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Citations for New England Biolabs :
501 - 550 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: In vitro transcription was carried out on a linear DNA template using the HighScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The unpurified DNA product was then subjected to in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), incubating at 37 °C for 16 h ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We did 12 rounds of high-fidelity PCR amplification (Phusion High-Fidelity DNA Polymerase, NEB, Ipswich, MA) using PCR primers that included one of 12 unique Illumina multiplex read indices (Supplementary Table S2) ...
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bioRxiv - Microbiology 2020Quote: ... was in-vitro transcribed from 1 μg of these templates using the HiScribe T7 High Yield RNA synthesis kit (NEB, E2040S). Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Purified dsDNA templates were used as templates for T7 transcription reactions using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) and bio-16-CTP (Trilink ...
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bioRxiv - Molecular Biology 2021Quote: ... were used as template of the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, #E2050S, New England Biolabs), performed at 37°C for 16 hr ...
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bioRxiv - Biochemistry 2019Quote: ... TR standards for northern blots were in vitro transcribed from PCR products using the HiScribe™ T7 high yield RNA synthesis kit (New England Biolabs). Band intensities were quantified using ImageQuant TL 8.2 (GE Healthcare Life Sciences).
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bioRxiv - Genomics 2019Quote: ... The annealed oligos were used as the templates for in vitro transcription (IVT) using the HiScribe™ T7 High Yield RNA Synthesis Kit (Cat. E2040S, NEB). After IVT ...
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bioRxiv - Biochemistry 2019Quote: RNA was produced by in vitro transcription (IVT) using the HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs, E2040S). DNA templates for the IVT reaction were produced by annealing complementary DNA oligos (IDT ...
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bioRxiv - Bioengineering 2019Quote: ... Unlabeled tracrRNAs were synthesized by IDT or through in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB) using DNA templates containing a T7 promoter binding sequence and full length tracrRNAs ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitated DNA was reverse-crosslinked and used to construct high-throughput sequencing libraries using NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370). DNA libraries were processed on a Illumina HiSeq machine for single-end sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... molecules used in this study were synthesized by in vitro transcription using HiScribe™ T7 Quick High Yield RNA Synthesis Kits (New England Biolabs), or transcribed directly in the in vitro translation (IVT ...
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bioRxiv - Bioengineering 2020Quote: ... The pool of partially double stranded DNA templates was used for in vitro transcription with HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England BioLabs) and the RNA was purified with TRizol (Ambion) ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were chosen to minimize predicted off-target effects with maximum cleavage efficiency and were synthesized by in vitro transcription as previously described26 using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S) or ...
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bioRxiv - Microbiology 2021Quote: Synthesis of RNA in vitro was carried out as previously described using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050) [35 ...
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bioRxiv - Microbiology 2021Quote: ... and fifteen repeats of the FG-repeats were amplified from genomic DNA using primers (Table S2) with Phusion High-Fidelity PCR Kit (NEB - E0553S). The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020) ...
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bioRxiv - Biochemistry 2022Quote: ... UPF2L (120-1227) were produced by PCR amplification (primers listed in Supplementary Table S1) using the Phusion High-Fidelity PCR kit (NEB #E0553S) prior to restriction digest and ligation into pPROEX-HTB (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Immunology 2020Quote: CAR-encoding mRNA was transcribed from linearized plasmids encoding anti-human CD19 CAR61 and anti-mouse CD19 CAR71 using T7 RNA polymerase (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs). The mRNAs were transcribed to contain the 5′ UTR derived from the human a-globin 5′ leader RNA ...
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bioRxiv - Microbiology 2021Quote: ... 10 ul of this was then used as an input for T7 polymerase mediated In vitro Transcription (IVT) using the NEB HiScribe T7 High Yield RNA Synthesis Kit (NEB, # E2040S). Briefly ...
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bioRxiv - Bioengineering 2022Quote: RNA fragments of AR-V7 and YAP1 sequences were synthesized from DNA gblocks (Integrated DNA Technologies) utilising the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions including the DNase step ...
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bioRxiv - Genetics 2019Quote: ... bps) was then column purified and transcribed using the T7 Quick High Yield RNA Synthesis kit (New England BioLabs, Ipswich, MA) and purified using RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Immunology 2019Quote: ... T7 promoter sequences were added by PCR and the PCR product was used as template to generate sgRNAs by in vitro transcription using the HiScribe T7 quick high yield RNA synthesis kit (NEB E2050S) and purified by an RNeasy mini kit (Qiagen 74104) ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was linearized with BamHI digestion and further recovered as a template for in vitro transcription via HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). RNA was isolated by 15% denaturing PAGE and dissolved in stocking buffer (300 mM NaAc pH 5.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... 150 ng of double stranded gBlock® template or 2 µg of plasmid template was transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs®). At the end of the reaction ...
