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Citations for New England Biolabs :
1 - 50 of 8884 citations for Human Corticotropin Like Intermediate Lobe Peptide CLIP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... CLIP-tag (NEB), and HaloTag (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB, S9234S]) at final concentrations of 1 μM in 0.3% PBT ...
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bioRxiv - Cell Biology 2020Quote: ... CLIP-Cell TMR star substrate or CLIP-Surface 488 substrate (NEB S9219 and S9232) was reacted with dCas9-CLIP proteins at 37°C for 60min ...
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bioRxiv - Molecular Biology 2023Quote: ... and all constructions and intermediates were generated using a NEBuilder HiFi DNA assembly cloning kit (E5520S, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 8 µM BC-647 (CLIP-Surface 647, NEB) in a base solution of Passive Lysis Buffer was rotated overnight at 4°C to label CLIP-tagged proteins ...
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bioRxiv - Genomics 2024Quote: ... the intermediate library was digested with Esp3I enzyme (NEB R0734S) at 37°C for 6 hr and gel purified ...
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bioRxiv - Immunology 2019Quote: ... cells were labeled with CLIP-Surface 547 (NEB, catalog S9233S) and SNAP-Surface Alexa Fluor 647 (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was collected from each fin clip using the Monarch Genomic DNA Purification kit (New England Biolabs) and amplified using OneTaq 2X Master Mix (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... purified NSD intermediates were digested with 0.4 U/μl AlwnI (NEB) in CutSmart Buffer (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Following circularization of both intermediate constructs using KLD reaction buffer (NEB), a region comprising sgRNA1 was PCR amplified with primers 6 and 7 from pSag1::Cas9-U6::sg1PDE1 and Gibson assembled into Kpn1/XhoI linearised pSag1::Cas9-U6: ...
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bioRxiv - Microbiology 2021Quote: ... Following circularization of both intermediate constructs using KLD reaction buffer (NEB), a region comprising sgRNA1 was PCR amplified with primers 6 and 7 from pSag1::Cas9-U6::sg1CDPK3 and Gibson assembled into Kpn1/XhoI linearised pSag1::Cas9-U6: ...
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bioRxiv - Genetics 2019Quote: ... Both intermediate library and amplified gBlock were digested with BbsI (NEB) and ligated using T4 DNA Ligase (NEB ...
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bioRxiv - Genetics 2019Quote: ... Both intermediate construct and amplified gBlock were digested with BbsI (NEB) and ligated using T4 DNA Ligase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... method linking intermediate wells.53 Concerted pathways were obtained by NEB directly from the reactants to the products ...
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bioRxiv - Genomics 2024Quote: ... This intermediate backbone (pJY127) was then digested with BamHI (NEB R0136S) and NotI (NEB R0189S) ...
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bioRxiv - Molecular Biology 2021Quote: ... were then combined with 10 μM CLIP-Biotin (New England Biolabs) and rotated (end-over-end ...
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bioRxiv - Cell Biology 2021Quote: ... This first intermediate plasmid was linearized via EcoRV (New England Biolabs-NEB) digestion and 2 inserts were added to it by In-Fusion to generate the second intermediate plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... This first intermediate plasmid was linearized via EcoRV (New England Biolabs-NEB) digestion and 2 inserts were added to it by In-Fusion to generate the second intermediate plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... Point variants were introduced into hFibcd1 cDNA while cloned in the intermediate pDONR201 plasmid using the Q5 site-directed mutagenesis kit (NEB #E0554), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Corresponding site-directed mutagenesis was conducted on the intermediate TOPO constructs with specific mutation primers by using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). The mutated E2- or E1-containing fragments were digested from the TOPO constructs through intrinsic viral restriction sites and inserted back into CHIKV through T4 ligation.
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bioRxiv - Genetics 2020Quote: ... This intermediate plasmid product (pLCv2-GFP) was digested with AfeI (NEB, Cat# R0652S) and EcoRI-HF (NEB ...
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bioRxiv - Systems Biology 2023Quote: ... the intermediate library was digested with I-SceI (New England Biolabs, Ipswitch, MA) and NheI-HF (New England Biolabs ...
