Labshake search
Citations for New England Biolabs :
151 - 200 of 9146 citations for Glutathione Colorimetric Detection Kit 200 Low Cuvettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: Thermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). Thermocycler was adjusted for heteroduplex formation as following ...
-
bioRxiv - Microbiology 2021Quote: ... mixtures for the detection of viral RNA were made by a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs, USA) in accordance with the manufacturer’s instructions and then placed in a thermocycler (BIO-RAD CFX96™ Real-Time System ...
-
bioRxiv - Neuroscience 2020Quote: ... as well as wildtype (WT) N2aC were assayed for mis-matched DNA pairs via T7 endonuclease assay (EnGen Mutation Detection Kit; New England Biolabs, Ipswich, MA) with PCR primers targeted towards our genomic region of interest (Integrated DNA technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... We designed spCas9 guide RNAs to target codon 12 of the human KRAS gene and selected the one with highest targeting efficiency determined by T7E1 mismatch assay (EnGen Mutation Detection Kit, New England Biolabs, Ipswich, MA). Truncated guide RNA was produced by PCR assembly of a guide RNA template (56 ...
-
bioRxiv - Microbiology 2021Quote: ... LAMP (Loop-Mediated Isothermal Amplification) reactions were performed with WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) (New England Biolabs) and an alternative set of primers (Supplementary Table S3) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 200 units RNaseH (NEB) and 20 μl of SUPERasIN ...
-
bioRxiv - Molecular Biology 2022Quote: ... 200 μM dNTPs (NEB), 1X Phusion® Buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... 200 μM dNTPs (NEB), 1X Phusion® Buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... 200 µM dNTPs (NEB), 0.5 µM forward primer ...
-
bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 viral RNA detection and quantification was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, Whitby, ON, Canada) on the Rotor-gene Q platform (Qiagen) ...
-
bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2024Quote: ... The soluble fraction of the lysate was used for affinity purification on HisPur Cobalt resin (Thermo, Cat#89964), Glutathione Agarose resin (Thermo, Cat#16100) or Amylose Resin (New England Biolabs, E8021), depending of the fusion tag ...
-
bioRxiv - Molecular Biology 2020Quote: ... Ribosomal RNA was depleted from 200 μg of the RNA using the NEBNext rRNA Depletion Kit (NEB E6310X) and then a cDNA library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530L) ...
-
bioRxiv - Genomics 2023Quote: ... 200 ng of sheared DNA was processed using the NEBNext Enzymatic Methyl-seq Kit (E7120; NEB, Ipswich, MA) following the manufacturer’s instructions for large insert libraries ...
-
bioRxiv - Biochemistry 2022Quote: ... Low-complexity ssDNA was synthesized in phi29 DNA polymerase reaction buffer (NEB M0269S), 500 μM dCTP and dTTP (NEB N0446S) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Quick-Load 1 kb Extend DNA ladder or Low Range PFG Marker (NEB).
-
bioRxiv - Biochemistry 2020Quote: ... Low-complexity ssDNA was synthesized in phi29 DNA polymerase reaction buffer (NEB M0269S), 500 μM dCTP and dTTP (NEB N0446S) ...
-
bioRxiv - Microbiology 2022Quote: ... and resolved by electrophoresis in TBE buffer with low range ssRNA ladder (NEB). Gels were post-stained with SYBR gold for 1 hr at room temperature and imaged using BioRad Gel Doc EZ Imager.
-
bioRxiv - Cell Biology 2022Quote: ... The amplicons (200 fmol) were first end-repaired with the NEBNext Ultra II End repair kit (New England Biolabs), then purified using AMPure XP beads (Beckman Coulter) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 200 units RNase H (NEB) and 20 μl of SUPERasIN (ThermoFisher) ...
-
bioRxiv - Biochemistry 2019Quote: ... 200 U/ml Nb.BssSI (NEB) and in-house produced thermus thermophilus RecJ exonuclease.43 1% exonuclease corresponds to a degradation rate of 22 nM/min for a 15-mer ssDNA at 44°C ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 200 U T4 ligase (NEB), 10 U BsaI-HFv2 (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... 200 μM each dNTP (NEB), 1 U Phusion DNA polymerase (NEB) ...
-
bioRxiv - Microbiology 2021Quote: ... The oligo was amplified in 25 individual low-cycle PCRs using Q5 polymerase (NEB).The amplicons were pooled and gel purified prior to cloning into a modified PASTN plasmid using 50 individual ligation reactions ...
-
bioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized using 200 ng total RNA with the ProtoScript® First Strand cDNA Synthesis kit (New England Biolabs) utilizing random hexamers ...
