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Citations for New England Biolabs :
51 - 100 of 264 citations for Galectin 9 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The glutathione S-transferase (GST) tag was cleaved using Factor Xa Protease (New England Biolabs, Ipswich, MA, USA) in accordance with the manufacturer’s instruction.
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bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... AtPAXXL228E fused to hexa-histidine followed by a GST tag (pGAT3-atpaxxL228E) was expressed in BL21(DE3) (NEB) and purified using a 5 ml GSTrap HP column (Cytiva) ...
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bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs). Regularly ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... HEK293 libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat. #E7770) or NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760) ...
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bioRxiv - Genetics 2019Quote: ... for 9 h and treated with 2 μl of calf intestinal alkaline phosphatase (NEB) at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Biophysics 2021Quote: ... Amplified fragments were cloned into pET (His6) and pGEX (GST) based vectors using Gibson cloning method (NEB builder, E5520S). Plasmids used in this study is listed in Table S1.
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bioRxiv - Biochemistry 2020Quote: pGEX6p2-GST-GβL was cloned from pRK5-HA-GβL into pGEX6p2-GST and was transformed into BL21 (DE3) cells (New England Biolabs) and purified using Glutathione Sepharose beads (45000139 ...
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bioRxiv - Neuroscience 2020Quote: ... To perform in vitro phosphorylation 5 μg of purified GST-NL2CT (WT or S714D) fusion protein was incubated with 2.5kUnits purified catalytic subunit of PKA (NEB) supplemented with 200 μM ATP at 30°C for the indicated time points.
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of immunopurified GST-tagged WRN fragment was phosphorylated in vitro by Casein Kinase II (New England Biolabs) in the presence of 1X NEBuffer (50mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by 9 cycles of PCR enrichment using NEB High fidelity PCR kit (NEB, M0541S). The quality of ATAC libraries was assessed with Agilent Bioanalyzer with DNA High Sensitivity kit (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... 29 μl of purified modified RNA was added to 100 ng Random Primer 9 (NEB) and 0.2 mM of each dNTP ...
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bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... the protein mix was incubated for 1 h at 4° C in 150 µL pull-down buffer supplemented with 15 µL glutathione agarose resin for GST pull-downs or amylose resin (New England Biolabs) for MBP pull-downs ...
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bioRxiv - Cell Biology 2021Quote: ... and pGEX-GST-p130593-790 R680A L682A were generated by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... GST fusion proteins of the CTD and its mutants CTD-AA and CTD-YA were phosphorylated by Cdc2 kinase (NEB) using 32P-γ-ATP for 30 minutes at 30°C before incubated with immunoprecipitated CoAA or hnRNP M ...
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bioRxiv - Cell Biology 2021Quote: ... and ligated to linearized vector pGex-6p-1 (GST) or pET28-SUMO (His) also cut with the same restriction enzymes using T4 DNA ligase (NEB).
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bioRxiv - Biophysics 2023Quote: ... and cloned into Sal I-digested GST vector pGEX-6P-1 (Cytiva) by NEBuilder HiFi DNA Assembly cloning (New England BioLabs). To express the GST fusion protein ...
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bioRxiv - Microbiology 2023Quote: Epitope-tagged constructs using the GST tag or the 3X FLAG tag were generated using the HiFi DNA Assembly cloning kit (NEB). MntS was cloned into pGEX-6-1 and the GST-MntS fusion was subcloned into pKT25c such that the resulting plasmid lacked the T25 region ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were separated from lysates and incubated with affinity beads at 4°C overnight (His beads: Ni-NTA from QIAGEN; GST beads: glutathione-agarose beads from ROTH; MBP beads: amylose beads from NEB). Protein-binding beads were collected by centrifugation and washed several times with washing buffer (His washing buffer ...
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bioRxiv - Plant Biology 2023Quote: AtXRCC4 fused to a hexa-histidine followed by a GST tag (pGAT3-atxrcc4) was expressed in BL21(DE3) cells (NEB), The proteins were purified using GST sepharose (Cytiva) ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cell suspensions (1×106/100 μL) were treated with DNAse I (0.2 U/μL, NEB M0303S; NEB DNaseI buffer) or Benzonase (25 U/μL ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cell suspensions (1×106/100 μL) were treated with DNAse I (0.2 U/μL, NEB M0303S; NEB DNaseI buffer) or Benzonase (25 U/μL ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification (8-9 cycles) was performed with 10 μL Fusion HF Buffer (New England Biolabs), 3.125 μL 10uM TruSeq Primer 1 ...
