Labshake search
Citations for New England Biolabs :
351 - 400 of 8781 citations for Human CD80 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Illumina Ribo-Zero Plus rRNA Depletion kit followed by library preparation with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... was extracted via the UHMW DNA extraction protocol of the Nanobind CBB Big DNA kit (Circulomics) or via the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs) and quantified using Qubit dsDNA Broad Range kit (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB). Following purification ...
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bioRxiv - Microbiology 2024Quote: ... The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB, China) for RNA and DNA samples ...
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bioRxiv - Cancer Biology 2021Quote: ... Ligations were performed using Quick Ligation Kit (NEB). Reaction mixes were incubated for 5 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... using Quick Ligation Kit (NEB; Cat. No. M2200L). The ligated DNA was PCR amplified with primers containing Illumina paired-end sequencing adaptors ...
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bioRxiv - Developmental Biology 2021Quote: ... we used the EnGen sgRNA synthesis kit (NEB) following the manufacturer’s instructions with the following targeting sequences tnpo1 sgRNA#1 (5’-GGCATGGGGGCCACCTCTTG-3’) ...
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bioRxiv - Developmental Biology 2021Quote: ... NEBNext Ultra II DNA Library Prep Kit (NEB) with 11 cycles of PCR was used to prepare sequencing libraries ...
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bioRxiv - Microbiology 2022Quote: ... treatment and purification using Monarch Cleanup Kit (NEB). RNA copy number was calculated from its molecular weight and absorbance measured by a NanoDrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... PolyA selection/enrichment with Ultra II Kit (NEB) was the method adopted for library preparation.
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bioRxiv - Neuroscience 2021Quote: ... Q5® Site-Directed Mutagenesis Kit (NEB, Inc.) was used to make point mutations to produce five individual EA6-related amino acid substitutions (M128R ...
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bioRxiv - Genomics 2020Quote: ... prepared using the NEBNext kit (New England Biolabs) following the manufacturer’s directions ...
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bioRxiv - Genomics 2020Quote: ... and ribodepleted using the rRNA depletion kit (NEB). The library was then prepared using the NEBNext Ultra II direction Library preparation kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... and the Q5 site-directed mutagenesis kit (NEB) to recreate 4 amino acid changes that were previously found to improve the robustness of tagmentation to lower DNA concentrations (41) ...
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bioRxiv - Neuroscience 2019Quote: ... The NEB Next rRNA Depletion Kit (NEB #E6310X) was used to process 250 ng of total RNA ...
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bioRxiv - Biophysics 2019Quote: ... or pMiniT (NEB PCR cloning kit, Cat. # E1202S) plasmids and sequenced ...
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bioRxiv - Molecular Biology 2020Quote: ... blunting with the Quick Blunting™ Kit (NEB), and circularization with T4 Ligase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... PCRs were performed using Q5 polymerase kit (NEB) in a Perkin- MJ Research PTC-200 Peltier Thermal Cycler or C1000 Touch™ Thermal Cycler (BioRad) ...
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bioRxiv - Molecular Biology 2019Quote: ... the NEB Q5 Site-Directed Mutagenesis Kit (NEB) was used according to manufacturers protocol to introduce silent mutations in the N-cad siRNA1 targeting sequence ...
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bioRxiv - Genetics 2020Quote: ... and purified using a PCR purification kit (NEB).
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bioRxiv - Developmental Biology 2020Quote: ... were ligated with Quick Ligation kit (NEB, M2200S). The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... purified using Monarch Gel Extraction Kit (NEB, USA) and ligated with SmaI-digested and dephosphorylated pUC19 vector using Blunt/TA Ligase Mastermix (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... isolated using the Monarch Plasmid preparation kit (NEB) and verified by sequencing (Source Bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... using an NEBuilder HiFi DNA assembly kit (NEB). These manipulations generated px552-pegRNA-CAG-mCherry.
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bioRxiv - Bioengineering 2021Quote: ... and purified using Monarch RNA cleanup kits (NEB).