-
bioRxiv - Systems Biology 2020Quote: RNA-seq libraries were prepared using 200 ng of total RNA and the NEBNext rRNA Depletion Kit (NEB, Cat: E6310X), per manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... RNA-seq libraries were then prepared from 200 ng of RNA using the NEBNext Ultra II Directional RNA-seq kit (NEB). To quantitate the consistency of spike-in cell mixing for each individual sample ...
-
bioRxiv - Microbiology 2020Quote: ... electrophoresed and the 200-bp bands were excised from agarose gel slice using Monarch DNA Gel extraction Kit (NEB, #T1020S). Then ...
-
bioRxiv - Molecular Biology 2022Quote: The library for RNA-seq was prepared with 200 ng RNA input and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs (NEB), E7760L ...
-
bioRxiv - Genomics 2023Quote: ... 170-200 ng of library was indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The manufacturer’s protocol was followed with the following deviations ...
-
bioRxiv - Plant Biology 2021Quote: ... using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, MA, USA), Iso-Seq Express Oligo Kit ...
-
bioRxiv - Bioengineering 2023Quote: ... T4 Ligase and low molecular-weight DNA ladder were obtained from New England Biolabs (NEB). Methyltetrazine DBCO ...
-
bioRxiv - Developmental Biology 2023Quote: ... 7 μL of 2x NEBNext Library Quant Master Mix with 1:100 low ROX (NEB), and 1.5 μL ddH2O ...
-
bioRxiv - Systems Biology 2024Quote: ... Low complexity regions of JNK2α2 and ASK1 were deleted using Q5 Site-Directed Mutagenesis (NEB). Oligonucleotides used for cloning and site-directed mutagenesis are listed in the Table S2.
-
bioRxiv - Molecular Biology 2019Quote: ... 200 units λ protein phosphatase (NEB) was added to 10 μL of the suspension ...
-
bioRxiv - Genomics 2022Quote: ... 200 μ M GTP (NEB, N0450S)).The run-on reaction was stopped by adding Trizol LS (Life Technologies ...
-
bioRxiv - Genomics 2022Quote: ... and 200 U M.CviPI (NEB, #M0227L) were added and incubated at 37°C for 7.5 min ...
-
bioRxiv - Zoology 2021Quote: ... 200 μM of dNTPs (NE Biolabs), and 2 μl of DNA extract ...
-
bioRxiv - Genomics 2021Quote: ... and 200 U of M.CviPI (NEB) in 500 μl volume per 500,000 nuclei ...
-
bioRxiv - Microbiology 2020Quote: ... 200 units of PNGase F (NEB) was added into 50 μl of 50 mM NH4HCO3 in H2 18O (Aladdin ...
-
bioRxiv - Microbiology 2021Quote: ... 200 μM dNTP mix (NEB N0447L), 400 nM of 519F and 786R ...
-
bioRxiv - Neuroscience 2022Quote: ... Total RNA preparations were then used directly for qPCR (200 ng total RNA per reaction) using the Luna Universal One-Step RT-qPCR Kit (NEB, E3006) and target-specific primer/probe sets (ThermoFisher ...
-
bioRxiv - Synthetic Biology 2021Quote: Illumina sequencing libraries were generated from 100-200 ng BAC DNA using NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs E7805S) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2019Quote: ... 100-200 ng total RNA was used to prepare an RNAseq library using an mRNA isolation kit (New England BioLabs, E7490) and a NEBNext Ultra RNA library prep kit (New England BioLabs ...
-
bioRxiv - Genetics 2020Quote: ... The purified DNA (200 ng) was subject to library preparation using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB). Libraries with barcodes were pooled and sequenced on the Illumina HiSeq 1500 platform ...
-
bioRxiv - Molecular Biology 2022Quote: The library for EM-seq was prepared using 200 ng DNA input and the NEBNext Enzymatic Methyl-seq Kit (NEB, E7120S) following the manufacturer’s instructions for a standard insert (370-420 bp) ...
-
bioRxiv - Molecular Biology 2022Quote: The library for RNA-seq was prepared with 200 ng RNA input and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs (NEB), E7760L ...
-
bioRxiv - Genomics 2023Quote: ... Sequencing libraries for genome wide DNA methylation profiles were generated from 200 ng of genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (New England Biolabs). These libraries were sequenced on an Illumina NextSeq 2000 to generate 100 bp paired end reads.
-
bioRxiv - Developmental Biology 2021Quote: cDNA was synthesized using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (E6421, NEB, USA) following PacBio Iso-Seq template preparation guidelines ...
-
bioRxiv - Molecular Biology 2020Quote: ... and electrophoresed alongside a low molecular weight ladder (range 25 to 766 base pairs, N3233, NEB) or HyperLadder 1 kb (range 200 bp to 10 kb ...