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Citations for New England Biolabs :
401 - 450 of 2329 citations for Human MMP24 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... plasmid DNA encoding rat HEV was linearized using EcoRI (NEB). Five percent of the reaction was subjected to gel electrophoresis with ethidium bromide staining and visualized with ultraviolet light to verify that linearization had occurred.
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bioRxiv - Biophysics 2023Quote: ... Plasmids were linearized using Nhe I HF (New England Biolabs) and transcribed in vitro (mMessage-mMachine T7 Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... The final donor plasmid was generated through Gibson cloning (NEB) of a synthetic FseI-HA-KRAB-AscI DNA fragment (GenScript ...
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bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids bearing mutations were cut with AseI (NEB, Catalog # R0526S) and SspI (NEB ...
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bioRxiv - Genomics 2023Quote: ... Dual-enSERT-2.1 plasmid was digested with NotI (NEB, R3189) and AgeI (NEB ...
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bioRxiv - Microbiology 2023Quote: ... the plasmids were linearized using EcoRV endonuclease (New England Biolabs). cRNA synthesis was accomplished through in vitro transcription using the HiScribe T7 ARCA mRNA Kit with tailing (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... Gel extraction and plasmid preparation kits were purchased from NEB and Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: We digested the resulting plasmids by NotI-HF® (NEB) and inserted cassettes expressing fusions of antibiotic resistance genes with deltaTK downstream of the lox66 sites by Gibson assembly ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were cloned and propagated in NEB Turbo cells (NEB) — except for pDonorCI derivatives ...
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bioRxiv - Cell Biology 2023Quote: ... and the plasmid was amplified using Q5 DNA polymerase (NEB). The template plasmid was digested with DpnI (NEB) ...
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bioRxiv - Biophysics 2023Quote: Plasmid pUC19 was linearized by using restriction enzymes HindIII (NEB) and XbaI (NEB ...
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bioRxiv - Genetics 2023Quote: ... pAAVsc-JeT-MCOLN1-pA plasmid was digested with EcoRI (NEB) to recover the ITR-flanked AAV genome to be used as standard ...
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bioRxiv - Genomics 2023Quote: ... We digested the plasmid pRS31N with SacII (New England Biolabs), treated the digestion with calf intestinal alkaline phosphatase (CIP ...
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bioRxiv - Synthetic Biology 2024Quote: ... pLK35 plasmids were transformed into NEB Express Iq cells (NEB) which are a bL21 derivative that constitutively expresses the lac repressor ...
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bioRxiv - Genomics 2024Quote: ... plasmids were linearized using BamHI or NotI (New England Biolabs) and in vitro-transcribed using T7 or SP6 polymerase (DIG RNA Labeling Mix ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmids were transformed into DH5a competent cells (New England Biolabs) on LB agar plates with ampicillin ...
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bioRxiv - Microbiology 2024Quote: ... Isolation was performed using Monarch Plasmid Miniprep Kit (NEB, UK).
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bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
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bioRxiv - Cell Biology 2024Quote: ... Before transfection pKD plasmids were digested overnight with EcoRV (NEB). The enzyme was then subjected to heat inactivation for 20 min at 65 °C and then mixed with the pUF1-Cas9 plasmid ...
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bioRxiv - Biophysics 2024Quote: ... The plasmid was linearized with NheI-HF (New England Biolabs) and purified using QIAquick PCR Purification Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg of replicon plasmid was linearised with AscI (NEB), purified by phenol-chloroform extraction ...
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bioRxiv - Biophysics 2020Quote: Recombinant wild-type human histone H1.0 (New England Biolabs, cat. # M2501S) was used for experiments with fluorescently-labeled nucleosomes ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human CHIP was expressed in BL21(DE3) (New England Biolabs) E ...
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bioRxiv - Cell Biology 2024Quote: ... coli-derived recombinant human histone H1 (NEB, Ipswich, MA, USA #M2501), H2A (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Afterwards 2 µg of recombinant human Histone H3.1 (New England BioLabs, M2503), 100 µM S-(5′-adenosyl)-L-methionine chloride dihydrochloride (SAM ...