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bioRxiv - Biochemistry 2020Quote: GQES7-a and GQES7-b were synthesized in vitro by transcription (HiScribe™ T7 High Yield RNA Synthesis Kit; New England Biolabs). GQes3 and mutes3 ...
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bioRxiv - Molecular Biology 2019Quote: The cDNA encoding full-length spliced DIGIT was in vitro transcribed using the HighScribe T7 High Yield RNA synthesis kit (NEB, E2040s). Cyanine 3-UTP (Enzo Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... and the corresponding RNAs were synthesized via in vitro transcription using the HiScribeTM T7 Quick High Yield RNA Synthesis Kit (New England Biolabs Inc.) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... We then performed in vitro transcription from these amplicons using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), and purified the resulting RNA using a GeneJET RNA Purification Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... plus 280 nM each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Genomics 2021Quote: ... were used as template of the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit; #E2050S, New England Biolabs), performed at 37°C for 16h ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... Transcription templates were amplified from the plasmids by PCR and and transcribed in vitro with the HiScribe T7 High Yield RNA synthesis kit (New England Biolabs Inc.). To obtain sufficient amounts of mRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... IRES-Puro and an FRT-flanked PGK-hygromycin selection cassette to generate the targeting vector using the NEBuilder High-Fidelity DNA Assembly Cloning Kit (NEB, E5520). Twenty-one bp downstream of the start codon ...
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bioRxiv - Developmental Biology 2021Quote: ... The gRNA templates were amplified by PCR with scaffold reverse primer then were transcribed with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) [22] ...
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bioRxiv - Genetics 2021Quote: ... and 1 μg of purified PCR product was used as template for in vitro transcription using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S) as described by the manufacturer.
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... The guide region was then amplified by PCR and paired guide RNAs synthesised by in vitro transcription (T7 Quick High Yield RNA Synthesis kit, NEB, #E2050S). Single stranded DNA oligonucleotides (WT oligo ...
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bioRxiv - Cancer Biology 2022Quote: In vitro transcription of LINC00313 or firefly luciferase (F-luc) mRNA was performed using the HiScribe™ T7 High Yield RNA Synthesis kit (New England Biolabs), according to the instructions by the manufacturer ...
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bioRxiv - Developmental Biology 2022Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Microbiology 2022Quote: crRNAs were synthesized for in vitro cleavage experiments using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs; NEB). Templates for in vitro transcription were generated by hybridizing a ssDNA oligo with the T7 promoter to another oligo with the complementary T7 promoter sequence fused to the LbCas12a repeat and spacer in annealing buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 µl PCR amplified product was used for the in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), incubating at 37 °C for 18 hours in the thermocycler ...
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bioRxiv - Microbiology 2023Quote: ... Substrates were transcribed in vitro from purified PCR products using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Developmental Biology 2022Quote: 50mg of muscle tissue were obtained from a euthanized adult female sugar glider and high-molecular weight DNA was extracted using the Qiagen MagAttract HMW kit (NEB 67563). DNA was quantified using the Qubit DNA HS kit (Thermofisher Q32851 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The purified product was used as template for dsRNA synthesis using HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) with subsequent purification with MEGAclear™ Transcription Clean-Up Kit (Ambion) ...
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bioRxiv - Developmental Biology 2023Quote: ... using Custom Nextera PCR primer and the NEBNext High-Fidelity 2x PCR Master Mix (Nextera DNA Library Preparation Kit (New England BioLabs, E7530L) and purified with the Qiagen MinElute Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... All mRNAs were transcribed at 30°C for 2 hrs using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) and were co-transcriptionally capped (8:1 cap analog to GTP for ∼90% capping efficiency ...
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bioRxiv - Biochemistry 2023Quote: ... A 2 µL aliquot of the annealed oligonucleotides was used as template for in vitro RNA transcription by HiScribe T7 quick high yield RNA Synthesis kit (New England Biolabs, MA). The transcribed RNA was purified using RNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Physiology 2024Quote: ... High-quality RNA samples were used to construct sequencing libraries with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), and the libraries were sequenced using Illumina HiSeq X ten/NovaSeq 6000 system (2 × 150 bp read length ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 µl of the PCR-amplified product was employed for in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), with incubation at 37 °C for 18 hours in a thermocycler ...