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bioRxiv - Systems Biology 2023Quote: ... the prey intermediate library was digested with AvrII (New England Biolabs, Ipswitch, MA) and HindIII-HF (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... and inserted to the intermediate plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB). The L-sNTurboID fragment was ligated back to the pCEZ-L backbone using the same restriction sites Pac1 and Hpa1 by NEB T4 ligase.
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bioRxiv - Immunology 2019Quote: ... or SEE together with 1 µM CLIP-Surface 647 (Figure 3C, NEB, catalog S9234S) in RPMI medium for 30 minutes at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... The intermediate library and G-block were column-purified and ligated (T4 DNA ligase, NEB) in three separate reactions for each subpool ...
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bioRxiv - Genomics 2022Quote: ... Original vector backbone and intermediate backbone product were designed for digestion with Esp3I (BsmBI, NEB), inserts were designed for digestion with BsaI (NEB).
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bioRxiv - Systems Biology 2023Quote: ... all wild-type bZIP intermediate plasmids were digested with AvrII (New England Biolabs, Ipswitch, MA) and HindIII-HF (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... the acquired Mlathy INR and INR-like sequences were confirmed by PCR using the Q5 Hot Start High-Fidelity kit (NEB), enzymatic clean-up using ExoSAP-IT™ (Thermo Fisher scientific ...
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bioRxiv - Biochemistry 2021Quote: ... primers were designed using CRY-WT-CLIP as the template by NEBaseChanger (New England Biolabs). The desired DNA was amplified by PCR as above ...
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bioRxiv - Microbiology 2023Quote: All clonings were carried on in a Gibson assembly-like reaction with the NEBuilder® HiFi DNA Assembly kit (NEB, E2621) and into plasmids derived from pMM704 or pMM731 (17) ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Biochemistry 2019Quote: ... we used a commercial assay kit (Ph.D.-7 Phage Display Peptide Library Kit, New England Biolabs) and followed the recommended protocol for “solution phase panning with affinity bead capture” with the following modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of each pFRT-TO-CLIP-UPF1 mutant vector was digested with 1 µL SpeI (NEB) and 1 µL HindIII-HF (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 ng of the intermediate plasmid products were incubated with 10 uM random hexamer primers and 1X phi29 DNA polymerase buffer (NEB) at 95°C for 3 minutes and then cooled to room temperature ...
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bioRxiv - Genomics 2024Quote: ... The purified scaffold insert (2 ng) was ligated with the digested intermediate plasmid library vector (200 ng) using T4 DNA Ligase (NEB) at room temperature for 45 min ...
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bioRxiv - Evolutionary Biology 2020Quote: Phage display experiments were performed using the PhD-12 peptide phage display kit (NEB). All steps involving the pipetting of phage-containing samples was done using filter tips (Rainin) ...
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabsⓇ Inc., MA, USA) was preferred in order to select aggregation inhibitory peptide selection ...
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bioRxiv - Bioengineering 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs□ Inc., MA, USA) was preferred ...
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bioRxiv - Bioengineering 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs□ Inc., MA, USA) was used ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: High throughput sequencing libraries were prepared using RNA isolated from PAR-CLIP samples using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2; cat# E7580, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: A cDNA library of the CLIP samples was prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (#E7300, NEB) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: High throughput sequencing libraries were prepared using RNA isolated from total RNA or PAR-CLIP samples using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2; cat# E7580, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... These fragments were introduced into the previously generated intermediate plasmid (after cutting the plasmid with SpeI [New England Biolabs®, Inc.]) by Gibson assembly to generate the division reporter pUC18-mini-Tn7T-Gm::tetR(BD)-Ptet-mCitrine_mCerulean3-BCD2-P14g.
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... Genotyping of the embryos was performed from the fin clip biopsies with Q5® High-Fidelity DNA Polymerase (New England Biolabs) as stated above ...
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bioRxiv - Microbiology 2020Quote: RNA recovered from the PA-ac4C-seq and PAR-CLIP procedures were used for cDNA library preparation using the NEBNext Small RNA Library Prep Set for Illumina (NEB E7330S), then sequenced using Illumina NextSeq 500 ...
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bioRxiv - Bioengineering 2021Quote: ... Cloning of PCR products and gBlocks in the pKSB- intermediate vector 43 was performed using NEBuilder® HiFi DNA Assembly (New England Biolabs, France) following the manufacturer’s instructions ...