Labshake search
Citations for New England Biolabs :
101 - 150 of 4340 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Nsp1–40S complex was then affinity-purified using amylose resin (NEB), followed by size-exclusion chromatography ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; New England Biolabs, S1402S) for 10-20 minutes in a 37°C water bath ...
-
bioRxiv - Cell Biology 2021Quote: ... 20 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA, USA), 40 U/ml RNasin (Promega ...
-
bioRxiv - Immunology 2021Quote: ... 20 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA, USA), 40 U/ml RNasin (Promega ...
-
bioRxiv - Immunology 2021Quote: ... 20 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA, USA), 40 U/ml RNasin (Promega ...
-
bioRxiv - Developmental Biology 2021Quote: ... Digestions were stopped with 20 mM ribonucleoside vanadyl complex (NEB: S1402S). The samples were then loaded onto 20%-50% sucrose gradients and ultracentrifuged in a SW41 Ti swinging-bucket rotor (Beckman 331362 ...
-
bioRxiv - Cell Biology 2020Quote: ... and 10 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA), 10 minutes in 4% paraformaldehyde (Ted Pella ...
-
bioRxiv - Genomics 2021Quote: ... 10% Dextran sulfate Sigma D8906; 0.02% BSA Ambion AM2616; 1 mg/ml E.coli tRNA Sigma R1753; 2 mM Vanadyl-ribonucleoside complex NEB S1402S; 2XSSC) containing diluted probes and incubated over night at 30°C ...
-
bioRxiv - Biophysics 2019Quote: WT EcSSB and T7 DNA polymerase were purchased (NEB). The plasmid encoding WT EcSSB pEAW134 was a gift from Dr ...
-
bioRxiv - Biophysics 2022Quote: ... 2018) and a C-terminal 6x His tag and cloned into the pET21B vector by Hi-Fi DNA assembly (NEB).
-
bioRxiv - Cell Biology 2022Quote: ... pET17b-Kif5b(1-560)-GFP-His was transformed into BL-21[DE3]RIPL cells (New England Biolabs) until an optical density at 600 nm of 0.6-0.8 and expression was induced with 0.5 mM isopropyl-β-d-thiogalactoside (IPTG ...
-
bioRxiv - Biochemistry 2022Quote: Cysteine mutations were introduced into a pENTR1A FLAG (FT) WT F3 or pENTR1A 3xFT WT F3 plasmid using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, USA). pENTR1A constructs were shuttled into the pcDNA DEST40 vector (Life Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... TSF-ATG101:ATG13 (1-197) complex (final 3 μM) were incubated with 30 μl Amylose resin (New England Biolabs, Ipswich, MA) at 4 °C overnight in the ITC buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
-
bioRxiv - Genomics 2023Quote: ... 0.5µL of 44µM crRNA:tracrRNA complex and 0.9µL 20µM EnGen SpyCas9 NLS (NEB) per electroporation were mixed and incubated at RT for 10-20 mins ...
-
bioRxiv - Developmental Biology 2019Quote: ... HEK293 DNA was extracted and amplified by Q5 High-Fidelity PCR (NEB, M0494S) using primers encompassing the sgRNA recognition site ...
-
bioRxiv - Biochemistry 2022Quote: ... with recombinant proteins (200-500 ng) and yeast purified CK2 or recombinant human CK2 (NEB, P6010S). Proteins were resolved by SDS-PAGE and the gels were exposed to autoradiography or stained with Coomassie blue.
-
bioRxiv - Biochemistry 2020Quote: 1 μg of human recombinant histone H4 (BioLabs, Cat# M2504S) was incubated with 50 μM n-butyryl- or isobutyryl-CoA and 0.2 μM of HAT1 at 30 °C for 1 hour ...
-
bioRxiv - Cancer Biology 2020Quote: ... RNAsin was replaced with 10 mM ribonucleoside vanadyl complex (New England Biolabs S1402S) was added to the ground tissue ...
-
bioRxiv - Cell Biology 2021Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; S1402S; New England Biolabs; Ipswich, MA) for 10–20 min in a 37°C water bath ...
