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Citations for New England Biolabs :
51 - 100 of 952 citations for Goat Anti Mouse IgG Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and 1 U of shrimp alkaline phosphatase (NEB, Cat# M0371S) in a 50 μL buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were treated with Shrimp Alkaline Phosphatase (SAP; from NEB). In short ...
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bioRxiv - Cell Biology 2023Quote: ... dephosphorylated with Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs; #M0371) and gel purified with the QIAquick PCR & Gel Cleanup Kit (Qiagen ...
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bioRxiv - Genetics 2022Quote: ... Digested backbones were treated with shrimp alkaline phosphatase (NEB M0371S) for 2 hours at 37 °C prior to gel extraction ...
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bioRxiv - Neuroscience 2023Quote: ... the purified RNA was dephosphorylated with shrimp alkaline phosphatase (NEB) supplemented with 100 mM CaCl2 ...
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bioRxiv - Cancer Biology 2024Quote: ... and Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs, cat. M0371S) after AR-DBD_ERT2 construct NheI fragmentation to dephosphorylate ...
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bioRxiv - Genomics 2020Quote: ... RNA samples were treated with 10U shrimp alkaline phosphatase (NEB, M0371S) at 37°C for 1 hr and then labeled with 0.5μl of gamma 32P-ATP (PerkinElmer ...
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bioRxiv - Genomics 2019Quote: ... and the linearized vector was simultaneously dephosphorylated with alkaline phosphatase (NEB). Digestion products were purified with the Gel Extraction Kit (Thermofisher ...
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bioRxiv - Biochemistry 2022Quote: ... The digested pET28a was further reacted with shrimp alkaline phosphatase (NEB) and allowed to react overnight at 16 °C ...
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bioRxiv - Microbiology 2020Quote: ... 0.434 units of calf intestinal alkaline phosphatase (Quick CIP, NEB, MO525S) per μg of His-SUMO-CwlMMtb was added to the reaction mixture and incubated for 1 hour at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... We used calf intestinal alkaline phosphatase (M0290, New England Biolabs, MA) to dephosphorylate the 5’ and 3’ ends of the linearized plasmid to prevent self-ligation of linearized plasmid [90] ...
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bioRxiv - Genomics 2019Quote: ... 10µg total RNA was treated with Calf intestinal alkaline phosphatase (NEB) to remove 5′P from fragmented and non-capped molecules ...
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bioRxiv - Immunology 2020Quote: ... and dephosphorylated using recombinant shrimp alkaline phosphatase (New England BioLabs M0371L). Annealed and phosphorylated oligonucleotides were cloned into linearized and dephosphorylated BPK1520_puroR using T4 DNA Ligase (New England BioLabs M0202L ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was treated with shrimp alkaline phosphatase (New England Biolabs) and purified with MinElute kit (Qiagen) ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... After dephosphorylation with alkaline phosphatase from calf intestine (New England Biolabs), tRNAs were ligated to 100 pmol of 5’ adenylated and 3’ end-blocked DNA oligo (3’ linker ...
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bioRxiv - Molecular Biology 2021Quote: ... and dephosphorylated with 1 U calf intestinal alkaline phosphatase (NEB, M0290), followed by decapping and circularization with RNA 5’ Pyrophosphohydrolase (NEB ...
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bioRxiv - Neuroscience 2023Quote: Dephosphorylation was performed on the resin with shrimp alkaline phosphatase (NEB) supplemented with 100 mM CaCl2 ...
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bioRxiv - Systems Biology 2023Quote: ... dephosphorylated with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA), gel-purified with the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Systems Biology 2023Quote: ... alongside 5 U of quick alkaline phosphatase (calf intestinal, NEB #M0525S), all in a 30 µl rCutSmart buffer (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mU of phosphodiesterase II from Sigma (# P9041-10 UN) and 2 U of alkaline phosphatase from Biolabs (# M0290) were added and the mixture was incubated at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were dephosphorylated with Alkaline Phosphatase (20 U; M0290S, NEB, Ipswich, MA) and T4 PNK (10 U ...
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bioRxiv - Microbiology 2021Quote: ... and subsequently 5’-dephosphorylated with Calf intestinal alkaline phosphatase (New England Biolabs). CviAII cuts on a sequence motif ‘CATG’ which is highly frequent on bacterial genomes ...
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bioRxiv - Biochemistry 2021Quote: ... and 1.3 units of quick calf intestinal alkaline phosphatase (New England Biolabs) in buffer containing Tris-HCl (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of calf intestinal alkaline phosphatase (CIP, NEB, 2Units). The mixture was incubated at 37°C for 1 hour to convert nucleotide 5’-monophosphates to their respective nucleosides ...