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bioRxiv - Bioengineering 2020Quote: A Q5 sitedirected mutagenesis kit (New England Biolabs) was used to insert a hexahistidine tag at the N-terminal of the SP141 gene of the P22GFP expression plasmid via standard PCR protocols ...
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bioRxiv - Biochemistry 2021Quote: ... via NEBuilder HiFi DNA Assembly Cloning Kit (NEB). Vector template was amplified using primers oEcoli-C_48 and 49 ...
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bioRxiv - Biochemistry 2020Quote: The Q5 Site directed mutagenesis kit (NEB, E0554S) was used to make the following mutations All mutant constructs were verified by Sanger sequencing.
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bioRxiv - Biochemistry 2020Quote: ... or the Q5 Site-Directed Mutagenesis Kit (NEB). All subcloned PLCε variants contained an N-terminal His-tag and TEV cleavage site ...
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bioRxiv - Synthetic Biology 2021Quote: ... The NEBNext Ultra RNA Library Prep Kit (NEB) was used for RNA sequencing library preparation ...
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bioRxiv - Microbiology 2020Quote: ... Q5 Site-Directed mutagenesis kit (New England BioLabs) was used to generate point mutations in the WT infectious clone ...
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bioRxiv - Microbiology 2020Quote: ... and libraries were constructed using a kit (NEB Ultra DNA library kit for Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... and a reverse transcription kit (NEB, Ipswich, MA) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... and Monarch plasmid miniprep kit (New England BioLabs) used for plasmid purification ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 μl of SR RT primer (NEB-kit) and 1.75 μl of nuclease-free water to each sample followed by incubation at 90°C for 30 s ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 μl ProtoScript II RT (NEB-kit) was added to each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 μl 5’Ligation Reaction Buffer (NEB-kit) and 1.25 μl of 5’Ligation Enzyme Mix (NEB-kit ...
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bioRxiv - Cancer Biology 2020Quote: ... with Quick ligation kit (NEB; Cat. No. M2200L). The ligated DNA was PCR amplified with primers containing Illumina paired-end sequencing adaptors ...
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bioRxiv - Cell Biology 2021Quote: ... purified with RNA clean up kit (NEB T2050), and cDNA was generated with SuperScript III Reverse Transcriptase (Thermo Scientific 18080044 ...
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bioRxiv - Cancer Biology 2021Quote: ... Ligations were performed using Quick Ligation Kit (NEB). Reaction mixes used were ...
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bioRxiv - Genomics 2022Quote: NEBNext Bacteria rRNA depletion Kit (New England Biolabs) with probes designed for bacteria (included in kit from NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... sgRNA was synthesized using EnGen kit from NEB using the following primer ...
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bioRxiv - Microbiology 2022Quote: ... and gel extraction (Monarch Gel Extraction Kit, NEB), site directed mutagenesis (KLD enzyme blend ...
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bioRxiv - Developmental Biology 2022Quote: ... or NEBNext library Quant kit (New England Biolabs). For each condition ...
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bioRxiv - Biochemistry 2022Quote: ... using the NEBuilder Assembly kit (New England BioLabs). To facilitate the purification process a Strep-tag sequence was included in the reverse primer (primer 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... ProtoScript First Strand cDNA Synthesis Kit (NEB, E6300S) was employed following the producer’s procedure to synthesize the complementary DNA starting with 1 μg total RNA ...
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bioRxiv - Biochemistry 2021Quote: ... and a Monarch PCR & DNA Cleanup kit (NEB) with the following changes ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder® HiFi DNA Assembly Kit (NEB) (pLenti-CMV-CatSpertS:WT-EGFP-Hygro and pLenti-CMV-CatSpertS:Mut-EGFP-Hygro ...
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bioRxiv - Genomics 2020Quote: ... Libraries were prepared using NEBNext Ultra kits (NEB) according to the manufacturer’s standard protocol – this procedure included dA-tailing (Note S1) ...
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bioRxiv - Cell Biology 2020Quote: ... using Gibson Assembly® Cloning Kit (NEB, E5510S) following manufacturer’s instructions ...