-
bioRxiv - Molecular Biology 2021Quote: A purified CK2 complex was purchased (#P6010S, New England BioLabs, Beverly, MA, USA). GST-mNRK565-868 ...
-
bioRxiv - Microbiology 2020Quote: ... The trimer complex was then partially deglycosylated using endoH digestion (New England Biolabs) (23 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The Cas9/gRNA complex was generated as described by the manufacturer’s protocol (NEB). For pks1 deletion ...
-
bioRxiv - Molecular Biology 2022Quote: ... all the buffers above were supplemented with 10 mM ribonucleoside-vanadyl complex (NEB) to inhibit ribonucleases ...
-
bioRxiv - Biochemistry 2022Quote: ... The enzyme complex was then further purified by incubation with chitin beads (NEB), to remove traces of TEV and fully active kinase or phosphatase ...
-
bioRxiv - Cell Biology 2024Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; S1402S; New England Biolabs; Ipswich, MA) for 10–20 min in a 37°C water bath ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... was cut with Hind III and Bam HI (NEB) for 1h at 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... and His-MBP-ZFC3H1 onto an Amylose resin (NEB). After extensive washing ...
-
bioRxiv - Immunology 2021Quote: ... were assembled using Hi-Fi DNA Assembly Mix (NEB). For the SFFV-Cas9 vector ...
-
bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10µg/ml) and apyrase (25mU/ml, NEB); the suspension was incubated for 1h at room temperature ...
-
bioRxiv - Synthetic Biology 2020Quote: ... or Hi-Fi assembly from New England BioLabs (NEB) and other basic molecular cloning approaches ...
-
bioRxiv - Genomics 2021Quote: ... was then inserted by Hi-Fi assembly (NEB, E2621) using 0.025 pmols of vector and 0.05 pmols of the GFP amplicon ...
-
bioRxiv - Cell Biology 2021Quote: ... using NEB Hi-Fi assembly mix (New England Biolabs). A mixture of the two guide plasmids (each at 100ng/µl ...
-
bioRxiv - Developmental Biology 2023Quote: ... or Q5 Hi-Fidelity DNA Polymerase (New England Biolabs) was used ...
-
bioRxiv - Biochemistry 2024Quote: ... We expressed the His-SUGCT in Lemo cells (NEB), inoculating 4L of TB with 4 mL overnight culture ...
-
bioRxiv - Microbiology 2024Quote: ... The HIV-1AC-1 mutations were introduced into WT and N74D pNLdE-luc using the Gibson Assembly Cloning kit (New England Biolabs) and primers shown in Table S1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were resuspended in 1 mL spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 µM beta-mercaptoethanol), and 1.2–5 µL 100T zymolyase (US Biological Z1005 ...
-
bioRxiv - Microbiology 2021Quote: ... WT and CpG_1.0 or CpG_max with SalI-HF (New England Biolabs) and WT and UpA_max with HaeIII (Invitrogen ...
-
bioRxiv - Developmental Biology 2021Quote: ... pFastBac dual vector containing the sequence for the N-terminally 6xHis-tagged human Naa15 and truncated human Naa10 (residues 1-160) sequences was digested using KpnI-HF (NEB) and XmaI (NEB ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 10 min with 2 mM Ribonucleoside-Vanadyl Complex RVC (New England Biolabs, S1402S) at room temperature and then permeabilized for 5 min on ice in 0.5% Triton-X with 2mM RVC ...
-
bioRxiv - Molecular Biology 2021Quote: The DNA-complex-coated beads were incubated with the end-repair enzyme mixture (NEB) at 20 °C for 30 min to repair the DNA ends and then with Klenow exo- (NEB ...
-
bioRxiv - Pathology 2023Quote: ... was ligated to the RNA-cDNA hybrid complex using T4 DNA Ligase (NEB M0202L) and subsequently purified with the Agencourt RNAClean XP beads (Beckman-Coulter ...
-
bioRxiv - Genomics 2023Quote: ... as described before. Cas9-gRNA complex (6.6 uM S. pyogenes Cas9 Nuclease (NEB, M0386T), 20 uM gRNA ...
-
bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
-
bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
-
bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
-
bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
-
bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
-
bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
-
bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...