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Citations for New England Biolabs :
1 - 50 of 4962 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: The FH1-tUTG inducible knockdown vector was digested using BsmBII (NEW ENGLAND BioLabs, #R0580S) and XhoI (NEW ENGLAND BioLabs ...
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant protein of AP2 domains fused with MBP (MBP::PbSIP2) was purified using amylose resin (NEB) and recovered in 10 mM maltose solution.
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bioRxiv - Microbiology 2024Quote: ... or Inc proteins lacking the transmembrane domain were cloned into the pMALc-5VT vector (Protein and Crystallography Facility, UIOWA) using NotI (NEB Cat#R3189L) and SalI-HF ...
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bioRxiv - Cell Biology 2021Quote: ... The digested plasmid was mixed with the different PCR-amplified sensory domains-ORFs containing overlapping ends using the Gibson Assembly method (NEB). AtLEA4-2 ...
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bioRxiv - Cell Biology 2023Quote: MBP-tagged RH domain was isolated via passing cleared lysate over a gravity amylose resin column containing 5 mL of settled resin (NEB). Column was washed with 150 mL of buffer before eluting protein with wash buffer supplemented with 20 mM maltose ...
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bioRxiv - Genetics 2024Quote: ... 1 ug of plasmid pools containing TF domains were digested in two steps: first with 2.5 uL of BsaI restriction enzyme (New England Biolabs, NEB) in 50 uL total volume (with CutSmart Buffer ...
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bioRxiv - Neuroscience 2022Quote: ... so the bead-bound proteins were dephosphorylated in wash buffer containing 1:50 Lambda Protein Phosphatase (New England Biolabs) and 1 mM MnCl2 to make the phosphosites more accessible for a kinase assay ...
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bioRxiv - Immunology 2024Quote: ... containing protein inhibitor cocktail (New England Biolabs). Cell lysates were incubated on ice for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: pMAL-c4E vectors carrying in-frame fusions of the EFR cytoplasmic domain with the N-terminal maltose-binding protein (MBP) tag were transformed into Rosetta 2 cells (NEB) for recombinant protein expression ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Physiology 2020Quote: ... 1 nl of CRISPR mixture containing 2,4 μg/μl of gRNA and 0.5 μl Cas9 protein (NEB) was injected in one-cell stage embryos and raised for 24 hours ...
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bioRxiv - Biophysics 2020Quote: ... for the substrate-binding domain mutants and βamHI-AlwnI (NEB) for the transmembrane domain mutants (Appendix) ...
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bioRxiv - Plant Biology 2023Quote: Mutants in the CG-1 domain were created using the Q5 site-directed mutagenesis kit (New England Biolabs, USA) as per manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... A mixture containing 600 ng/uL of Cas9 protein (NEB: M0386) and sgRNA complex at a 1:1 ratio was generated with a total volume of 3 uL ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein-containing fractions were pooled and incubated with Chitin Resin (NEB) for 30 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The KHNYN domain swap constructs were generated by HiFi assembly (NEB) using amplicons obtained using Q5 DNA polymerase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: The kinase domain variant library was digested with PstI-HF (NEB) and NdeI-HF (NEB ...
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bioRxiv - Microbiology 2020Quote: ... the C-terminal domain was removed by digestion with Endoproteinase LysC (NEB) at a 1:800 LysC:substrate ratio by mass for 1-2h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: DNMT3A PWWP domain was expressed in BL21(DE3) cells (New England Biolabs) using the same protocol as above ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: Purified DNA was amplified with human-or mouse-specific primers covering the RBM20 RS-domain mutation hotspot with Nextera-compatible adapters (Sup. Table 1) using Q5®Hot Start High-Fidelity 2X Master Mix (NEB). One microliter of a 1:100 dilution was used for a second PCR attaching sample-specific index barcodes (Nextera XT Index Kit v2 Set A ...
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bioRxiv - Biochemistry 2023Quote: ... the REC114-MEI4 protein-containing supernatants were loaded onto an Amylose resin (NEB). After extensive washing with a buffer containing 20mM Tris pH 8 ...
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bioRxiv - Immunology 2023Quote: ... containing 1 mM sodium orthovanadate (NEB, # P0758S). The membrane from the transwell inserts was cut using forceps and placed in 300 µL of 1X cell lysis buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 1 volume of λ-Phosphatase (NEB) with 1 volume of water ...
