Labshake search
Citations for New England Biolabs :
1 - 50 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... we performed PCR2 reactions (10,000gRNAs per 50ul reaction) using 5 ul of the pooled PCR1 product per reaction with Q5 polymerase (NEB). Each biological sample was amplified with PCR2 primers with unique barcodes (Supplementary Table 1) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1X RNAPol Reaction Buffer (New England BioLabs, product number B9012SVIAL), 0.5 mM NTPs (New England BioLabs ...
-
bioRxiv - Immunology 2024Quote: ... The products from these PCR reactions were purified (NEB T1030S). The purified products were sent to Eurofins for Sanger sequencing ...
-
bioRxiv - Genomics 2023Quote: ... The reaction product was transformed into NEBstable competent cells (NEB C3040H), and colonies were inoculated and amplified in LB medium (Gibco 10855001 ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were cloned with the NEBuilder HiFi DNA Assembly reaction (NEB) into the SacI and XbaI sites of pCM009 (30) ...
-
bioRxiv - Biochemistry 2020Quote: ... PCR reaction products were purified with Monarch DNA Clean-up Kits (NEB) with a binding buffer:sample ratio of 2:1 to remove products smaller than 2kb ...
-
bioRxiv - Biochemistry 2024Quote: The reaction products were then mixed with 6× DNA loading dye (NEB) and separated by electrophoresis on a 1.2% agarose gel (Lonza ...
-
bioRxiv - Microbiology 2024Quote: ... The products of the HiFi reaction were transformed into NEB10β competent cells (NEB) and plated on LB agar with appropriate antibiotics ...
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR product was denatured and annealed in an 18 μl reaction (15 μl PCR product, 2 μl NEB Buffer2 (10X), 1 μl nuclease-free H2O ...
-
bioRxiv - Molecular Biology 2023Quote: ... Seven µL of DNA product was circularized in a reaction with 1 µL of 10X T4 DNA Ligase Reaction Buffer (NEB, #B0202A), 1 µL T4 Ligase (NEB ...
-
bioRxiv - Genetics 2022Quote: ... Reaction products were gel purified using the Monarch Gel Purification kit (New England Biolabs) and Sanger sequenced using oligos OFA1204 and OFA1206 to confirm both the sequence of the RPT6 promoter and the synonymous substitutions in the RPT6 ORF ...
-
bioRxiv - Biochemistry 2021Quote: ... reaction products were resuspended in 50 µl relevant manufacturer’s enzyme buffer (1 × Cutsmart (NEB) for nicking with Nt.BspQI or 1 × Buffer R (ThermoFisher ...
-
bioRxiv - Biophysics 2023Quote: ... The resulting reaction product was mixed with 1 μl Exonuclease V (New England Biolabs) and 3 μl of 10 mM ATP in NEB Buffer 4 ...
-
bioRxiv - Cell Biology 2023Quote: ... Product of the isothermal assembly reaction was transformed into NEB Stable cells (C3040H, NEB). Transformed cells were plated on plates of LB media (10 g/L tryptone ...
-
bioRxiv - Microbiology 2024Quote: ... The products of the HiFi reaction were transformed into NEB-5αIq competent cells (NEB) and transformants were selected by growth on plates containing ampicillin ...
-
bioRxiv - Biochemistry 2024Quote: ... Product of the isothermal assembly reaction was transformed into NEB Stable cells (C3040H, NEB). Transformed cells were plated on 1.5% agar plates with LB media (10 g/l tryptone ...
-
bioRxiv - Biochemistry 2021Quote: ... 7 μL of the processing reaction products were treated with 10 units Quick CIP (NEB) in 1X CutSmart buffer (NEB ...
-
bioRxiv - Synthetic Biology 2021Quote: ... all linear polymerase chain reaction (PCR) products (Q5 High-Fidelity DNA Polymerase, New England Biolabs) were extracted by gel extraction and then ligated using NovoRec plus One step PCR Cloning Kit (Novoprotein) ...
-
bioRxiv - Microbiology 2024Quote: ... the product of the digestion reaction was dephosphorylated by the Quick CIP (New England Biolabs). For ligation of the vector and the PCR product ...
-
bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR product was then self-ligated using NEBuilder HiFi DNA Assembly Reaction (NEB) following the standard protocol ...
-
bioRxiv - Genomics 2022Quote: The cDNA reaction products were treated with 20 units of Exonuclease 1 (New England Biolabs) and subsequently purified and concentrated using AMPure XP beads ...
-
bioRxiv - Microbiology 2023Quote: Products of the JVG_BJ1 and JVG_BJ2 reaction were digested prior to assembly using Dpn1 (NEB). The NEBuilder HiFi DNA Assembly kit (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... The PCR products were cloned into the pFK206 vector21 via a Gibson Assembly reaction (NEB). The construct sequence was verified with Sanger sequencing and transferred into plants with the Agrobacteria-mediated floral dip method.
