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Citations for New England Biolabs :
401 - 450 of 1480 citations for Guanine Nucleotide Binding Protein G Protein Alpha Activating Activity Polypeptide O GNAO1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Recombinant RfxCas13d proteins were expressed in E.coli NiCo21(DE3) (NEB C2529). Cells were grown in 1L of lysogeny (Luria-Bertrani ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µl of lambda protein phosphatase (New England BioLabs, Ipswich, MA). Each mixture was incubated at 30 °C for either 1 ...
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bioRxiv - Plant Biology 2020Quote: ... the purified proteins (0.125 mg) or RNase If (1.25 U; NEB) were incubated at 25°C for 60 minutes with a fluorescent-labeled RNA substrate (5’-fluorescein isothiocyanate (FITC)-CUUUUAG(A20) ...
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bioRxiv - Molecular Biology 2022Quote: ... and α1-3,4 fucosidase (New England Biolabs, 4 U/μg protein). All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7719).
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then digested with LysC endoproteinase (0.25 µg; NEB) for 4 hours at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... MBP fusion proteins were purified on amylose beads (New England Biolabs) in column buffer (20mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was first bound to amylose resin (New England Biolabs). Afterwards ...
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bioRxiv - Cancer Biology 2019Quote: ... After cooling 5 µl Protein Deglycosylation Mix II (New England Biolabs) was mixed in gently ...
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bioRxiv - Bioengineering 2020Quote: IVPS was performed using PURExpress In Vitro Protein Synthesis Kit (NEB) with supplements (16 U RNase inhibitor (NEB) ...
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bioRxiv - Pathology 2021Quote: ... Soluble protein extracts were purified using amylose resin (New England Biolabs) and/or Ni-NTA agarose beads (Life Technologies).
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bioRxiv - Biochemistry 2021Quote: ... the purified proteins were incubated with excess streptavidin (New England Biolabs) for 10 minutes before analysis via gel-shift using SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein (2.12 ng) (New England BioLabs Inc. Ipswich, MA, USA,) and gRNA (133 pg ...
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bioRxiv - Developmental Biology 2021Quote: ... Purified sgRNA and Cas9-NLS protein (New England Biolabs® Inc.) were diluted to 300ng/ul.
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then added to protein A magnetic beads (NEB, S1425S) and rotated at 4°C for 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were purified using amylose resin column (New England Biolabs). Fractions were eluted and checked by silver staining ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were induced in SHuffle Express strains (New England Biolabs) upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: Protein extract samples (5µl) were subjected to EndoHf (New England Biolabs) treatment according to manufactures instructions ...
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bioRxiv - Genomics 2022Quote: ... EnGen Spy Cas9 NLS protein (20 μM) was purchased from NEB. The injection mixture consisted of 600 ng/μl Cas9 protein ...
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bioRxiv - Genetics 2022Quote: ... 0.8pg of 3xNLS-SpCas9 protein and 1mU of I-SceI (NEB) was injected per embryo at early 1-cell stage ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Molecular Biology 2022Quote: ... 2) 7 µL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per slide was added for deglycosylation and incubated overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... for GST fusion proteins or amylose resin (New England Biolabs #E8021S) for MBP fusion proteins for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... then proteins were eluted in 30μL 3x Sample Loading Buffer (NEB) at room temperature for 10 minutes.
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bioRxiv - Cell Biology 2023Quote: ... A mixture containing 600 ng/uL of Cas9 protein (NEB: M0386) and sgRNA complex at a 1:1 ratio was generated with a total volume of 3 uL ...
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bioRxiv - Neuroscience 2023Quote: ... Select samples were treated on-bead with lambda protein phosphatase (NEB) in the manufacturer’s PMP buffer to remove phosphorylation ...
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bioRxiv - Microbiology 2023Quote: ... MBP-fused proteins were purified from supernatants using amylose resin (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and sizes were estimated using Blue Protein Standard (New England Biolabs). N-terminally 6×His-tagged fusions of human and Drosophila SARM1 TIR have an expected molecular weight of 19.2 kDa.
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bioRxiv - Biochemistry 2023Quote: ... MBP fusion proteins were purified on amylose beads (New England Biolabs) in column buffer (20 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) and 4000 U lambda protein phosphatase (New England Biolabs) added per 1 mg digested lysate ...
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bioRxiv - Genomics 2023Quote: ... and 200 ng/µl Cas 9 protein (New England BioLabs, M0386T). Injected individuals were screened (see below genotyping procedure ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μl of Lambda protein phosphatase (P0753L, New England BioLabs) were incubated with the proteins at 30°C for 30 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... Protein-containing fractions were pooled and incubated with Chitin Resin (NEB) for 30 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... SNAP-tagged proteins were labelled with Cell-SiR647 (NEB, Frankfurt, Germany). Labeling was done at RT for 1hr ...
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bioRxiv - Microbiology 2023Quote: The PURExpress ΔRibosome In Vitro Protein Synthesis Kit (New England Biolabs) was used for in vitro translation assays ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µL of Colour Prestained Protein Standard (New England Biolabs #P7719S) was included on each gel ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were mixed with Cas9 protein (M0646M NEB, or B25641 invitrogen) and coinjected into one-cell stage embryos using a microinjector (Eppendorf) ...
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bioRxiv - Biochemistry 2024Quote: ... Dephosphorylation reactions were performed with Lambda Protein Phosphatase (Lambda PP) (NEB). 1,000 units of Lambda PP were utilized per µmol of Ycf1 protein in a 500 µL reaction ...
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bioRxiv - Biochemistry 2024Quote: Purified MBP-fusion proteins were treated with TEV-protease (NEB, P8112S) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was expressed using the strain T7express lysY (NEB, C3010I). Expression was induced with 0.4 mM IPTG at OD600 ∼0.6 at 16°C ...
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bioRxiv - Cell Biology 2024Quote: ... were combined with protein kinase buffer (PK buffer, New England Biolabs: 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... each protein construct was transformed in either BL21 DE3 pLysS (NEB) harbouring a truncated OGT version with an intact catalytic core (OGTSW ...
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bioRxiv - Cancer Biology 2021Quote: ... and capped and 2’-O-methylated with Vaccinia Capping System (NEB Biolabs). LNA oligos were transfected into unlabeled 4T1 cells using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and capped and 2’-O-methylated with Vaccinia Capping System (NEB Biolabs). LNA oligos were transfected into unlabeled 4T1 cells using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for 1 hr at 72° ...
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bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for one hour at 72°C ...
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bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for one hour at 72°C ...
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bioRxiv - Biophysics 2022Quote: ... Nucleotide sequence corresponding to residues 1-332 of Rv3881c was amplified using Phusion polymerase (NEB) and cloned into pET28a between NdeI and HindIII restriction sites ...
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bioRxiv - Cell Biology 2022Quote: SLC25A46 was amplified through PCR with Taq-polymerase with 7-deaza GTP/nucleotide mix (NEB) using cDNA from control fibroblasts as a template and cloned into Gateway modified pBABE-Puro using Gateway Cloning Technology (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μM Rad51 and 10 μM (nucleotide) φX174 circular single-stranded DNA (ccsDNA) (N3023L, NEB) were mixed in reaction buffer (25 mM Tris-HCl [pH 7.5] ...