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bioRxiv - Genomics 2019Quote: ... Human genomic DNA was fragmented by a dsDNA fragmentase (New England Biolabs) followed by end preparation and adapter ligation according to the NEBNext Ultra II DNA Library Prep Method for Illumina (New England Biolabs).
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Biochemistry 2022Quote: ... The coding region of human Cdc20N was cloned by USER® (NEB) into a modified pRSFDuet-1 vector (71341-3 ...
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bioRxiv - Microbiology 2022Quote: ... or PCR amplified from human cDNA using Vent Polymerase (New England Biolabs), then introduced into the luciferase with an intron construct at the EcoRI site ...
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bioRxiv - Genomics 2020Quote: Mouse NIH/3T3 and human Jurkat DNA were from NEB (Ipswich, MA), and XP12 phage DNA was obtained from Dr ...
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bioRxiv - Molecular Biology 2022Quote: ... we used NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7405), NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765 ...
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bioRxiv - Genetics 2023Quote: ... using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB E7405) or the NEBNext Poly(A ...
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bioRxiv - Synthetic Biology 2021Quote: ... The linearized plasmid was then circularized using a KLD reaction (NEB) and transformed into competent cells via electroporation ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5× Q5 buffer and pUC19 plasmid as template (New England Biolabs) in 50 μl ...
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bioRxiv - Genetics 2021Quote: ... 20 μg of mpra:Δorf plasmid was linearized with AsiSI (NEB, R0630S) and 1x CutSmart buffer (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... plasmid pIG1783-Smkin3-gfp was used for Q5 mutagenesis (NEB biolabs). With specific primers (Table S3) ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated plasmid samples were digested by XbaI restriction endonuclease (NEB, Canada). 1 mL in vitro transcription reactions were performed using laboratory purified in-house T7 RNA polymerase and commercial RiboLock RNase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The methylated parent plasmid was degraded with DpnI (New England Biolabs), and newly generated plasmid was transformed into OmniMAX chemically competent E ...
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bioRxiv - Molecular Biology 2021Quote: ... RV-A16 and RV-B14 plasmids were linearized by SacI (NEB) digestion and then in vitro transcribed using HiScribe T7 Transcription Kit (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid pMQ30MRR1-L1191H+Q1197* was digested with AscI (New England BioLabs) and AgeI-HF (New England BioLabs) ...
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bioRxiv - Genomics 2020Quote: ... 59.) using BbsI plasmid digest and Gibson Assembly (New England Biolabs). Spacer sequences were as follows:
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bioRxiv - Immunology 2021Quote: ... Plasmids were transformed in E.coli DH5α competent cells (NEB; Cat,# C2987I). All site-directed changes were confirmed by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: The reporter plasmid was linearized by digesting it with PmeI (NEB) and BstXI (NEB ...
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bioRxiv - Bioengineering 2019Quote: ... Mutated plasmids were transformed into T7 Express cells (New England BioLabs), plated on LB+100 µg/mL ampicillin agar plates and grown overnight at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... The resulting plasmid was then digested with KpnI and NcoI (NEB) for 1 hr at 37°C and ligated with hybridized primers NcoI-KpnI-link-F and NcoI-KpnI-link-R that were similarly digested ...
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bioRxiv - Cancer Biology 2019Quote: ... The pFETCH mutant and wildtype plasmids were SalI (New England BioLabs) digested prior to transfection to linearize the vector.
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bioRxiv - Molecular Biology 2019Quote: ... pGP was modified from a commercially available plasmid pCMV-Gluc (NEB), in which contains a set of cellular 5’-noncoding sequences (41 nts ...
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bioRxiv - Microbiology 2019Quote: ... and Monarch® Plasmid miniprep Kit (New England BioLabs, Ipswitch, MA) was used for purification of plasmid for gene expression and construction of deletion mutant ...
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bioRxiv - Bioengineering 2019Quote: Single stranded plasmid master mix (3x): 3 U/µL Nb.BbvCI (NEB), 36 U/µL Exonuclease III (ExoIII ...