasmid backbone using NEBuilder (NEB, E2621). The purifications for PALS-C steps were done with NucleoSpin Gel and PCR Clean-Up kit (Takara ...
-
bioRxiv - Immunology 2021Quote: ... Libraries were prepared using the NEBNext Immune Sequencing Kit for Human (New England Biolabs) according to the manufacturer’s instructions without modifications ...
-
bioRxiv - Genomics 2020Quote: ... the circularized DNA was initially treated with human alkyl adenine DNA glycosylase (hAAG, NEB) to cleave the N-glycosidic bond of etheno-dA bases ...
-
bioRxiv - Cell Biology 2020Quote: ... The ‘601’ fragments were released from the plasmid by digestion with EcoRV (NEB), isolated by incremental PEG precipitation ...
-
bioRxiv - Cell Biology 2020Quote: ... 100 ug plasmid were digested with 100 U Notl-HF and XbaI (NEB) for 7 h at 37 °C ...
-
bioRxiv - Genetics 2021Quote: ... mRNA was generated from the linearized plasmids with the T3 RNA polymerase (NEB). 200 pg of wild-type or mutant pola2 mRNA were injected into embryos from a cross at the 1-cell stage ...
-
bioRxiv - Developmental Biology 2021Quote: ... The resulting plasmid was injected into zebrafish zygotes together with IsceI meganuclease (NEB) as previously described (Pyati et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmid expressing EBFP2-14-3-3 theta was created using HiFi Cloning (NEB) of 14-3-3 theta into pEBFP2-C1 (Addgene plasmid #54665) ...