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bioRxiv - Neuroscience 2021Quote: ... C4 domain sequence was removed with the Q5 site-directed mutagenesis kit (NEB). Mouse prolyl 4-hydroxylase alpha- and beta-chain (P4HA1 and P4HB ...
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: Domain swap constructs were generated using Gibson assembly Master Mix (New England BioLabs). The coding sequences of RLP42 and RLP40 were cloned into pDONR207 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... CD45 catalytic domain DNA constructs were transformed into the BL21 (DE3) strain (NEB) Escherichia coli and plated on LB agar with ampicillin (100 mg/ml) ...
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Biochemistry 2021Quote: ... The MCS-intein-chitin binding domain of vector pTXB1 (New England Biolabs, Ipswitch, MA) was then inserted between sites NheI and Kpn2I ...
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bioRxiv - Immunology 2021Quote: ... CD4 domain deletion mutants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufactureŕs protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The domain swap constructs were assembled using the NEBuilder HiFi DNA assembly kit (NEB). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... 28 novel RNF43 RING domain variants were generated using Q5 mutagenesis (New England Biolabs). All ZNRF3 and RNF43 expression plasmids were full-length sequence verified ...
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bioRxiv - Biophysics 2022Quote: ... Deletion of N2B-us folding domain was accomplished by PCR and DPN1 (NEB, R0176S) reaction with subsequent KLD (NEB KLD Enzyme Mix ...
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bioRxiv - Synthetic Biology 2024Quote: ... Mutation of ORF2 RT domain was introduced using Q5 site-directed mutagenesis kit (NEB). Deletion of ORF2 EN domain was performed by PCR and ligation cloning.
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bioRxiv - Genomics 2024Quote: ... a ligation mix containing 1× T4 buffer (NEB) and 20 U/μl T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2022Quote: For crystallization the Ioc4 PWWP domain (aa 1-178; Ioc4PWWP) was cloned into a modified pMAL-c2X vector (New England Biolabs, Liu et al., 2001) in order to produce a fusion protein with an N-terminal maltose-binding protein (MBP) ...
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bioRxiv - Biochemistry 2022Quote: ... Association occurred in samples containing ACE2- fusion proteins either without or with TEV protease (NEB) treatment ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
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bioRxiv - Cell Biology 2020Quote: GST or GST-tagged SH3 domains were expressed in BL21 (DE3) E.coli (New England Biolabs). Cells were lysed by sonication in presence of lysozyme (Affymetrix) ...
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bioRxiv - Cell Biology 2023Quote: ... and mutant Pacer RH domain PCR product were mixed with Gibson Assembly master mix (NEB) in a 1:3:3 molar ratio prior to incubation at 50 °C for 15 minutes and transformation into XL10 E ...
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bioRxiv - Biophysics 2023Quote: ... The DNA of the SL5 domain was then digested using MlyI (New England Biolabs #R0610) and the DNA of the SL5-6 domains was then digested using BsaI (New England Biolabs #R0535 ...
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bioRxiv - Cell Biology 2024Quote: ... and the EndoA2 SH3 domain was cloned into pMAL-c2g (New England Biolabs, MBP-tagging) (11) ...
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bioRxiv - Cell Biology 2024Quote: ... into vectors containing a fluorescent protein and a selection cassette using Gibson Assembly (New England Biolabs), as thoroughly detailed by Dickinson et al ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were then coupled to protein G beads (New England Biolabs). After beads were washed ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were detected using anti-MBP antibody (anti-MBP NEB e8032s) and HRP conjugated secondary antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... The R462M point mutation in the PTP domain was introduced by Q5 site-directed mutagenesis (NEB). pCS2+ constructs were linearized using NotI and transcribed in vitro (mMessage machine SP6 ...
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bioRxiv - Biochemistry 2020Quote: ... Point mutations and domain deletions were generated using QuickChange site-directed mutagenesis with Q5 Polymerase (NEB). All plasmids were verified by DNA sequencing.
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bioRxiv - Immunology 2021Quote: ... Ig kappa or Ig lambda constant domains using the Quick Ligase cloning system (New England BioLabs) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... hnRNPA1 effector domain was amplified from HEK293T cDNA using the Q5 High fidelity polymerase (#M0491S, NEB). Overhanging BsWI sites were added to the oligonucleotides for cloning into the dCasRx plasmid with Quick Ligation kit (M2200S ...