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bioRxiv - Genomics 2019Quote: ... dried and re-dissolved in 9 µl of 1x ligation buffer (New England Biolabs, Ipswich, MA). To this was added ...
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bioRxiv - Genomics 2021Quote: ... 500 ng of DNA was dissolved in 9 μL of 1 × CutSmart buffer (New England Biolabs) and sheared by pipetting 30 times with a pipette set at 8 μL with a P2/P10 tip ...
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bioRxiv - Microbiology 2020Quote: ... and performed 6–9 cycles of PCR with the NEBNext Ultra II Q5 Master Mix (NEB) using Illumina P7 and the Seq-Well P5-TSO hybrid primer (Gierahn et al. ...
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bioRxiv - Genomics 2023Quote: ... Libraries were amplified for 9-12 cycles using Q5 Hot-Start Mastermix (NEB Cat No M0294L) and primers that added the full Illumina adaptor sequences ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into pGex6P-1 in-frame with an N-terminal 3C-cleavable GST tag using HiFi assembly (New England Biolabs, USA).
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bioRxiv - Microbiology 2020Quote: ... Total RNA was collected at 9 h post-IFN treatment using Monarch Total RNA Miniprep Kits (NEB). RNA was prepped for RNA sequencing submission using the NEBNext Poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... (9)C=cell barcode) was used for single-stranded synthesis and Second Strand Synthesis Module (NEB, #E6111) was used for double-stranded cDNA synthesis ...
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bioRxiv - Genomics 2023Quote: ... at 10 pmol each and then amplified in 9 cycles using Phusion DNA polymerase (New England Biolabs) from primers that added flanking overlaps to tiles allowing Infusion cloning in a screening vector ...
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bioRxiv - Physiology 2022Quote: ... Diluted lysate was then incubated with 9 ml of packed amylose resin (catalog no. E8021S, New England Biolabs) and incubated at 4°C for 2h with gentle rotation ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 µg of total protein (9 µl of lysate) were treated with Endo Hf (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Pre-crRNA arrays (Supplementary Table 9) were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB). T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB) ...
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bioRxiv - Systems Biology 2024Quote: ... human (E6320, New England Biolabs, USA) and sequenced on a MiSeq (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Genetics 2019Quote: ... The 3′-UTR sequences of both genes were then amplified from genomic DNA obtained from HEK293 cells with Phusion® High-Fidelity DNA Polymerases (NEB, Ipswich, MA) and cloned into the multiple cloning site of the pMIR-REPORT luciferase miRNA expression vector (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... were added to each sample in a 9-cycle PCR reaction prepared by adding 12.5 μl Phusion 2X Master Mix (NEB) and 2 μl each of Nextera XT S5 and N7 adapters ...
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bioRxiv - Microbiology 2019Quote: ... 1 pmol of DNA template was mixed with 10 pmol of oligonucleotide 9 labeled with Yakima Yellow and 1µL of HemoKlen Taq DNA Polymerase (New England Biolabs) in a 6µL reaction mixture containing 50 mM Tris-HCl pH 9.0 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µl was added to a 9 µl Luna® Universal One-Step RT-qPCR reaction (NEB, Cat# E3005L). RT-qPCR was carried out to manufacturer specifications in a CFX96 Touch Real Time PCR Machine (Bio-Rad) ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 9 cDNA libraries were prepared with an NEBNext Ultra II RNA Library Prep kit (NEB, cat:E7770S) and sequenced with an Illumina HiSeq4000 ...
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bioRxiv - Bioengineering 2020Quote: ... Then 5 μl extracted RNA was fragmented at 94°C for 9 minutes by using 0.2× fragmentation buffer (NEB) before conducting the nanoswitch detection ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Microbiology 2019Quote: ... falciparum and human histones (New England Biolabs) (6µg) ...
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bioRxiv - Microbiology 2021Quote: ... 40 U RNase Inhibitor Human Placenta (NEB), 50 pmol Oligo d(T)23 (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... Human CK2 was obtained from NEB (P6010S). IE2-NTD was phosphorylated in buffer provided by the manufacturer which contains 50mM Tris pH 7.5 ...