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bioRxiv - Microbiology 2019Quote: ... The digested pcDNA3.1-NefSF2V5-VC was treated with shrimp alkaline phosphatase (NEB), then ligated with the PCR products overnight at 16°C and transformed into TOP10 competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... NH4HCO3 (100 mM) and 5 units of alkaline phosphatase (CIP) (NEB, #M0525S) were added and the sample incubated for 2 hours (or 20 min ...
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bioRxiv - Immunology 2022Quote: ... and subsequently incubated with 4 U of alkaline phosphatase (New England Biolabs) at 37°C for 30 min to hydrolyze unreacted NTPs ...
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bioRxiv - Microbiology 2022Quote: ... The linear backbone was then dephosphorylated using shrimp alkaline phosphatase (rSAP, NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 1.5 units of alkaline phosphatase was added to the sample (NEB M0371L) and incubated at 25°C for 1h ...
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bioRxiv - Cell Biology 2023Quote: ... mCherryC1 empty vector was also digested along with shrimp alkaline phosphatase (NEB) treatment ...
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bioRxiv - Plant Biology 2023Quote: ... the purified vector was dephosphorylated by CIAP (Calf intestinal alkaline phosphatase, NEB). The insert and the vector were ligated at a 5:1 molar ratio ...
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bioRxiv - Bioengineering 2023Quote: ... and subsequently treated with calf intestinal alkaline phosphatase (New England Biolabs, M0525L). The digested vector was separated by gel electrophoresis (1% agarose) ...
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bioRxiv - Systems Biology 2023Quote: ... and treated with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA). A fragment containing the CYC terminator ...
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bioRxiv - Genomics 2023Quote: ... treated the digestion with calf intestinal alkaline phosphatase (CIP, New England Biolabs) and purified the digested plasmid using SPRI beads ...
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bioRxiv - Neuroscience 2023Quote: ... The recipient vector was dephosphorylated with shrimp alkaline phosphatase (rSAP NEB #M0371S) during the digest ...
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DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... The reaction was enzymatically cleaned with Exonuclease I and Shrimp Alkaline Phosphatase (NEB) by incubating the sample and enzymes for 30 minutes at 37°C.
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bioRxiv - Cell Biology 2022Quote: ... Following in vitro dephosphorylation using calf intestinal alkaline phosphatase (#M0290, New England Biolabs) the construct was purified with a Qiaquick PCR purification Kit (#28104 ...
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: ... 100pmol triphosphorylated RNA was first dephosphorylated using shrimp alkaline phosphatase (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... DNA was next dephosphorylated with 2 μl calf intestinal alkaline phosphatase (NEB M0290S) and incubated at 50°C for 60 min and immediately purified by 2x AmpureXP beads ...
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bioRxiv - Genetics 2019Quote: ... the vector was treated with 2 μl of calf intestinal alkaline phosphatase (NEB) at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2019Quote: Dephosphorylation assays were performed with Calf Intestinal Alkaline Phosphatase (CIP; New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... The linearized PCR product was dephosphorylated using Shrimp Alkaline phosphatase (New England Biolabs) per the manufacturer’s instructions and then ligated with tetL to generate the deletion construct PB408 ...
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bioRxiv - Genetics 2022Quote: ... The backbone was dephosphorylated using Calf Intestinal Alkaline Phosphatase (CIP) (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to dephosphorylation by alkaline calf intestinal phosphatase (New England Biolabs), followed by phenol-chloroform extraction of the dephosphorylated RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... calf intestinal alkaline phosphatase and assorted restriction enzymes were all purchased from NEB. Sequence optimized cDNA encoding cytokines and receptors were purchased from GenScript ...
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bioRxiv - Microbiology 2023Quote: ... RNAs were dephosphorylated using Calf Intestinal Alkaline Phosphatase (New England Biolabs, cat. M0525) then purified on denaturing 10% (w/v ...
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bioRxiv - Genomics 2023Quote: ... CROP-seq-opti was linearized via digestion with BsmBI and alkaline phosphatase (NEB) with PCR clean up in between both digestions ...
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bioRxiv - Microbiology 2023Quote: ... The transcripts were dephosphorylated with calf intestinal alkaline phosphatase (CIP, New England Biolabs) and then radioactively labeled at 5’ end with [γ-32P] ATP (Perkin Elmer ...
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bioRxiv - Microbiology 2023Quote: ... for dephosphorylation they were treated with Alkaline Phosphatase Calf Intestinal (New England Biolabs). Poly-I:C (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... and de-phosphorylated with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA). The ligation was performed using NEB T4 ligase (New England Biolabs ...