-
bioRxiv - Evolutionary Biology 2024Quote: ... and purified the rest of the digestion product with Monarch Nucleic Acid Purification Kit (NEB). We quantified the amount of purified DNA using the Qubit dsDNA HS Assay (Thermofisher ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... the reaction product was combined with 20 μl digestion mix containing 5 μl ExoI (NEB, M0293S), 5 μl HinFI (NEB ...
-
bioRxiv - Developmental Biology 2023Quote: ... The PCR products were used as templates for transcription reactions using T3 RNA polymerase (Biolabs, M0378S) or T7 RNA polymerase (TOYOBO ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
-
bioRxiv - Bioengineering 2020Quote: ... The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.).
-
bioRxiv - Microbiology 2021Quote: ... Products of PCR amplification were subjected to KLD reaction using Q5 Site-Directed Mutagenesis Kit (NEB, USA) and transformed into NEB 5-alpha Competent E ...
-
bioRxiv - Plant Biology 2021Quote: ... Gel-extracted PCR products were assembled in a cut-ligation reaction via BsaI-HFv2 (New England Biolabs) into vector pRRES208.482 (kindly provided by Alison Huttly ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 µL of the purified product was ligated into a circular plasmid using KLD reaction mix (NEB) incubated for 10 min at room temperature.
-
bioRxiv - Neuroscience 2023Quote: ... Gibson Assembly reaction products were transformed into NEB Turbo high efficiency chemically competent cells (NEB Catalog# C2984I) and plated on LB + Ampicillin plates ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The products were then appropriately mixed in a golden-gate assembly reaction using BsaI-HFv2 (NEB: R3733L) and T4 DNA ligase (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... 10 µl of purified PCR product was used in a 20 µl HiFi assembly reaction (NEB, E2621S). HiFi assembly was performed at 50°C for 1 hour.
-
bioRxiv - Plant Biology 2024Quote: ... The PCR product was then cloned into pHEE401E-mCherry vector using HIFI DNA assembly reaction (NEB, E5520s). Since previous studies described T-DNA alleles of these genes ...
-
bioRxiv - Genomics 2024Quote: ... Subsequent library products were amplified with 10 reaction cycles using NEBNext Q5 Master Mix (New England Biolabs) supplemented with 2 mM MgSO4 ...
-
bioRxiv - Plant Biology 2021Quote: ... Residual primers and nucleic acids were removed from PCR reactions with Exonuclease I (New England BioLabs) and FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were pooled and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2020Quote: ... SSS reaction products (16 μL) were combined with 24 μL HiScribe T7 High Yield RNA Synthesis Kit (NEB) reagent mix containing 4 μL T7 Buffer ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 135 ng purified PCR product from the previous reaction and 25 μl Q5 high fidelity master mix (NEB). The thermocycling parameters were ...
-
bioRxiv - Genomics 2023Quote: ... The circularized product was separated into three different reactions and amplified using rolling circular amplification using Phi29 (NEB) and incubated at 30°C overnight ...
-
bioRxiv - Biophysics 2023Quote: ... The resulting 40 μl of reaction product was mixed with 4 μl of T5 Exonuclease (New England Biolabs) in NEB Buffer 4 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Recovery PCR reactions were carried out with 1 µl of the selection product in Taq DNA polymerase (NEB) reactions with recovery primers (CSR_Rec_SHORT_F1/R1 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The polymerase chain reaction (PCR) products were amplified using Q5 High-Fidelity DNA polymerase (New England Biolabs, MA, USA) with strict accordance to the manufacturer’s protocols ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Polymerase chain reaction (PCR) products and plasmids were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and Sanger sequencing was performed by Eurofins Genomics ...
-
bioRxiv - Molecular Biology 2020Quote: ... SSS reaction products (16 μl) were combined with 24 μl T7 High Yield RNA Synthesis Kit (NEB, Cat #E2040) reagent mix containing 4 μl T7 Buffer ...
-
bioRxiv - Microbiology 2022Quote: ... The products of the HiFi reaction were transformed into NEB-10β (chlamydial transformation) or NEB-5αI (BACTH) competent cells (NEB), plated on appropriate antibiotics ...
-
bioRxiv - Neuroscience 2023Quote: ... The scFv Gibson Assembly reaction products were transformed into NEB Turbo high efficiency chemically competent cells (NEB Catalog# C2984I) and plated on LB + Ampicillin plates ...
-
bioRxiv - Molecular Biology 2023Quote: ... that can base pair to the 3’ nucleotide of the cDNA products as described.75,76 The reaction was stopped by adding proteinase K (1 unit; New England Biolabs) and 25 mM EDTA followed by clean-up with a Monarch PCR & DNA Cleanup Kit (New England Biolabs) ...